Product: PIGA Antibody
Catalog: DF9742
Description: Rabbit polyclonal antibody to PIGA
Application: WB
Reactivity: Human, Mouse, Rat, Monkey
Mol.Wt.: 54 kDa; 54kD(Calculated).
Uniprot: P37287
RRID: AB_2842937

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Clonality:
Polyclonal
Specificity:
PIGA Antibody detects endogenous levels of total PIGA.
RRID:
AB_2842937
Cite Format: Affinity Biosciences Cat# DF9742, RRID:AB_2842937.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Class A GlcNAc inositol phospholipid assembly protein; GlcNAc PI synthesis protein; GlcNAc-PI synthesis protein; GPI3; MCAHS2; Phosphatidylinositol glycan anchor biosynthesis class A; Phosphatidylinositol glycan biosynthesis class A protein; Phosphatidylinositol N-acetylglucosaminyltransferase subunit A; Phosphatidylinositol-glycan biosynthesis class A protein; PIG A; PIG-A; Piga; PIGA_HUMAN;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MACRGGAGNGHRASATLSRVSPGSLYTCRTRTHNICMVSDFFYPNMGGVESHIYQLSQCLIERGHKVIIVTHAYGNRKGIRYLTSGLKVYYLPLKVMYNQSTATTLFHSLPLLRYIFVRERVTIIHSHSSFSAMAHDALFHAKTMGLQTVFTDHSLFGFADVSSVLTNKLLTVSLCDTNHIICVSYTSKENTVLRAALNPEIVSVIPNAVDPTDFTPDPFRRHDSITIVVVSRLVYRKGIDLLSGIIPELCQKYPDLNFIIGGEGPKRIILEEVRERYQLHDRVRLLGALEHKDVRNVLVQGHIFLNTSLTEAFCMAIVEAASCGLQVVSTRVGGIPEVLPENLIILCEPSVKSLCEGLEKAIFQLKSGTLPAPENIHNIVKTFYTWRNVAERTEKVYDRVSVEAVLPMDKRLDRLISHCGPVTGYIFALLAVFNFLFLIFLRWMTPDSIIDVAIDATGPRGAWTNNYSHSKRGGENNEISETR

PTMs - P37287 As Substrate

Site PTM Type Enzyme
R4 Methylation
S21 Phosphorylation
S24 Phosphorylation
T27 Phosphorylation
T32 Phosphorylation
Y254 Phosphorylation
K293 Ubiquitination
S354 Phosphorylation
K361 Ubiquitination
K367 Ubiquitination
Y385 Phosphorylation
K396 Acetylation

Research Backgrounds

Function:

Necessary for the synthesis of N-acetylglucosaminyl-phosphatidylinositol, the very early intermediate in GPI-anchor biosynthesis.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Associates with PIGC, PIGH, PIGP, PIGQ and DPM2. The latter is not essential for activity. Interacts directly with PIGY.

Family&Domains:

Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.

Research Fields

· Metabolism > Glycan biosynthesis and metabolism > Glycosylphosphatidylinositol (GPI)-anchor biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.