NUP133 Antibody - #DF9706

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Product: NUP133 Antibody
Catalog: DF9706
Description: Rabbit polyclonal antibody to NUP133
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 129 kDa; 129kD(Calculated).
Uniprot: Q8WUM0
RRID: AB_2842901

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(90%), Zebrafish(89%), Bovine(90%), Horse(90%), Sheep(90%), Rabbit(90%), Dog(100%), Chicken(90%)
Clonality:
Polyclonal
Specificity:
NUP133 Antibody detects endogenous levels of total NUP133.
RRID:
AB_2842901
Cite Format: Affinity Biosciences Cat# DF9706, RRID:AB_2842901.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

133 kDa nucleoporin; FLJ10814; hNUP 133; hNUP133; MGC21133; NU133_HUMAN; Nuclear pore complex protein Nup133; Nucleoporin 133kDa; Nucleoporin NUP133; NUP 133; NUP133; OTTHUMP00000061095;

Immunogens

Immunogen:
Uniprot:

Q8WUM0(NU133_HUMAN) >>Visit HPA database.

Gene(ID):
NUP133(55746)
Expression:
Q8WUM0 NU133_HUMAN:

Widely expressed in fetal and adult tissues. Expressed in the brain and kidney.

Sequence:
MFPAAPSPRTPGTGSRRGPLAGLGPGSTPRTASRKGLPLGSAVSSPVLFSPVGRRSSLSSRGTPTRMFPHHSITESVNYDVKTFGSSLPVKVMEALTLAEVDDQLTINIDEGGWACLVCKEKLIIWKIALSPITKLSVCKELQLPPSDFHWSADLVALSYSSPSGEAHSTQAVAVMVATREGSIRYWPSLAGEDTYTEAFVDSGGDKTYSFLTAVQGGSFILSSSGSQLIRLIPESSGKIHQHILPQGQGMLSGIGRKVSSLFGILSPSSDLTLSSVLWDRERSSFYSLTSSNISKWELDDSSEKHAYSWDINRALKENITDAIWGSESNYEAIKEGVNIRYLDLKQNCDGLVILAAAWHSADNPCLIYYSLITIEDNGCQMSDAVTVEVTQYNPPFQSEDLILCQLTVPNFSNQTAYLYNESAVYVCSTGTGKFSLPQEKIVFNAQGDSVLGAGACGGVPIIFSRNSGLVSITSRENVSILAEDLEGSLASSVAGPNSESMIFETTTKNETIAQEDKIKLLKAAFLQYCRKDLGHAQMVVDELFSSHSDLDSDSELDRAVTQISVDLMDDYPASDPRWAESVPEEAPGFSNTSLIILHQLEDKMKAHSFLMDFIHQVGLFGRLGSFPVRGTPMATRLLLCEHAEKLSAAIVLKNHHSRLSDLVNTAILIALNKREYEIPSNLTPADVFFREVSQVDTICECLLEHEEQVLRDAPMDSIEWAEVVINVNNILKDMLQAASHYRQNRNSLYRREESLEKEPEYVPWTATSGPGGIRTVIIRQHEIVLKVAYPQADSNLRNIVTEQLVALIDCFLDGYVSQLKSVDKSSNRERYDNLEMEYLQKRSDLLSPLLSLGQYLWAASLAEKYCDFDILVQMCEQTDNQSRLQRYMTQFADQNFSDFLFRWYLEKGKRGKLLSQPISQHGQLANFLQAHEHLSWLHEINSQELEKAHATLLGLANMETRYFAKKKTLLGLSKLAALASDFSEDMLQEKIEEMAEQERFLLHQETLPEQLLAEKQLNLSAMPVLTAPQLIGLYICEENRRANEYDFKKALDLLEYIDEEEDININDLKLEILCKALQRDNWSSSDGKDDPIEVSKDSIFVKILQKLLKDGIQLSEYLPEVKDLLQADQLGSLKSNPYFEFVLKANYEYYVQGQI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Pig
90
Horse
90
Bovine
90
Sheep
90
Chicken
90
Rabbit
90
Zebrafish
89
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WUM0 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S7 Phosphorylation
R9 Methylation
T10 Phosphorylation
T13 Phosphorylation
S15 Phosphorylation
R17 Methylation
S27 Phosphorylation P24941 (CDK2)
T28 Phosphorylation P24941 (CDK2)
T31 Phosphorylation
S33 Phosphorylation
K35 Ubiquitination
S41 Phosphorylation
S44 Phosphorylation
S45 Phosphorylation
S50 Phosphorylation P24941 (CDK2)
S56 Phosphorylation
S57 Phosphorylation
S59 Phosphorylation
S60 Phosphorylation
R61 Methylation
T63 Phosphorylation
T65 Phosphorylation
S72 Phosphorylation
T74 Phosphorylation
S76 Phosphorylation
K82 Sumoylation
K82 Ubiquitination
S131 Phosphorylation
T134 Phosphorylation
K135 Ubiquitination
T197 Phosphorylation
T213 Phosphorylation
S223 Phosphorylation
K239 Ubiquitination
K258 Ubiquitination
S260 Phosphorylation
S261 Phosphorylation
S267 Phosphorylation
K296 Ubiquitination
S303 Phosphorylation
K305 Ubiquitination
K317 Ubiquitination
K335 Ubiquitination
S450 Phosphorylation
S480 Phosphorylation
S489 Phosphorylation
S499 Phosphorylation
T512 Phosphorylation
K518 Ubiquitination
K523 Ubiquitination
S546 Phosphorylation
S547 Phosphorylation
S549 Phosphorylation
S553 Phosphorylation
S582 Phosphorylation
K674 Ubiquitination
S681 Phosphorylation
S755 Phosphorylation
K758 Acetylation
K758 Ubiquitination
Y762 Phosphorylation
K787 Ubiquitination
Y832 Phosphorylation
K842 Ubiquitination
K913 Ubiquitination
T961 Phosphorylation
K967 Ubiquitination
K968 Ubiquitination
T969 Phosphorylation
S981 Phosphorylation
K991 Ubiquitination
S1021 Phosphorylation
T1027 Phosphorylation
Y1046 Phosphorylation
K1049 Ubiquitination
K1076 Ubiquitination
S1084 Phosphorylation
S1085 Phosphorylation
S1086 Phosphorylation
K1089 Ubiquitination
K1097 Ubiquitination
K1107 Ubiquitination
K1110 Ubiquitination
K1123 Ubiquitination
S1133 Phosphorylation
K1135 Ubiquitination
Y1150 Phosphorylation

Research Backgrounds

Function:

Involved in poly(A)+ RNA transport. Involved in nephrogenesis.

Subcellular Location:

Nucleus>Nuclear pore complex. Chromosome>Centromere>Kinetochore.
Note: Located on both the cytoplasmic and nuclear sides of the nuclear pore (PubMed:11564755). During mitosis, localizes to the kinetochores (PubMed:11564755).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed in fetal and adult tissues. Expressed in the brain and kidney.

Subunit Structure:

Forms part of the Nup160 subcomplex in the nuclear pore which is composed of NUP160, NUP133, NUP107 and Nup96. This complex plays a role in RNA export and in tethering Nup98 and NUP153 to the nucleus.

Family&Domains:

Belongs to the nucleoporin Nup133 family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

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