PABPC4 Antibody - #DF9762

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Product: PABPC4 Antibody
Catalog: DF9762
Description: Rabbit polyclonal antibody to PABPC4
Application: WB IHC IP
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 71 kDa; 71kD(Calculated).
Uniprot: Q13310
RRID: AB_2842957

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
PABPC4 Antibody detects endogenous levels of total PABPC4.
RRID:
AB_2842957
Cite Format: Affinity Biosciences Cat# DF9762, RRID:AB_2842957.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Activated platelet protein 1; Activated-platelet protein 1; APP 1; APP-1; APP1; FLJ43938; Inducible poly(A) binding protein; Inducible poly(A)-binding protein; iPABP; IPABP4; PABP 4; PABP-4; PABP4; PABP4_HUMAN; PABPC 4; PABPC4; Poly A binding protein cytoplasmic 4; Poly(A) binding protein 4; Poly(A) binding protein cytoplasmic 4 (inducible form); Poly(A) binding protein cytoplasmic 4; Poly(A)-binding protein 4; Polyadenylate binding protein 4; Polyadenylate-binding protein 4; Polyadenylate-binding protein, cytoplasmic, 4; Polyadenylate-binding protein, inducible;

Immunogens

Immunogen:
Uniprot:

Q13310(PABP4_HUMAN) >>Visit HPA database.

Gene(ID):
PABPC4(8761)
Expression:
Q13310 PABP4_HUMAN:

Expressed at low levels in resting normal T cells; following T-cell activation, however, mRNA levels are rapidly up-regulated.

Sequence:
MNAAASSYPMASLYVGDLHSDVTEAMLYEKFSPAGPVLSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVIKGKPIRIMWSQRDPSLRKSGVGNVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGSKGYAFVHFETQEAADKAIEKMNGMLLNDRKVFVGRFKSRKEREAELGAKAKEFTNVYIKNFGEEVDDESLKELFSQFGKTLSVKVMRDPNGKSKGFGFVSYEKHEDANKAVEEMNGKEISGKIIFVGRAQKKVERQAELKRKFEQLKQERISRYQGVNLYIKNLDDTIDDEKLRKEFSPFGSITSAKVMLEDGRSKGFGFVCFSSPEEATKAVTEMNGRIVGSKPLYVALAQRKEERKAHLTNQYMQRVAGMRALPANAILNQFQPAAGGYFVPAVPQAQGRPPYYTPNQLAQMRPNPRWQQGGRPQGFQGMPSAIRQSGPRPTLRHLAPTGSECPDRLAMDFGGAGAAQQGLTDSCQSGGVPTAVQNLAPRAAVAAAAPRAVAPYKYASSVRSPHPAIQPLQAPQPAVHVQGQEPLTASMLAAAPPQEQKQMLGERLFPLIQTMHSNLAGKITGMLLEIDNSELLHMLESPESLRSKVDEAVAVLQAHHAKKEAAQKVGAVAAATS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
75
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13310 As Substrate

Site PTM Type Enzyme
S32 Phosphorylation
S39 Phosphorylation
S51 Phosphorylation
Y54 Phosphorylation
Y56 Phosphorylation
T71 Phosphorylation
K78 Ubiquitination
K80 Ubiquitination
S92 Phosphorylation
K95 Ubiquitination
S96 Phosphorylation
K104 Acetylation
K104 Ubiquitination
K108 Acetylation
K108 Ubiquitination
K113 Ubiquitination
Y116 Phosphorylation
S120 Phosphorylation
S127 Phosphorylation
K129 Ubiquitination
K138 Ubiquitination
Y140 Phosphorylation
K157 Ubiquitination
R166 Methylation
K167 Sumoylation
K167 Ubiquitination
K177 Ubiquitination
R179 Methylation
K186 Ubiquitination
K188 Methylation
K188 Ubiquitination
T191 Phosphorylation
Y194 Phosphorylation
K196 Ubiquitination
S206 Phosphorylation
K208 Ubiquitination
K216 Ubiquitination
K221 Ubiquitination
K240 Ubiquitination
K254 Ubiquitination
S257 Phosphorylation
K259 Ubiquitination
K279 Ubiquitination
K284 Ubiquitination
Y291 Phosphorylation
Y297 Phosphorylation
K299 Acetylation
K299 Ubiquitination
T304 Phosphorylation
K309 Ubiquitination
K312 Ubiquitination
S315 Phosphorylation
S319 Phosphorylation
T321 Phosphorylation
S322 Phosphorylation
K324 Ubiquitination
K333 Ubiquitination
S341 Phosphorylation
S342 Phosphorylation
K348 Ubiquitination
S360 Phosphorylation
K361 Ubiquitination
Y364 Phosphorylation
K375 Methylation
K375 Ubiquitination
T379 Phosphorylation
Y382 Phosphorylation
R385 Methylation
R419 Methylation
R432 Methylation
R436 Methylation
R442 Methylation
R454 Methylation
R459 Methylation
R463 Methylation
R509 Methylation
R518 Methylation
K524 Methylation
R530 Methylation
S531 Phosphorylation
S584 Phosphorylation
S600 Phosphorylation
S608 Phosphorylation
K629 Ubiquitination
K635 Ubiquitination
S644 Phosphorylation

Research Backgrounds

Function:

Binds the poly(A) tail of mRNA. May be involved in cytoplasmic regulatory processes of mRNA metabolism. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo (By similarity).

PTMs:

Arg-518 is dimethylated, probably to asymmetric dimethylarginine.

Subcellular Location:

Cytoplasm.
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed at low levels in resting normal T cells; following T-cell activation, however, mRNA levels are rapidly up-regulated.

Subunit Structure:

Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with NFX1. Interacts with ZFC3H1 in a RNase-sensitive manner.

Family&Domains:

Belongs to the polyadenylate-binding protein type-1 family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

· Genetic Information Processing > Translation > mRNA surveillance pathway.

· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.

Restrictive clause

 

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