RAB11FIP1 Antibody - #DF9795

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Product: RAB11FIP1 Antibody
Catalog: DF9795
Description: Rabbit polyclonal antibody to RAB11FIP1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Xenopus
Mol.Wt.: 137 kDa; 137kD(Calculated).
Uniprot: Q6WKZ4
RRID: AB_2842989

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
RAB11FIP1 Antibody detects endogenous levels of total RAB11FIP1.
RRID:
AB_2842989
Cite Format: Affinity Biosciences Cat# DF9795, RRID:AB_2842989.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ22524; FLJ22622; HGNC:30265; MGC78448; No Rab11 binding protein 1A; NOEL1A; Rab coupling protein; Rab effector protein; Rab interacting recycling protein; Rab-coupling protein; RAB11 family interacting protein 1 (class I); Rab11 family interacting protein 1D; Rab11 family-interacting protein 1; Rab11 FIP1; Rab11-FIP1; RAB11FIP1; RCP; RFIP1_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q6WKZ4(RFIP1_HUMAN) >>Visit HPA database.

Gene(ID):
RAB11FIP1(80223)
Expression:
Q6WKZ4 RFIP1_HUMAN:

Isoform 2 is expressed in brain, heart, testis, lung, spleen, ovary and small intestine.

Sequence:
MSLMVSAGRGLGAVWSPTHVQVTVLQARGLRAKGPGGTSDAYAVIQVGKEKYATSVSERSLGAPVWREEATFELPSLLSSGPAAAATLQLTVLHRALLGLDKFLGRAEVDLRDLHRDQGRRKTQWYKLKSKPGKKDKERGEIEVDIQFMRNNMTASMFDLSMKDKSRNPFGKLKDKIKGKNKDSGSDTASAIIPSTTPSVDSDDESVVKDKKKKSKIKTLLSKSNLQKTPLSQSMSVLPTSKPEKVLLRPGDFQSQWDEDDNEDESSSASDVMSHKRTASTDLKQLNQVNFTLPKKEGLSFLGGLRSKNDVLSRSNVCINGNHVYLEQPEAKGEIKDSSPSSSPSPKGFRKKHLFSSTENLAAGSWKEPAEGGGLSSDRQLSESSTKDSLKSMTLPSYRPAPLVSGDLRENMAPANSEATKEAKESKKPESRRSSLLSLMTGKKDVAKGSEGENPLTVPGREKEGMLMGVKPGEDASGPAEDLVRRSEKDTAAVVSRQGSSLNLFEDVQITEPEAEPESKSEPRPPISSPRAPQTRAVKPRLEVSPEAQPTARLPSPTDSPSSLPPLPSSSGQASVPSELGHGADTQSSESPSVFSSLSSPIAAPISTSTPIESWPLVDRGQAKSEGPPLLPKAELQTESLTPVPNSGSSALGSLFKQPSFPANKGTEDSLMGRTRETGTEKNTSSLELEESLPEQPETGRQEEELPRFPCKKQDYSPSSGEAQEVPFALSLSSDGAVSPVGELAAGGDRDLESQAGSLVESKARDAAEEVAPPLPMGASVPSIDSMMRKLEEMGLNLRKDQKKTKKRVSFSEQLFTEEAVAGAALLVEGHSSCPQELNPAWSVAGNASDGEPPESPHAEDSERESVTTPGPATCGAPASPADHLLLPSQEESFSEVPMSEASSAKDTPLFRMEGEDALVTQYQSKASDHEGLLSDPLSDLQLVSDFKSPIMADLNLSLPSIPEVASDDERIDQVEDDGDQVEDDGETAKSSTLDIGALSLGLVVPCPERGKGPSGEADRLVLGEGLCDFRLQAPQASVTAPSEQTTEFGIHKPHLGKSSSLDKQLPGPSGGEEEKPMGNGSPSPPPGTSLDNPVPSPSPSEIFPVTHSFPSSAHSDTHHTSTAESQKKATAEGSAGRVENFGKRKPLLQAWVSPSETHPVSAQPGAGTGSAKHRLHPVKPMNAMATKVANCSLGTATIISENLNNEVMMKKYSPSDPAFAYAQLTHDELIQLVLKQKETISKKEFQVRELEDYIDNLLVRVMEETPNILRIPTQVGKKAGKM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Xenopus
91
Sheep
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q6WKZ4 As Substrate

Site PTM Type Enzyme
R9 Methylation
K129 Acetylation
K131 Acetylation
T154 Phosphorylation
S156 Phosphorylation
S161 Phosphorylation
S184 Phosphorylation
T188 Phosphorylation
S190 Phosphorylation
T197 Phosphorylation
S199 Phosphorylation
S202 Phosphorylation
S206 Phosphorylation
K218 Ubiquitination
T219 Phosphorylation
S222 Phosphorylation
S224 Phosphorylation
T229 Phosphorylation
S232 Phosphorylation
S234 Phosphorylation
S236 Phosphorylation
S241 Phosphorylation
S266 Phosphorylation
S268 Phosphorylation
T278 Phosphorylation
S280 Phosphorylation
T281 Phosphorylation
T292 Phosphorylation
K296 Ubiquitination
S300 Phosphorylation
S307 Phosphorylation
S315 Phosphorylation
Y325 Phosphorylation
S338 Phosphorylation
S339 Phosphorylation
S341 Phosphorylation
S342 Phosphorylation
S343 Phosphorylation
S345 Phosphorylation
K352 Ubiquitination
S356 Phosphorylation
S357 Phosphorylation
T358 Phosphorylation
S365 Phosphorylation
S382 Phosphorylation
S384 Phosphorylation
T386 Phosphorylation
K387 Ubiquitination
S389 Phosphorylation
K391 Ubiquitination
S392 Phosphorylation
T394 Phosphorylation
S397 Phosphorylation
Y398 Phosphorylation
S431 Phosphorylation
S434 Phosphorylation
S435 Phosphorylation
S438 Phosphorylation
T441 Phosphorylation
K443 Acetylation
K448 Ubiquitination
S450 Phosphorylation
S477 Phosphorylation
S487 Phosphorylation
T491 Phosphorylation
S496 Phosphorylation
S500 Phosphorylation
S501 Phosphorylation
T511 Phosphorylation
S519 Phosphorylation
S521 Phosphorylation
S528 Phosphorylation
S529 Phosphorylation
S545 Phosphorylation
S625 Phosphorylation
T642 Phosphorylation
S660 Phosphorylation
S670 Phosphorylation
T684 Phosphorylation
S685 Phosphorylation
S686 Phosphorylation
S692 Phosphorylation
S739 Phosphorylation
S754 Phosphorylation
S758 Phosphorylation
K800 Acetylation
K803 Acetylation
S880 Phosphorylation
S925 Phosphorylation
S928 Phosphorylation
S935 Phosphorylation
S967 Phosphorylation
S1135 Phosphorylation
S1154 Phosphorylation
T1187 Phosphorylation
S1193 Phosphorylation
T1196 Phosphorylation
K1211 Ubiquitination
K1212 Ubiquitination
S1214 Phosphorylation
S1216 Phosphorylation
T1226 Phosphorylation
Y1254 Phosphorylation
T1274 Phosphorylation
K1278 Ubiquitination

Research Backgrounds

Function:

A Rab11 effector protein involved in the endosomal recycling process. Also involved in controlling membrane trafficking along the phagocytic pathway and in phagocytosis.

Subcellular Location:

Recycling endosome.
Note: Rab11A rather than Rab4A mediates localization in the endocytic recycling compartment (ERC).

Cytoplasmic vesicle>Phagosome membrane.
Note: Membrane-bound (isoform 2). Colocalizes with Rab11A at phagosomes (isoform 2).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 2 is expressed in brain, heart, testis, lung, spleen, ovary and small intestine.

Subunit Structure:

Homooligomer (isoform 2). Isoform 2 interacts with RAB4A, RAB11A, RAB11B and RAB25. According to RAB4A binding to RAB11FIP1 is of very low affinity in vitro and in vivo.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

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