NUP214 Antibody - #DF9710

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Product: NUP214 Antibody
Catalog: DF9710
Description: Rabbit polyclonal antibody to NUP214
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 214kDa; 214kD(Calculated).
Uniprot: P35658
RRID: AB_2842905

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MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%)
Clonality:
Polyclonal
Specificity:
NUP214 Antibody detects endogenous levels of total NUP214.
RRID:
AB_2842905
Cite Format: Affinity Biosciences Cat# DF9710, RRID:AB_2842905.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

214 kDa nucleoporin; CAIN; CAN; CAN protein putative oncogene; D9S46E; N214; NU214_HUMAN; Nuclear pore complex protein Nup214; nucleoporin 214kDa; Nucleoporin Nup214; Nup214; p250; Protein CAN;

Immunogens

Immunogen:
Uniprot:

P35658(NU214_HUMAN) >>Visit HPA database.

Gene(ID):
NUP214(8021)
Expression:
P35658 NU214_HUMAN:

Expressed in thymus, spleen, bone marrow, kidney, brain and testis, but hardly in all other tissues or in whole embryos during development.

Sequence:
MGDEMDAMIPEREMKDFQFRALKKVRIFDSPEELPKERSSLLAVSNKYGLVFAGGASGLQIFPTKNLLIQNKPGDDPNKIVDKVQGLLVPMKFPIHHLALSCDNLTLSACMMSSEYGSIIAFFDVRTFSNEAKQQKRPFAYHKLLKDAGGMVIDMKWNPTVPSMVAVCLADGSIAVLQVTETVKVCATLPSTVAVTSVCWSPKGKQLAVGKQNGTVVQYLPTLQEKKVIPCPPFYESDHPVRVLDVLWIGTYVFAIVYAAADGTLETSPDVVMALLPKKEEKHPEIFVNFMEPCYGSCTERQHHYYLSYIEEWDLVLAASAASTEVSILARQSDQINWESWLLEDSSRAELPVTDKSDDSLPMGVVVDYTNQVEITISDEKTLPPAPVLMLLSTDGVLCPFYMINQNPGVKSLIKTPERLSLEGERQPKSPGSTPTTPTSSQAPQKLDASAAAAPASLPPSSPAAPIATFSLLPAGGAPTVFSFGSSSLKSSATVTGEPPSYSSGSDSSKAAPGPGPSTFSFVPPSKASLAPTPAASPVAPSAASFSFGSSGFKPTLESTPVPSVSAPNIAMKPSFPPSTSAVKVNLSEKFTAAATSTPVSSSQSAPPMSPFSSASKPAASGPLSHPTPLSAPPSSVPLKSSVLPSPSGRSAQGSSSPVPSMVQKSPRITPPAAKPGSPQAKSLQPAVAEKQGHQWKDSDPVMAGIGEEIAHFQKELEELKARTSKACFQVGTSEEMKMLRTESDDLHTFLLEIKETTESLHGDISSLKTTLLEGFAGVEEAREQNERNRDSGYLHLLYKRPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDQHLEQKKKQRHLLVPERETLFNTLANNREIINQQRKRLNHLVDSLQQLRLYKQTSLWSLSSAVPSQSSIHSFDSDLESLCNALLKTTIESHTKSLPKVPAKLSPMKQAQLRNFLAKRKTPPVRSTAPASLSRSAFLSQRYYEDLDEVSSTSSVSQSLESEDARTSCKDDEAVVQAPRHAPVVRTPSIQPSLLPHAAPFAKSHLVHGSSPGVMGTSVATSASKIIPQGADSTMLATKTVKHGAPSPSHPISAPQAAAAAALRRQMASQAPAVNTLTESTLKNVPQVVNVQELKNNPATPSTAMGSSVPYSTAKTPHPVLTPVAANQAKQGSLINSLKPSGPTPASGQLSSGDKASGTAKIETAVTSTPSASGQFSKPFSFSPSGTGFNFGIITPTPSSNFTAAQGATPSTKESSQPDAFSSGGGSKPSYEAIPESSPPSGITSASNTTPGEPAASSSRPVAPSGTALSTTSSKLETPPSKLGELLFPSSLAGETLGSFSGLRVGQADDSTKPTNKASSTSLTSTQPTKTSGVPSGFNFTAPPVLGKHTEPPVTSSATTTSVAPPAATSTSSTAVFGSLPVTSAGSSGVISFGGTSLSAGKTSFSFGSQQTNSTVPPSAPPPTTAATPLPTSFPTLSFGSLLSSATTPSLPMSAGRSTEEATSSALPEKPGDSEVSASAASLLEEQQSAQLPQAPPQTSDSVKKEPVLAQPAVSNSGTAASSTSLVALSAEATPATTGVPDARTEAVPPASSFSVPGQTAVTAAAISSAGPVAVETSSTPIASSTTSIVAPGPSAEAAAFGTVTSGSSVFAQPPAASSSSAFNQLTNNTATAPSATPVFGQVAASTAPSLFGQQTGSTASTAAATPQVSSSGFSSPAFGTTAPGVFGQTTFGQASVFGQSASSAASVFSFSQPGFSSVPAFGQPASSTPTSTSGSVFGAASSTSSSSSFSFGQSSPNTGGGLFGQSNAPAFGQSPGFGQGGSVFGGTSAATTTAATSGFSFCQASGFGSSNTGSVFGQAASTGGIVFGQQSSSSSGSVFGSGNTGRGGGFFSGLGGKPSQDAANKNPFSSASGGFGSTATSNTSNLFGNSGAKTFGGFASSSFGEQKPTGTFSSGGGSVASQGFGFSSPNKTGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPAFTSPLGSTGGKVFGEGTAAASAGGFGFGSSSNTTSFGTLASQNAPTFGSLSQQTSGFGTQSSGFSGFGSGTGGFSFGSNNSSVQGFGGWRS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
88
Horse
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35658 As Substrate

Site PTM Type Enzyme
G2 Acetylation
S30 Phosphorylation
K36 Ubiquitination
S40 Phosphorylation
S45 Phosphorylation
K72 Ubiquitination
K79 Ubiquitination
K143 Acetylation
S201 Phosphorylation
K205 Ubiquitination
K226 Acetylation
T267 Phosphorylation
S268 Phosphorylation
Y305 Phosphorylation
T416 Phosphorylation
S421 Phosphorylation
S430 Phosphorylation
S433 Phosphorylation
T434 Phosphorylation
T436 Phosphorylation
T437 Phosphorylation
T439 Phosphorylation
S440 Phosphorylation
S441 Phosphorylation
Y502 Phosphorylation
S504 Phosphorylation
S506 Phosphorylation
S508 Phosphorylation
S509 Phosphorylation
T533 Phosphorylation
S537 Phosphorylation
T580 O-Glycosylation
S581 Phosphorylation
S610 Phosphorylation
S621 Phosphorylation
T628 Phosphorylation
S631 Phosphorylation
S636 Phosphorylation
K640 Methylation
S646 Phosphorylation
S648 Phosphorylation
S651 Phosphorylation
S655 Phosphorylation
S656 Phosphorylation
S657 Phosphorylation
S661 Phosphorylation
K665 Methylation
K665 Ubiquitination
S666 Phosphorylation
T670 Phosphorylation
S678 Phosphorylation
S683 Phosphorylation
K691 Acetylation
K691 Ubiquitination
K697 Ubiquitination
K755 Ubiquitination
S760 Phosphorylation
S766 Phosphorylation
K769 Ubiquitination
S792 Phosphorylation
Y794 Phosphorylation
K806 Ubiquitination
S807 Phosphorylation
K822 Ubiquitination
K843 Acetylation
K843 Ubiquitination
K844 Acetylation
T856 Phosphorylation
S911 Phosphorylation
K930 Acetylation
K930 Ubiquitination
S931 Phosphorylation
S940 Phosphorylation
K943 Methylation
K943 Ubiquitination
K953 Ubiquitination
T956 Phosphorylation
T962 Phosphorylation
S966 Phosphorylation
S968 Phosphorylation
S970 Phosphorylation
S974 Phosphorylation
Y977 Phosphorylation
S985 Phosphorylation
S986 Phosphorylation
T987 Phosphorylation
S988 Phosphorylation
S989 Phosphorylation
S996 Phosphorylation
S1002 Phosphorylation
C1003 S-Nitrosylation
T1021 Phosphorylation
S1023 Phosphorylation
S1027 Phosphorylation
K1037 Methylation
S1038 Phosphorylation
S1044 Phosphorylation
S1045 Phosphorylation
T1051 Phosphorylation
T1055 Phosphorylation
S1056 O-Glycosylation
S1056 Phosphorylation
S1058 Phosphorylation
S1081 Phosphorylation
S1083 Phosphorylation
T1112 Phosphorylation
S1114 Phosphorylation
T1134 Phosphorylation
S1136 Phosphorylation
S1141 Phosphorylation
Y1145 Phosphorylation
S1146 O-Glycosylation
T1150 Phosphorylation
T1156 Phosphorylation
S1167 Phosphorylation
S1171 Phosphorylation
T1178 Phosphorylation
S1181 Phosphorylation
S1185 Phosphorylation
S1186 Phosphorylation
T1201 O-Glycosylation
T1203 Phosphorylation
S1211 Phosphorylation
T1229 Phosphorylation
Y1265 Phosphorylation
S1272 Phosphorylation
T1284 Phosphorylation
T1312 Phosphorylation
S1315 Phosphorylation
K1316 Ubiquitination
S1333 Phosphorylation
S1335 Phosphorylation
S1353 Phosphorylation
S1354 O-Glycosylation
S1354 Phosphorylation
T1355 Phosphorylation
S1356 Phosphorylation
T1360 Phosphorylation
S1710 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1)
S1809 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
R1881 Methylation
S1925 Phosphorylation
S1937 Phosphorylation
S1949 Phosphorylation
S1956 Phosphorylation
S1963 Phosphorylation
S1985 Phosphorylation

Research Backgrounds

Function:

Has a critical role in nucleocytoplasmic transport. May serve as a docking site in the receptor-mediated import of substrates across the nuclear pore complex.

PTMs:

Probably glycosylated as it reacts with wheat germ agglutinin (WGA).

Subcellular Location:

Nucleus>Nuclear pore complex.
Note: Cytoplasmic side of the nuclear pore complex.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in thymus, spleen, bone marrow, kidney, brain and testis, but hardly in all other tissues or in whole embryos during development.

Subunit Structure:

Homodimer. Interacts with ZFP36; this interaction increases upon lipopolysaccharide (LPS) stimulation. Interacts with DDX19. Interacts with NUP88. Interacts with XPO1. Interacts with XPO5.

(Microbial infection) Interacts with human herpes virus 1 (HHV-1) protein UL25; this interaction might be essential to the capsid docking onto the host nuclear pore.

Family&Domains:

Contains FG repeats.

The beta-propeller contains long interblade connector loops, and mediates interaction with DDX19B.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

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