PLXNA2 Antibody | Affinity Biosciences

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Western blot analysis of PLXNA2 using Jurkat whole cell lysates.

Product:	PLXNA2 Antibody
Catalog:	DF9760
Description:	Rabbit polyclonal antibody to PLXNA2
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	211kDa; 211kD(Calculated).
Uniprot:	O75051
RRID:	AB_2842955

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(82%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(83%)

Clonality:

Polyclonal

Specificity:

PLXNA2 Antibody detects endogenous levels of total PLXNA2.

RRID:

AB_2842955
Cite Format: Affinity Biosciences Cat# DF9760, RRID:AB_2842955.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Plexin-A2; PlexinA2; PLXA2; PLXA2_HUMAN; PLXNA2; Semaphorin receptor OCT;

Immunogens

Immunogen:

Uniprot:

O75051(PLXA2_HUMAN) >>Visit HPA database.

Gene(ID):

PLXNA2(5362)

Expression:

O75051 PLXA2_HUMAN:

Detected in fetal brain.

Sequence:

O75051 PLXA2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEQRRPWPRALEVDSRSVVLLSVVWVLLAPPAAGMPQFSTFHSENRDWTFNHLTVHQGTGAVYVGAINRVYKLTGNLTIQVAHKTGPEEDNKSCYPPLIVQPCSEVLTLTNNVNKLLIIDYSENRLLACGSLYQGVCKLLRLDDLFILVEPSHKKEHYLSSVNKTGTMYGVIVRSEGEDGKLFIGTAVDGKQDYFPTLSSRKLPRDPESSAMLDYELHSDFVSSLIKIPSDTLALVSHFDIFYIYGFASGGFVYFLTVQPETPEGVAINSAGDLFYTSRIVRLCKDDPKFHSYVSLPFGCTRAGVEYRLLQAAYLAKPGDSLAQAFNITSQDDVLFAIFSKGQKQYHHPPDDSALCAFPIRAINLQIKERLQSCYQGEGNLELNWLLGKDVQCTKAPVPIDDNFCGLDINQPLGGSTPVEGLTLYTTSRDRMTSVASYVYNGYSVVFVGTKSGKLKKIRADGPPHGGVQYEMVSVLKDGSPILRDMAFSIDQRYLYVMSERQVTRVPVESCEQYTTCGECLSSGDPHCGWCALHNMCSRRDKCQQAWEPNRFAASISQCVSLAVHPSSISVSEHSRLLSLVVSDAPDLSAGIACAFGNLTEVEGQVSGSQVICISPGPKDVPVIPLDQDWFGLELQLRSKETGKIFVSTEFKFYNCSAHQLCLSCVNSAFRCHWCKYRNLCTHDPTTCSFQEGRINISEDCPQLVPTEEILIPVGEVKPITLKARNLPQPQSGQRGYECVLNIQGAIHRVPALRFNSSSVQCQNSSYQYDGMDISNLAVDFAVVWNGNFIIDNPQDLKVHLYKCAAQRESCGLCLKADRKFECGWCSGERRCTLHQHCTSPSSPWLDWSSHNVKCSNPQITEILTVSGPPEGGTRVTIHGVNLGLDFSEIAHHVQVAGVPCTPLPGEYIIAEQIVCEMGHALVGTTSGPVRLCIGECKPEFMTKSHQQYTFVNPSVLSLNPIRGPESGGTMVTITGHYLGAGSSVAVYLGNQTCEFYGRSMSEIVCVSPPSSNGLGPVPVSVSVDRAHVDSNLQFEYIDDPRVQRIEPEWSIASGHTPLTITGFNLDVIQEPRIRVKFNGKESVNVCKVVNTTTLTCLAPSLTTDYRPGLDTVERPDEFGFVFNNVQSLLIYNDTKFIYYPNPTFELLSPTGVLDQKPGSPIILKGKNLCPPASGGAKLNYTVLIGETPCAVTVSETQLLCEPPNLTGQHKVMVHVGGMVFSPGSVSVISDSLLTLPAIVSIAAGGSLLLIIVIIVLIAYKRKSRENDLTLKRLQMQMDNLESRVALECKEAFAELQTDINELTSDLDRSGIPYLDYRTYAMRVLFPGIEDHPVLRELEVQGNGQQHVEKALKLFAQLINNKVFLLTFIRTLELQRSFSMRDRGNVASLIMTGLQGRLEYATDVLKQLLSDLIDKNLENKNHPKLLLRRTESVAEKMLTNWFAFLLHKFLKECAGEPLFMLYCAIKQQMEKGPIDAITGEARYSLSEDKLIRQQIEYKTLILNCVNPDNENSPEIPVKVLNCDTITQVKEKILDAVYKNVPYSQRPRAVDMDLEWRQGRIARVVLQDEDITTKIEGDWKRLNTLMHYQVSDRSVVALVPKQTSSYNIPASASISRTSISRYDSSFRYTGSPDSLRSRAPMITPDLESGVKVWHLVKNHDHGDQKEGDRGSKMVSEIYLTRLLATKGTLQKFVDDLFETLFSTVHRGSALPLAIKYMFDFLDEQADRHSIHDTDVRHTWKSNCLPLRFWVNVIKNPQFVFDIHKGSITDACLSVVAQTFMDSCSTSEHRLGKDSPSNKLLYAKDIPSYKSWVERYYADIAKLPAISDQDMNAYLAEQSRLHAVEFNMLSALNEIYSYVSKYSEELIGALEQDEQARRQRLAYKVEQLINAMSIES

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Dog

100

Rabbit

100

Sheep

Chicken

Zebrafish

Horse

Bovine

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75051 As Substrate

O75051

Site	PTM Type	Source
	Phosphorylation	Uniprot
S15	Phosphorylation	Uniprot
K138	Acetylation	Uniprot
K154	Acetylation	Uniprot
Y293	Phosphorylation	Uniprot
S295	Phosphorylation	Uniprot
S434	Phosphorylation	Uniprot
S444	Phosphorylation	Uniprot
Y470	Phosphorylation	Uniprot
S474	Phosphorylation	Uniprot
S850	Phosphorylation	Uniprot
S1264	Phosphorylation	Uniprot
K1272	Ubiquitination	Uniprot
S1388	Phosphorylation	Uniprot
K1424	Ubiquitination	Uniprot
K1451	Acetylation	Uniprot
K1489	Ubiquitination	Uniprot
T1524	Phosphorylation	Uniprot
Y1605	Phosphorylation	Uniprot
S1612	Phosphorylation	Uniprot
Y1627	Phosphorylation	Uniprot
T1628	Phosphorylation	Uniprot
S1630	Phosphorylation	Uniprot
S1633	Phosphorylation	Uniprot
S1636	Phosphorylation	Uniprot
S1670	Phosphorylation	Uniprot
Y1677	Phosphorylation	Uniprot
K1685	Ubiquitination	Uniprot
K1791	Ubiquitination	Uniprot
K1797	Ubiquitination	Uniprot
Y1800	Phosphorylation	Uniprot
S1806	Phosphorylation	Uniprot
Y1807	Phosphorylation	Uniprot
Y1814	Phosphorylation	Uniprot
Y1815	Phosphorylation	Uniprot
S1825	Phosphorylation	Uniprot
Y1860	Phosphorylation	Uniprot
S1861	Phosphorylation	Uniprot

Research Backgrounds

O75051_PLXA2_HUMAN

Function:

Coreceptor for SEMA3A and SEMA6A. Necessary for signaling by SEMA6A and class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm (By similarity).

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Tissue Specificity:

Detected in fetal brain.

Subunit Structure:

Homodimer. The PLXNA2 homodimer interacts with a SEMA6A homodimer, giving rise to a heterotetramer. Interacts directly with NRP1 and NRP2 (By similarity). Interacts with RND1.

Family&Domains:

Belongs to the plexin family.

Research Fields

· Organismal Systems > Development > Axon guidance. (View pathway)

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PLXNA2 Antibody - #DF9760