RAB22A Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using RAB22A Antibody.

Product:	RAB22A Antibody
Catalog:	DF9820
Description:	Rabbit polyclonal antibody to RAB22A
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	22 kDa; 22kD(Calculated).
Uniprot:	Q9UL26
RRID:	AB_2843014

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(92%), Dog(92%), Chicken(83%)

Clonality:

Polyclonal

Specificity:

RAB22A Antibody detects endogenous levels of total RAB22A.

RRID:

AB_2843014
Cite Format: Affinity Biosciences Cat# DF9820, RRID:AB_2843014.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

3732413A17Rik; AI662177; AW319644; AW550514; E130120E14Rik; GTP binding protein RAB22A; MGC16770; OTTMUSP00000017605; Rab 22; RAB 22A; Rab-22; Rab22; RAB22A; RAB22A member RAS oncogene family; Ras related protein Rab 22A; Ras related protein Rab22A; Ras-related protein Rab-22A; RB22A_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q9UL26(RB22A_HUMAN) >>Visit HPA database.

Gene(ID):

RAB22A(57403)

Sequence:

Q9UL26 RB22A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MALRELKVCLLGDTGVGKSSIVWRFVEDSFDPNINPTIGASFMTKTVQYQNELHKFLIWDTAGQERFRALAPMYYRGSAAAIIVYDITKEETFSTLKNWVKELRQHGPPNIVVAIAGNKCDLIDVREVMERDAKDYADSIHAIFVETSAKNAININELFIEISRRIPSTDANLPSGGKGFKLRRQPSEPKRSCC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Horse

Dog

Rabbit

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UL26 As Substrate

Q9UL26

Site	PTM Type	Source
K7	Ubiquitination	Uniprot
T14	Phosphorylation	Uniprot
S19	Phosphorylation	Uniprot
S20	Phosphorylation	Uniprot
K101	Acetylation	Uniprot
K101	Ubiquitination	Uniprot
S175	Phosphorylation	Uniprot

Research Backgrounds

Q9UL26_RB22A_HUMAN

Function:

Plays a role in endocytosis and intracellular protein transport. Mediates trafficking of TF from early endosomes to recycling endosomes. Required for NGF-mediated endocytosis of NTRK1, and subsequent neurite outgrowth. Binds GTP and GDP and has low GTPase activity. Alternates between a GTP-bound active form and a GDP-bound inactive form.

Subcellular Location:

Endosome membrane>Lipid-anchor. Cell membrane>Lipid-anchor. Early endosome. Late endosome. Cell projection>Ruffle. Cytoplasmic vesicle. Cytoplasmic vesicle>Phagosome. Cytoplasmic vesicle>Phagosome membrane>Lipid-anchor>Cytoplasmic side.
Note: Recruited to phagosomes containing S.aureus or M.tuberculosis.

Subunit Structure:

Interacts directly with ZFYVE20 (By similarity). Binds EEA1 (By similarity). Interacts (in its GTP-bound form) with RABGEF1. Interacts (in its GTP-bound form) with RINL.

Family&Domains:

Belongs to the small GTPase superfamily. Rab family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)

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RAB22A Antibody - #DF9820