RAB11FIP5 Antibody - #DF9796

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Product: RAB11FIP5 Antibody
Catalog: DF9796
Description: Rabbit polyclonal antibody to RAB11FIP5
Application: WB IHC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Dog
Mol.Wt.: 70 kDa; 70kD(Calculated).
Uniprot: Q9BXF6
RRID: AB_2842990

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
RAB11FIP5 Antibody detects endogenous levels of total RAB11FIP5.
RRID:
AB_2842990
Cite Format: Affinity Biosciences Cat# DF9796, RRID:AB_2842990.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

GAF 1; Gaf-1; GAF1; gamma SNAP associated factor 1; Gamma-SNAP-associated factor 1; KIAA0857; Phosphoprotein pp75; pp75; RAB11 family interacting protein 5 (class I); Rab11 family-interacting protein 5; Rab11 FIP5; Rab11 interacting protein; Rab11 interacting protein Rip11; Rab11-FIP5; Rab11-interacting protein Rip11; Rab11fip5; RFIP5_HUMAN; RIP11;

Immunogens

Immunogen:
Uniprot:

Q9BXF6(RFIP5_HUMAN) >>Visit HPA database.

Gene(ID):
RAB11FIP5(26056)
Expression:
Q9BXF6 RFIP5_HUMAN:

Detected at low levels in heart, brain, placenta, lung, liver, adipocytes, kidney, spleen, skeletal muscle and pancreas.

Sequence:
MALVRGAEPAAGPSRWLPTHVQVTVLRARGLRGKSSGAGSTSDAYTVIQVGREKYSTSVVEKTHGCPEWREECSFELPPGALDGLLRAQEADAGPAPWAASSAAACELVLTTMHRSLIGVDKFLGQATVALDEVFGAGRAQHTQWYKLHSKPGKKEKERGEIEVTIQFTRNNLSASMFDLSMKDKPRSPFSKIRDKMKGKKKYDLESASAILPSSAIEDPDLGSLGKMGKAKGFFLRNKLRKSSLTQSNTSLGSDSTLSSASGSLAYQGPGAELLTRSPSRSSWLSTEGGRDSAQSPKLFTHKRTYSDEANQMRVAPPRALLDLQGHLDAASRSSLCVNGSHIYNEEPQGPVRHRSSISGSLPSSGSLQAVSSRFSEEGPRSTDDTWPRGSRSNSSSEAVLGQEELSAQAKVLAPGASHPGEEEGARLPEGKPVQVATPIVASSEAVAEKEGARKEERKPRMGLFHHHHQGLSRSELGRRSSLGEKGGPILGASPHHSSSGEEKAKSSWFGLREAKDPTQKPSPHPVKPLSAAPVEGSPDRKQSRSSLSIALSSGLEKLKTVTSGSIQPVTQAPQAGQMVDTKRLKDSAVLDQSAKYYHLTHDELISLLLQRERELSQRDEHVQELESYIDRLLVRIMETSPTLLQIPPGPPK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BXF6 As Substrate

Site PTM Type Enzyme
R5 Methylation
S116 Phosphorylation
T165 Phosphorylation
S174 Phosphorylation
S176 Phosphorylation
S181 Phosphorylation
S188 Phosphorylation
S191 Phosphorylation
S207 Phosphorylation
S209 Phosphorylation
S224 Phosphorylation
K232 Methylation
S254 Phosphorylation
T276 Phosphorylation
S278 Phosphorylation
S280 Phosphorylation
S282 Phosphorylation
S283 Phosphorylation
S286 Phosphorylation
T287 Phosphorylation
S293 Phosphorylation
S296 Phosphorylation
T305 Phosphorylation
Y306 Phosphorylation
S307 Phosphorylation Q15208 (STK38)
S332 Phosphorylation
S341 Phosphorylation
Y344 Phosphorylation
S356 Phosphorylation
S357 Phosphorylation
S359 Phosphorylation
S361 Phosphorylation
S364 Phosphorylation
S365 Phosphorylation
S367 Phosphorylation
S372 Phosphorylation
S373 Phosphorylation
S376 Phosphorylation
S391 Phosphorylation
S393 Phosphorylation
S395 Phosphorylation
S396 Phosphorylation
S397 Phosphorylation
S418 Phosphorylation
T438 Phosphorylation
S473 Phosphorylation
S481 Phosphorylation
S482 Phosphorylation
S494 Phosphorylation
S498 Phosphorylation
S499 Phosphorylation
S500 Phosphorylation
T519 Phosphorylation
S523 Phosphorylation
S538 Phosphorylation
S544 Phosphorylation
S547 Phosphorylation
S549 Phosphorylation
S553 Phosphorylation
K558 Ubiquitination
K560 Ubiquitination
S564 Phosphorylation
K583 Ubiquitination
K586 Ubiquitination
S588 Phosphorylation
S628 Phosphorylation
Y629 Phosphorylation

Research Backgrounds

Function:

Rab effector involved in protein trafficking from apical recycling endosomes to the apical plasma membrane. Involved in insulin granule exocytosis. May regulate V-ATPase intracellular transport in response to extracellular acidosis.

PTMs:

Phosphorylated on serine and threonine residues. Phosphorylation at Ser-357 is PKA-dependent.

Subcellular Location:

Cytoplasm. Recycling endosome membrane>Peripheral membrane protein. Early endosome membrane>Peripheral membrane protein. Golgi apparatus membrane>Peripheral membrane protein. Cytoplasmic vesicle>Secretory vesicle membrane>Peripheral membrane protein. Mitochondrion membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected at low levels in heart, brain, placenta, lung, liver, adipocytes, kidney, spleen, skeletal muscle and pancreas.

Subunit Structure:

Forms a heterooligomeric complex with RAB11FIP4. Binds NAPG and TROVE2. Binds RAB11A that has been activated by GTP binding.

Family&Domains:

Binds to vesicles enriched in neutral phospholipids via its C2 domain. The interaction is favored by Mg(2+) rather than Ca(2+).

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

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