P2RX1 Antibody | Affinity Biosciences

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Product:	P2RX1 Antibody
Catalog:	DF9728
Description:	Rabbit polyclonal antibody to P2RX1
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	45 kDa; 45kD(Calculated).
Uniprot:	P51575
RRID:	AB_2842923

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(92%), Bovine(83%), Horse(100%), Sheep(83%), Rabbit(92%), Dog(100%)

Clonality:

Polyclonal

Specificity:

P2RX1 Antibody detects endogenous levels of total P2RX1.

RRID:

AB_2842923
Cite Format: Affinity Biosciences Cat# DF9728, RRID:AB_2842923.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ATP receptor; H.sapiens mRNA for ATP receptor; P2 RX1; P2rx1; P2RX1 protein; P2RX1_HUMAN; P2X purinoceptor 1; P2X receptor subunit 1; P2X1; P2X1 receptor; Purinergic receptor; Purinergic receptor P2X ligand gated ion channel 1; Purinergic receptor P2X1;

Immunogens

Immunogen:

Uniprot:

P51575(P2RX1_HUMAN) >>Visit HPA database.

Gene(ID):

P2RX1(5023)

Sequence:

P51575 P2RX1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MARRFQEELAAFLFEYDTPRMVLVRNKKVGVIFRLIQLVVLVYVIGWVFLYEKGYQTSSGLISSVSVKLKGLAVTQLPGLGPQVWDVADYVFPAQGDNSFVVMTNFIVTPKQTQGYCAEHPEGGICKEDSGCTPGKAKRKAQGIRTGKCVAFNDTVKTCEIFGWCPVEVDDDIPRPALLREAENFTLFIKNSISFPRFKVNRRNLVEEVNAAHMKTCLFHKTLHPLCPVFQLGYVVQESGQNFSTLAEKGGVVGITIDWHCDLDWHVRHCRPIYEFHGLYEEKNLSPGFNFRFARHFVENGTNYRHLFKVFGIRFDILVDGKAGKFDIIPTMTTIGSGIGIFGVATVLCDLLLLHILPKRHYYKQKKFKYAEDMGPGAAERDLAATSSTLGLQENMRTS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Dog

100

Pig

Rabbit

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P51575 As Substrate

P51575

Site	PTM Type	Source
Y16	Phosphorylation	Uniprot
T18	Phosphorylation	Uniprot
S130	Phosphorylation	Uniprot
K138	Acetylation	Uniprot
Y274	Phosphorylation	Uniprot
S286	Phosphorylation	Uniprot
Y370	Phosphorylation	Uniprot
T386	Phosphorylation	Uniprot
S387	Phosphorylation	Uniprot
S388	Phosphorylation	Uniprot
T389	Phosphorylation	Uniprot
T398	Phosphorylation	Uniprot
S399	Phosphorylation	Uniprot

Research Backgrounds

P51575_P2RX1_HUMAN

Function:

Ligand-gated ion channel with relatively high calcium permeability. Binding to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle. Seems to be linked to apoptosis, by increasing the intracellular concentration of calcium in the presence of ATP, leading to programmed cell death (By similarity).

Subcellular Location:

Membrane>Multi-pass membrane protein.

Subunit Structure:

Homo- or heteropolymers.

Family&Domains:

Belongs to the P2X receptor family.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Immune system > Platelet activation. (View pathway)

References

1). NcRNAs-mediated P2RX1 expression correlates with clinical outcomes and immune infiltration in patients with breast invasive carcinoma. Imaging, 2022 (PubMed: 35585027) [IF=0.4]

Application: WB Species: Human Sample: breast cancer cell

Figure 2. P2RX1 expression in normal human mammary epithelial cell line and two human breast cancer cell lines. (A) Analysis of P2RX1 expression by RT-qPCR. (B) Analysis of P2RX1 expression by Western blot.

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For Research Use Only.
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P2RX1 Antibody - #DF9728