REPS2 Antibody - #DF9801

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Product: REPS2 Antibody
Catalog: DF9801
Description: Rabbit polyclonal antibody to REPS2
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 72 kDa; 72kD(Calculated).
Uniprot: Q8NFH8
RRID: AB_2842995

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(88%), Horse(89%), Sheep(88%), Rabbit(100%), Dog(89%)
Clonality:
Polyclonal
Specificity:
REPS2 Antibody detects endogenous levels of total REPS2.
RRID:
AB_2842995
Cite Format: Affinity Biosciences Cat# DF9801, RRID:AB_2842995.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

OTTHUMP00000022995; Partner of Ral binding protein 1; Partner of RalBP1; POB1; RALBP1 associated Eps domain containing 2; RALBP1 interacting protein 2; RALBP1-associated Eps domain-containing protein 2; REPS 2;

Immunogens

Immunogen:
Uniprot:

Q8NFH8(REPS2_HUMAN) >>Visit HPA database.

Gene(ID):
REPS2(9185)
Expression:
Q8NFH8 REPS2_HUMAN:

Expressed at high levels in the cerebrum, cerebellum, lung, kidney, and testis. Weakly expressed in the kidney. Relatively highly expressed in androgen-dependent as compared to androgen-independent prostate cancer cell lines and xenografts. Isoform 2 is down-regulated during progression of prostate cancer.

Sequence:
MEAAAAAAAAAAAAAAAGGGCGSGPPPLLLSEGEQQCYSELFARCAGAAGGGPGSGPPEAARVAPGTATAAAGPVADLFRASQLPAETLHQITELCGAKRVGYFGPTQFYIALKLIAAAQSGLPVRIESIKCELPLPRFMMSKNDGEIRFGNPAELHGTKVQIPYLTTEKNSFKRMDDEDKQQETQSPTMSPLASPPSSPPHYQRVPLSHGYSKLRSSAEQMHPAPYEARQPLVQPEGSSSGGPGTKPLRHQASLIRSFSVERELQDNSSYPDEPWRITEEQREYYVNQFRSLQPDPSSFISGSVAKNFFTKSKLSIPELSYIWELSDADCDGALTLPEFCAAFHLIVARKNGYPLPEGLPPTLQPEYLQAAFPKPKWDCQLFDSYSESLPANQQPRDLNRMEKTSVKDMADLPVPNQDVTSDDKQALKSTINEALPKDVSEDPATPKDSNSLKARPRSRSYSSTSIEEAMKRGEDPPTPPPRPQKTHSRASSLDLNKVFQPSVPATKSGLLPPPPALPPRPCPSQSEQVSEAELLPQLSRAPSQAAESSPAKKDVLYSQPPSKPIRRKFRPENQATENQEPSTAASGPASAATMKPHPTVQKQSSKQKKAIQTAIRKNKEANAVLARLNSELQQQLKEVHQERIALENQLEQLRPVTVL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
100
Horse
89
Dog
89
Bovine
88
Sheep
88
Chicken
63
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8NFH8 As Substrate

Site PTM Type Enzyme
Y103 Phosphorylation
T107 Phosphorylation
Y110 Phosphorylation
S121 Phosphorylation
S187 Phosphorylation
S191 Phosphorylation
S195 Phosphorylation
S198 Phosphorylation
S199 Phosphorylation
Y212 Phosphorylation
S213 Phosphorylation
S217 Phosphorylation
S218 Phosphorylation
S254 Phosphorylation
S260 Phosphorylation
S304 Phosphorylation
T446 Phosphorylation
S459 Phosphorylation
S461 Phosphorylation
Y462 Phosphorylation
S463 Phosphorylation P06493 (CDK1)
S464 Phosphorylation
S466 Phosphorylation
T479 Phosphorylation
S489 Phosphorylation
S492 Phosphorylation
S493 Phosphorylation
S503 Phosphorylation
S509 Phosphorylation
S544 Phosphorylation
Y558 Phosphorylation
S563 Phosphorylation
K610 Acetylation
S631 Phosphorylation

Research Backgrounds

Function:

Involved in growth factor signaling through its influence on the Ral signaling pathway.

PTMs:

EGF stimulates phosphorylation on Tyr-residues and induces complex formation with EGF receptor through an adapter protein such as GRB2.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed at high levels in the cerebrum, cerebellum, lung, kidney, and testis. Weakly expressed in the kidney. Relatively highly expressed in androgen-dependent as compared to androgen-independent prostate cancer cell lines and xenografts. Isoform 2 is down-regulated during progression of prostate cancer.

Subunit Structure:

Interacts with ASAP1 and this complex can bind paxillin. May form a ternary complex with RALBP1 and ASAP1 (By similarity). Interacts with RALBP1 and GRB2. Binding to RALBP1 does not affect the Ral-binding activity of the latter. It can form a ternary complex with activated Ral and RALBP1. Binds EPN1.

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