NRXN3 Antibody | Affinity Biosciences

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Product:	NRXN3 Antibody
Catalog:	DF9682
Description:	Rabbit polyclonal antibody to NRXN3
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Horse, Sheep, Chicken
Mol.Wt.:	181 kDa; 181kD(Calculated).
Uniprot:	Q9Y4C0
RRID:	AB_2842878

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Horse(100%), Sheep(100%), Chicken(91%)

Clonality:

Polyclonal

Specificity:

NRXN3 Antibody detects endogenous levels of total NRXN3.

RRID:

AB_2842878
Cite Format: Affinity Biosciences Cat# DF9682, RRID:AB_2842878.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

C14orf60; KIAA0743; Neurexin 3; Neurexin III; Neurexin III-alpha; Neurexin-3; Neurexin-3-alpha [Precursor]; Neurexin-3-alpha; Neurexin-III-beta; NRXN3;

Immunogens

Immunogen:

Uniprot:

Q9Y4C0(NRX3A_HUMAN) >>Visit HPA database.

Gene(ID):

NRXN3(9369)

Expression:

Q9Y4C0 NRX3A_HUMAN:

Expressed in the blood vessel walls (at protein level). Highly expressed in brain, lung, and pancreas; a lower level of expression is detectable in heart, placenta, liver, and kidney, whereas no expression can be observed in skeletal muscle. Isoform 4a is heart-specific.

Sequence:

Q9Y4C0 NRX3A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSSTLHSVFFTLKVSILLGSLLGLCLGLEFMGLPNQWARYLRWDASTRSDLSFQFKTNVSTGLLLYLDDGGVCDFLCLSLVDGRVQLRFSMDCAETAVLSNKQVNDSSWHFLMVSRDRLRTVLMLDGEGQSGELQPQRPYMDVVSDLFLGGVPTDIRPSALTLDGVQAMPGFKGLILDLKYGNSEPRLLGSRGVQMDAEGPCGERPCENGGICFLLDGHPTCDCSTTGYGGKLCSEDVSQDPGLSHLMMSEQAREENVATFRGSEYLCYDLSQNPIQSSSDEITLSFKTWQRNGLILHTGKSADYVNLALKDGAVSLVINLGSGAFEAIVEPVNGKFNDNAWHDVKVTRNLRQVTISVDGILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDIRLELSRLARIADTKMKIYGEVVFKCENVATLDPINFETPEAYISLPKWNTKRMGSISFDFRTTEPNGLILFTHGKPQERKDARSQKNTKVDFFAVELLDGNLYLLLDMGSGTIKVKATQKKANDGEWYHVDIQRDGRSGTISVNSRRTPFTASGESEILDLEGDMYLGGLPENRAGLILPTELWTAMLNYGYVGCIRDLFIDGRSKNIRQLAEMQNAAGVKSSCSRMSAKQCDSYPCKNNAVCKDGWNRFICDCTGTGYWGRTCEREASILSYDGSMYMKIIMPMVMHTEAEDVSFRFMSQRAYGLLVATTSRDSADTLRLELDGGRVKLMVNLDCIRINCNSSKGPETLYAGQKLNDNEWHTVRVVRRGKSLKLTVDDDVAEGTMVGDHTRLEFHNIETGIMTEKRYISVVPSSFIGHLQSLMFNGLLYIDLCKNGDIDYCELKARFGLRNIIADPVTFKTKSSYLSLATLQAYTSMHLFFQFKTTSPDGFILFNSGDGNDFIAVELVKGYIHYVFDLGNGPNVIKGNSDRPLNDNQWHNVVITRDNSNTHSLKVDTKVVTQVINGAKNLDLKGDLYMAGLAQGMYSNLPKLVASRDGFQGCLASVDLNGRLPDLINDALHRSGQIERGCEGPSTTCQEDSCANQGVCMQQWEGFTCDCSMTSYSGNQCNDPGATYIFGKSGGLILYTWPANDRPSTRSDRLAVGFSTTVKDGILVRIDSAPGLGDFLQLHIEQGKIGVVFNIGTVDISIKEERTPVNDGKYHVVRFTRNGGNATLQVDNWPVNEHYPTGRQLTIFNTQAQIAIGGKDKGRLFQGQLSGLYYDGLKVLNMAAENNPNIKINGSVRLVGEVPSILGTTQTTSMPPEMSTTVMETTTTMATTTTRKNRSTASIQPTSDDLVSSAECSSDDEDFVECEPSTTGGELVIPLLVEDPLATPPIATRAPSITLPPTFRPLLTIIETTKDSLSMTSEAGLPCLSDQGSDGCDDDGLVISGYGSGETFDSNLPPTDDEDFYTTFSLVTDKSLSTSIFEGGYKAHAPKWESKDFRPNKVSETSRTTTTSLSPELIRFTASSSSGMVPKLPAGKMNNRDLKPQPDIVLLPLPTAYELDSTKLKSPLITSPMFRNVPTANPTEPGIRRVPGASEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYQVDETRNYISNSAQSNGTLMKEKQQSSKSGHKKQKNKDREYYV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Sheep

100

Chicken

Pig

Bovine

Dog

Xenopus

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y4C0 As Substrate

Q9Y4C0

Site	PTM Type	Source
R88	Methylation	Uniprot
S557	Phosphorylation	Uniprot
S560	Phosphorylation	Uniprot
S620	Phosphorylation	Uniprot
K636	Methylation	Uniprot
S637	Phosphorylation	Uniprot
S638	Phosphorylation	Uniprot
Y719	Phosphorylation	Uniprot
S787	Phosphorylation	Uniprot
T791	Phosphorylation	Uniprot
Y1002	Phosphorylation	Uniprot
S1003	Phosphorylation	Uniprot
S1011	Phosphorylation	Uniprot
T1362	Phosphorylation	Uniprot
T1372	Phosphorylation	Uniprot
S1535	Phosphorylation	Uniprot
S1558	Phosphorylation	Uniprot

Research Backgrounds

Q9Y4C0_NRX3A_HUMAN

Function:

Neuronal cell surface protein that may be involved in cell recognition and cell adhesion. May mediate intracellular signaling.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Tissue Specificity:

Subunit Structure:

The laminin G-like domain 2 binds to NXPH1. Specific isoforms bind to alpha-dystroglycan. The cytoplasmic C-terminal region binds to CASK (By similarity).

Family&Domains:

Belongs to the neurexin family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)

References

1). ZNF582 hypermethylation promotes metastasis of nasopharyngeal carcinoma by regulating the transcription of adhesion molecules Nectin‐3 and NRXN3. Cancer Communications, 2020 (PubMed: 33038291) [IF=16.2]

Application: WB Species: mice Sample: SUEN1 cells

Figure 5. (H) Western blotting of NRXN3 in SUEN1 cells transfected with plasmids encoding shControl or NRXN3-shRNAs.

Application: IHC Species: mice Sample: lung

Figure 7. (K) Immunohistochemical staining for ZNF582, Nectin-3, NRXN3, and IgG in lung metastases. Scale bar: 100 μm.

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NRXN3 Antibody - #DF9682