NVL Antibody - #DF9714

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Product: NVL Antibody
Catalog: DF9714
Description: Rabbit polyclonal antibody to NVL
Application: WB IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 95 kDa; 95kD(Calculated).
Uniprot: O15381
RRID: AB_2842909

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MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
NVL Antibody detects endogenous levels of total NVL.
RRID:
AB_2842909
Cite Format: Affinity Biosciences Cat# DF9714, RRID:AB_2842909.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Nuclear valosin containing protein like; Nuclear valosin-containing protein-like; Nuclear VCP like; Nuclear VCP-like protein; Nvl; NVL_HUMAN; NVLp; OTTHUMP00000035641; OTTHUMP00000216593; OTTHUMP00000216594; OTTHUMP00000216599; OTTHUMP00000216600;

Immunogens

Immunogen:
Uniprot:

O15381(NVL_HUMAN) >>Visit HPA database.

Gene(ID):
NVL(4931)
Expression:
O15381 NVL_HUMAN:

Widely expressed. Highest level of expression in heart, placenta, skeletal muscle, pancreas and retina.

Sequence:
MKPRPAGFVDNKLKQRVIQYLTSNKCGKYVDIGVLASDLQRVYSIDYGRRKRNAFRIQVEKVFSIISSEKELKNLTELEDEHLAKRARQGEEDNEYTESYSDDDSSMEDYPDPQSANHMNSSLLSLYRKGNPDSVSNTPEMEQRETTSSTPRISSKTGSIPLKTPAKDSEGGWFIDKTPSVKKDSFFLDLSCEKSNPKKPITEIQDSKDSSLLESDMKRKGKLKNKGSKRKKEDLQEVDGEIEAVLQKKAKARGLEFQISNVKFEDVGGNDMTLKEVCKMLIHMRHPEVYHHLGVVPPRGVLLHGPPGCGKTLLAHAIAGELDLPILKVAAPEIVSGVSGESEQKLRELFEQAVSNAPCIIFIDEIDAITPKREVASKDMERRIVAQLLTCMDDLNNVAATARVLVIGATNRPDSLDPALRRAGRFDREICLGIPDEASRERILQTLCRKLRLPQAFDFCHLAHLTPGFVGADLMALCREAAMCAVNRVLMKLQEQQKKNPEMEDLPSKGVQEERLGTEPTSETQDELQRLLGLLRDQDPLSEEQMQGLCIELNDFIVALSSVQPSAKREGFVTVPNVTWADIGALEDIREELTMAILAPVRNPDQFKALGLVTPAGVLLAGPPGCGKTLLAKAVANESGLNFISVKGPELLNMYVGESERAVRQVFQRAKNSAPCVIFFDEVDALCPRRSDRETGASVRVVNQLLTEMDGLEARQQVFIMAATNRPDIIDPAILRPGRLDKTLFVGLPPPADRLAILKTITKNGTKPPLDADVNLEAIAGDLRCDCYTGADLSALVREASICALRQEMARQKSGNEKGELKVSHKHFEEAFKKVRSSISKKDQIMYERLQESLSR

PTMs - O15381 As Substrate

Site PTM Type Enzyme
K12 Ubiquitination
S23 Phosphorylation
K25 Ubiquitination
Y29 Phosphorylation
S37 Phosphorylation
S44 Phosphorylation
K70 Acetylation
K70 Ubiquitination
K73 Ubiquitination
T76 Phosphorylation
K85 Ubiquitination
K129 Acetylation
K129 Ubiquitination
S134 Phosphorylation
T138 Phosphorylation
T147 Phosphorylation
S149 Phosphorylation
T150 Phosphorylation
K156 Ubiquitination
S159 Phosphorylation
K163 Ubiquitination
T164 Phosphorylation
K167 Ubiquitination
K177 Ubiquitination
T178 Phosphorylation
S180 Phosphorylation
S185 Phosphorylation
S191 Phosphorylation
K194 Ubiquitination
S195 Phosphorylation
K199 Ubiquitination
S207 Phosphorylation
K208 Sumoylation
S210 Phosphorylation
S211 Phosphorylation
S215 Phosphorylation
Y290 Phosphorylation
K345 Ubiquitination
S355 Phosphorylation
K492 Ubiquitination
K498 Acetylation
K499 Ubiquitination
K509 Ubiquitination
Y655 Phosphorylation
K671 Ubiquitination
K759 Ubiquitination
S801 Phosphorylation
S814 Phosphorylation
K826 Ubiquitination
S837 Phosphorylation
S838 Phosphorylation
S840 Phosphorylation

Research Backgrounds

Function:

Participates in the assembly of the telomerase holoenzyme and effecting of telomerase activity via its interaction with TERT. Involved in both early and late stages of the pre-rRNA processing pathways. Spatiotemporally regulates 60S ribosomal subunit biogenesis in the nucleolus. Catalyzes the release of specific assembly factors, such as WDR74, from pre-60S ribosomal particles through the ATPase activity.

Subcellular Location:

Nucleus>Nucleoplasm.

Nucleus>Nucleolus. Nucleus>Nucleoplasm.
Note: Expressed predominantly in the nucleolus. Associates with pre-ribosomal particles in the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Highest level of expression in heart, placenta, skeletal muscle, pancreas and retina.

Subunit Structure:

Interacts with NCL/nucleolin. Isoform 1 and isoform 2 interact with TERT and isoform 1 exhibits a higher binding affinity for TERT compared to isoform 2. Isoform 1 interacts with MTREX in an ATP-dependent manner; the interaction is required to associate NVL with nuclear RNA exosome. Isoform 1 interacts with RPL5 in an ATP-dependent manner. Interacts with WDR74 (through WDR repeats); the interaction is independent of RNA or pre-60S ribosome particles.

Family&Domains:

Belongs to the AAA ATPase family.

Research Fields

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

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