PLD3 Antibody - #DF9753
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF9753, RRID:AB_2842948.
Fold/Unfold
Choline phosphatase 3; HindIII K4L homolog; HU K4; Hu-K4; HUK4; Phosphatidylcholine hydrolyzing phospholipase D3; Phosphatidylcholine-hydrolyzing phospholipase D3; Phospholipase D family, member 3; Phospholipase D3; PLD 3; PLD3; PLD3_HUMAN;
Immunogens
Widely expressed. In the brain, high levels of expression are detected in the frontal, temporal and occipital cortices and hippocampus. Expressed at low level in corpus callosum.
- Q8IV08 PLD3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKPKLMYQELKVPAEEPANELPMNEIEAWKAAEKKARWVLLVLILAVVGFGALMTQLFLWEYGDLHLFGPNQRPAPCYDPCEAVLVESIPEGLDFPNASTGNPSTSQAWLGLLAGAHSSLDIASFYWTLTNNDTHTQEPSAQQGEEVLRQLQTLAPKGVNVRIAVSKPSGPQPQADLQALLQSGAQVRMVDMQKLTHGVLHTKFWVVDQTHFYLGSANMDWRSLTQVKELGVVMYNCSCLARDLTKIFEAYWFLGQAGSSIPSTWPRFYDTRYNQETPMEICLNGTPALAYLASAPPPLCPSGRTPDLKALLNVVDNARSFIYVAVMNYLPTLEFSHPHRFWPAIDDGLRRATYERGVKVRLLISCWGHSEPSMRAFLLSLAALRDNHTHSDIQVKLFVVPADEAQARIPYARVNHNKYMVTERATYIGTSNWSGNYFTETAGTSLLVTQNGRGGLRSQLEAIFLRDWDSPYSHDLDTSADSVGNACRLL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8IV08 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K4 | Ubiquitination | Uniprot | |
| Y7 | Phosphorylation | Uniprot | |
| K11 | Ubiquitination | Uniprot | |
| K30 | Ubiquitination | Uniprot | |
| N97 | N-Glycosylation | Uniprot | |
| N132 | N-Glycosylation | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| S223 | Phosphorylation | Uniprot | |
| T225 | Phosphorylation | Uniprot | |
| S238 | Phosphorylation | Uniprot | |
| K309 | Ubiquitination | Uniprot |
Research Backgrounds
5'->3' DNA exonuclease which digests single-stranded DNA (ssDNA). Regulates inflammatory cytokine responses via the degradation of nucleic acids, by reducing the concentration of ssDNA able to stimulate TLR9, a nucleotide-sensing receptor in collaboration with PLD4 (By similarity). May be important in myotube formation. Plays a role in lysosomal homeostasis. Involved in the regulation of endosomal protein sorting.
N-glycosylated.
Proteolytically processed to a soluble form that is stable within endosomes and lysosomes. During transport through the secretory pathway becomes proteolysed by cysteine proteases, thereby releasing a stable soluble lysosomal lumenal polypeptide, whereas the transmembrane-bound fragment is rapidly degraded. Its transport route to lysosomes involves ubiquitination and the ESCRT complex.
Ubiquitinated at N-terminus. Ubiquitination mediates sorting into lysosomes.
Endoplasmic reticulum membrane>Single-pass type II membrane protein. Lysosome lumen. Early endosome membrane>Single-pass type II membrane protein. Late endosome membrane>Single-pass type II membrane protein. Golgi apparatus membrane>Single-pass type II membrane protein. Endosome membrane>Single-pass type II membrane protein.
Note: Localizes to ER-associated vesicles in differentiating myotubes (PubMed:22428023). The soluble form in lysosome arises by proteolytic processing of the membrane-bound form (PubMed:29386126). Colocalizes with APP in endosomes (PubMed:29368044).
Widely expressed. In the brain, high levels of expression are detected in the frontal, temporal and occipital cortices and hippocampus. Expressed at low level in corpus callosum.
Interacts with APP.
Belongs to the phospholipase D family.
Research Fields
· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.
· Metabolism > Lipid metabolism > Ether lipid metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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