ATP2B2 Antibody - #DF9757

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Product: ATP2B2 Antibody
Catalog: DF9757
Description: Rabbit polyclonal antibody to ATP2B2
Application: WB IHC IF/ICC
Reactivity: Human, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 137 kDa; 137kD(Calculated).
Uniprot: Q01814
RRID: AB_2842952

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
ATP2B2 Antibody detects endogenous levels of total ATP2B2.
RRID:
AB_2842952
Cite Format: Affinity Biosciences Cat# DF9757, RRID:AB_2842952.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AT2B2_HUMAN; atp2b2; ATPase plasma membrane Ca2+ transporting 2; Plasma membrane calcium ATPase 2; Plasma membrane calcium ATPase; Plasma membrane calcium ATPase isoform 2; Plasma membrane calcium pump isoform 2; Plasma membrane calcium transporting ATPase 2; Plasma membrane calcium-transporting ATPase 2; PMCA2; PMCA2a; PMCA2i;

Immunogens

Immunogen:
Uniprot:

Q01814(AT2B2_HUMAN) >>Visit HPA database.

Gene(ID):
ATP2B2(491)
Expression:
Q01814 AT2B2_HUMAN:

Mainly expressed in brain cortex. Found in low levels in skeletal muscle, heart muscle, stomach, liver, kidney and lung. Isoforms containing segment B are found in brain cortex and at low levels in other tissues. Isoforms containing segments X and W are found at low levels in all tissues. Isoforms containing segment A and segment Z are found at low levels in skeletal muscle and heart muscle.

Sequence:
MGDMTNSDFYSKNQRNESSHGGEFGCTMEELRSLMELRGTEAVVKIKETYGDTEAICRRLKTSPVEGLPGTAPDLEKRKQIFGQNFIPPKKPKTFLQLVWEALQDVTLIILEIAAIISLGLSFYHPPGEGNEGCATAQGGAEDEGEAEAGWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVVRAGQVVQIPVAEIVVGDIAQVKYGDLLPADGLFIQGNDLKIDESSLTGESDQVRKSVDKDPMLLSGTHVMEGSGRMLVTAVGVNSQTGIIFTLLGAGGEEEEKKDKKGVKKGDGLQLPAADGAAASNAADSANASLVNGKMQDGNVDASQSKAKQQDGAAAMEMQPLKSAEGGDADDRKKASMHKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILVLYFTVDTFVVNKKPWLPECTPVYVQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQAYVGDVHYKEIPDPSSINTKTMELLINAIAINSAYTTKILPPEKEGALPRQVGNKTECGLLGFVLDLKQDYEPVRSQMPEEKLYKVYTFNSVRKSMSTVIKLPDESFRMYSKGASEIVLKKCCKILNGAGEPRVFRPRDRDEMVKKVIEPMACDGLRTICVAYRDFPSSPEPDWDNENDILNELTCICVVGIEDPVRPEVPEAIRKCQRAGITVRMVTGDNINTARAIAIKCGIIHPGEDFLCLEGKEFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTHTEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTETLLLRKPYGRNKPLISRTMMKNILGHAVYQLALIFTLLFVGEKMFQIDSGRNAPLHSPPSEHYTIIFNTFVMMQLFNEINARKIHGERNVFDGIFRNPIFCTIVLGTFAIQIVIVQFGGKPFSCSPLQLDQWMWCIFIGLGELVWGQVIATIPTSRLKFLKEAGRLTQKEEIPEEELNEDVEEIDHAERELRRGQILWFRGLNRIQTQIRVVKAFRSSLYEGLEKPESRTSIHNFMAHPEFRIEDSQPHIPLIDDTDLEEDAALKQNSSPPSSLNKNNSAIDSGINLTTDTSKSATSSSPGSPIHSLETSL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
73
Pig
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q01814 As Substrate

Site PTM Type Enzyme
T5 Phosphorylation
S18 Phosphorylation
K177 Ubiquitination
S184 Phosphorylation
K301 Acetylation
S346 Phosphorylation
K383 Ubiquitination
K389 Acetylation
K389 Ubiquitination
T449 Phosphorylation
Y469 Phosphorylation
S470 Phosphorylation
K476 Ubiquitination
S498 Phosphorylation
S528 Phosphorylation
Y597 Phosphorylation
S604 Phosphorylation
T726 Phosphorylation
K770 Ubiquitination
K780 Ubiquitination
S792 Phosphorylation
T794 Phosphorylation
K796 Ubiquitination
T798 Phosphorylation
T818 Phosphorylation
T822 Phosphorylation
K829 Ubiquitination
Y940 Phosphorylation
K944 Ubiquitination
R1097 Methylation
T1139 Phosphorylation
S1149 Phosphorylation
S1150 Phosphorylation
Y1152 Phosphorylation
K1157 Ubiquitination
S1160 Phosphorylation
T1162 Phosphorylation
S1178 Phosphorylation
S1211 Phosphorylation
S1229 Phosphorylation
S1230 Phosphorylation
S1231 Phosphorylation
S1234 Phosphorylation
S1238 Phosphorylation
T1241 Phosphorylation
S1242 Phosphorylation

Research Backgrounds

Function:

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.

Subcellular Location:

Cell junction>Synapse. Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Mainly expressed in brain cortex. Found in low levels in skeletal muscle, heart muscle, stomach, liver, kidney and lung. Isoforms containing segment B are found in brain cortex and at low levels in other tissues. Isoforms containing segments X and W are found at low levels in all tissues. Isoforms containing segment A and segment Z are found at low levels in skeletal muscle and heart muscle.

Subunit Structure:

Interacts with PDZD11.

Family&Domains:

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Digestive system > Salivary secretion.

· Organismal Systems > Digestive system > Pancreatic secretion.

References

1). Investigation of the underlying mechanism of Buyang Huanwu decoction in ischemic stroke by integrating systems pharmacology-proteomics and in vivo experiments. Fitoterapia, 2024 (PubMed: 38580032) [IF=3.4]

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