Product: RAB33B Antibody
Catalog: DF9826
Description: Rabbit polyclonal antibody to RAB33B
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.: 26 kDa; 26kD(Calculated).
Uniprot: Q9H082
RRID: AB_2843020

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(89%)
Clonality:
Polyclonal
Specificity:
RAB33B Antibody detects endogenous levels of total RAB33B.
RRID:
AB_2843020
Cite Format: Affinity Biosciences Cat# DF9826, RRID:AB_2843020.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZP434G099; MGC138182; RAB 33B; RAB33B; RAB33B member RAS oncogene family; Ras related protein Rab 33B; Ras related protein Rab33B; Ras-associated protein RAB33B; Ras-related protein Rab-33B; RB33B_HUMAN; SMC2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MAEEMESSLEASFSSSGAVSGASGFLPPARSRIFKIIVIGDSNVGKTCLTYRFCAGRFPDRTEATIGVDFRERAVEIDGERIKIQLWDTAGQERFRKSMVQHYYRNVHAVVFVYDMTNMASFHSLPSWIEECKQHLLANDIPRILVGNKCDLRSAIQVPTDLAQKFADTHSMPLFETSAKNPNDNDHVEAIFMTLAHKLKSHKPLMLSQPPDNGIILKPEPKPAMTCWC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
89
Xenopus
67
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H082 As Substrate

Site PTM Type Enzyme
T89 Phosphorylation
S98 Phosphorylation
K149 Ubiquitination
K165 Ubiquitination

Research Backgrounds

Function:

Protein transport. Acts, in coordination with RAB6A, to regulate intra-Golgi retrograde trafficking. It is involved in autophagy, acting as a modulator of autophagosome formation.

Subcellular Location:

Golgi apparatus membrane>Lipid-anchor. Golgi apparatus>cis-Golgi network.
Note: Under starvation conditions punctate RAB33B-positive structures are often observed in the cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with ATG16L. Interaction with ATG16L is important for autophagosome formation (By similarity). Interacts with RIC1 (via C-terminus domain); the interaction is direct with a preference for RAB33B-GTP. Interacts with RGP1.

Family&Domains:

Belongs to the small GTPase superfamily. Rab family.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

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For Research Use Only.
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