PABPC3 Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using PABPC3 Antibody.

Product:	PABPC3 Antibody
Catalog:	DF9761
Description:	Rabbit polyclonal antibody to PABPC3
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Monkey
Mol.Wt.:	70 kDa; 70kD(Calculated).
Uniprot:	Q9H361
RRID:	AB_2842956

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Monkey

Clonality:

Polyclonal

Specificity:

PABPC3 Antibody detects endogenous levels of total PABPC3.

RRID:

AB_2842956
Cite Format: Affinity Biosciences Cat# DF9761, RRID:AB_2842956.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

PABP-3; PABP3; PABP3_HUMAN; PABPC3; PABPL3; Poly(A) binding protein 3; Poly(A)-binding protein 3; Polyadenylate binding protein 3; Polyadenylate-binding protein 3; RP11-165I9.5; Testis specific poly(A) binding protein; Testis-specific poly(A)-binding protein; tPABP;

Immunogens

Immunogen:

Uniprot:

Q9H361(PABP3_HUMAN) >>Visit HPA database.

Gene(ID):

PABPC3(5042)

Expression:

Q9H361 PABP3_HUMAN:

Testis specific.

Sequence:

Q9H361 PABP3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNPSTPSYPTASLYVGDLHPDVTEAMLYEKFSPAGPILSIRICRDLITSGSSNYAYVNFQHTKDAEHALDTMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIFVKNLDKSINNKALYDTVSAFGNILSCNVVCDENGSKGYGFVHFETHEAAERAIKKMNGMLLNGRKVFVGQFKSRKEREAELGARAKEFPNVYIKNFGEDMDDERLKDLFGKFGPALSVKVMTDESGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKRTFEQMKQDRITRYQVVNLYVKNLDDGIDDERLRKAFSPFGTITSAKVMMEGGRSKGFGFVCFSSPEEATKAVTEMNGRIVATKPLYVALAQRKEERQAYLTNEYMQRMASVRAVPNQRAPPSGYFMTAVPQTQNHAAYYPPSQIARLRPSPRWTAQGARPHPFQNKPSAIRPGAPRVPFSTMRPASSQVPRVMSTQRVANTSTQTVGPRPAAAAAAAATPAVRTVPRYKYAAGVRNPQQHRNAQPQVTMQQLAVHVQGQETLTASRLASAPPQKQKQMLGERLFPLIQAMHPTLAGKITGMLLEIDNSELLYMLESPESLRSKVDEAVAVLQAHQAKEATQKAVNSATGVPTV

PTMs - Q9H361 As Substrate

Q9H361

Site	PTM Type	Source
S32	Phosphorylation	Uniprot
Y56	Phosphorylation	Uniprot
T71	Phosphorylation	Uniprot
K78	Ubiquitination	Uniprot
K80	Ubiquitination	Uniprot
S92	Phosphorylation	Uniprot
K108	Ubiquitination	Uniprot
K188	Methylation	Uniprot
Y194	Phosphorylation	Uniprot
K208	Ubiquitination	Uniprot
K213	Acetylation	Uniprot
K213	Ubiquitination	Uniprot
S219	Phosphorylation	Uniprot
K221	Ubiquitination	Uniprot
T224	Phosphorylation	Uniprot
K229	Ubiquitination	Uniprot
S230	Phosphorylation	Uniprot
K231	Ubiquitination	Uniprot
S237	Phosphorylation	Uniprot
R240	Methylation	Uniprot
K246	Ubiquitination	Uniprot
K254	Ubiquitination	Uniprot
K259	Acetylation	Uniprot
K259	Ubiquitination	Uniprot
Y262	Phosphorylation	Uniprot
K269	Ubiquitination	Uniprot
K284	Ubiquitination	Uniprot
R309	Methylation	Uniprot
S315	Phosphorylation	Uniprot
K333	Ubiquitination	Uniprot
S341	Phosphorylation	Uniprot
S342	Phosphorylation	Uniprot
K348	Ubiquitination	Uniprot
T360	Phosphorylation	Uniprot
K361	Ubiquitination	Uniprot
Y364	Phosphorylation	Uniprot
R426	Methylation	Uniprot
R430	Methylation	Uniprot
R437	Methylation	Uniprot
S458	Phosphorylation	Uniprot
T459	Phosphorylation	Uniprot
R461	Methylation	Uniprot
R469	Methylation	Uniprot
S480	Phosphorylation	Uniprot
K601	Ubiquitination	Uniprot
T630	Phosphorylation	Uniprot

Research Backgrounds

Q9H361_PABP3_HUMAN

Function:

Binds the poly(A) tail of mRNA. May be involved in cytoplasmic regulatory processes of mRNA metabolism. Binds poly(A) with a slightly lower affinity as compared to PABPC1.

Subcellular Location:

Cytoplasm.

Tissue Specificity:

Testis specific.

Family&Domains:

Belongs to the polyadenylate-binding protein type-1 family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

· Genetic Information Processing > Translation > mRNA surveillance pathway.

· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.

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For Research Use Only.
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PABPC3 Antibody - #DF9761