HSD17B4 Antibody - #DF9735

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Product: HSD17B4 Antibody
Catalog: DF9735
Description: Rabbit polyclonal antibody to HSD17B4
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 80 kDa; 80kD(Calculated).
Uniprot: P51659
RRID: AB_2842930

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 100ul $280 In stock
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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
HSD17B4 Antibody detects endogenous levels of total HSD17B4.
RRID:
AB_2842930
Cite Format: Affinity Biosciences Cat# DF9735, RRID:AB_2842930.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase; 17 beta HSD 4; 17 beta HSD IV; 17 beta hydroxysteroid dehydrogenase 4; 17-beta-HSD 4; 17-beta-hydroxysteroid dehydrogenase 4; 17beta estradiol dehydrogenase type IV; 3 alpha 7 alpha12 alpha trihydroxy 5 beta cholest 24 enoyl CoA hydratase; 3-alpha; 7-alpha; Beta hydroxyacyl dehydrogenase; Beta keto reductase; D 3 hydroxyacyl CoA dehydratase; D bifunctional protein; D bifunctional protein peroxisomal; D-3-hydroxyacyl CoA dehydratase/D-3-hydroxyacyl-CoA dehydrogenase bifunctional protein; D-bifunctional protein; D-bifunctional protein, peroxisomal; DBP; DBP, peroxisomal; DHB4_HUMAN; EDH17B4; Enoyl-CoA hydratase 2; Hsd17b4; MFE 2; MFE-2; MPF-2; Multifunctional protein 2; Peroxisomal multifunctional enzyme type 2; Peroxisomal multifunctional protein 2; PRLTS1; SDR8C1; Short chain dehydrogenase/reductase family 8C member 1;

Immunogens

Immunogen:
Uniprot:

P51659(DHB4_HUMAN) >>Visit HPA database.

Gene(ID):
HSD17B4(3295)
Expression:
P51659 DHB4_HUMAN:

Present in many tissues with highest concentrations in liver, heart, prostate and testis.

Sequence:
MGSPLRFDGRVVLVTGAGAGLGRAYALAFAERGALVVVNDLGGDFKGVGKGSLAADKVVEEIRRRGGKAVANYDSVEEGEKVVKTALDAFGRIDVVVNNAGILRDRSFARISDEDWDIIHRVHLRGSFQVTRAAWEHMKKQKYGRIIMTSSASGIYGNFGQANYSAAKLGLLGLANSLAIEGRKSNIHCNTIAPNAGSRMTQTVMPEDLVEALKPEYVAPLVLWLCHESCEENGGLFEVGAGWIGKLRWERTLGAIVRQKNHPMTPEAVKANWKKICDFENASKPQSIQESTGSIIEVLSKIDSEGGVSANHTSRATSTATSGFAGAIGQKLPPFSYAYTELEAIMYALGVGASIKDPKDLKFIYEGSSDFSCLPTFGVIIGQKSMMGGGLAEIPGLSINFAKVLHGEQYLELYKPLPRAGKLKCEAVVADVLDKGSGVVIIMDVYSYSEKELICHNQFSLFLVGSGGFGGKRTSDKVKVAVAIPNRPPDAVLTDTTSLNQAALYRLSGDWNPLHIDPNFASLAGFDKPILHGLCTFGFSARRVLQQFADNDVSRFKAIKARFAKPVYPGQTLQTEMWKEGNRIHFQTKVQETGDIVISNAYVDLAPTSGTSAKTPSEGGKLQSTFVFEEIGRRLKDIGPEVVKKVNAVFEWHITKGGNIGAKWTIDLKSGSGKVYQGPAKGAADTTIILSDEDFMEVVLGKLDPQKAFFSGRLKARGNIMLSQKLQMILKDYAKL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P51659 As Substrate

Site PTM Type Enzyme
S3 Phosphorylation
T15 Phosphorylation
K46 Acetylation
K50 Ubiquitination
S52 Phosphorylation
K57 Acetylation
K57 Ubiquitination
K68 Ubiquitination
S75 Phosphorylation
K81 Acetylation
K81 Ubiquitination
S112 Phosphorylation
K139 Acetylation
Y143 Phosphorylation
T149 Phosphorylation
S150 Phosphorylation
S151 Phosphorylation
Y156 Phosphorylation
K184 Ubiquitination
S185 Phosphorylation
T265 Phosphorylation
K270 Acetylation
K270 Ubiquitination
K275 Ubiquitination
S294 Phosphorylation
S300 Phosphorylation
S304 Phosphorylation
S309 Phosphorylation
T313 Phosphorylation
T317 Phosphorylation
S318 Phosphorylation
T319 Phosphorylation
S322 Phosphorylation
Y347 Phosphorylation
Y414 Phosphorylation
K415 Acetylation
K415 Ubiquitination
K424 Ubiquitination
K565 Acetylation
K565 Ubiquitination
K579 Acetylation
Y602 Phosphorylation
K621 Acetylation
K636 Acetylation
K636 Ubiquitination
K644 Acetylation
T655 Phosphorylation
K669 Acetylation
K674 Ubiquitination
K707 Acetylation
K707 Ubiquitination
S711 Phosphorylation
K731 Acetylation
K731 Ubiquitination

Research Backgrounds

Function:

Bifunctional enzyme acting on the peroxisomal beta-oxidation pathway for fatty acids. Catalyzes the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids.

Subcellular Location:

Peroxisome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Present in many tissues with highest concentrations in liver, heart, prostate and testis.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Research Fields

· Cellular Processes > Transport and catabolism > Peroxisome.   (View pathway)

· Metabolism > Lipid metabolism > Primary bile acid biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

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