NDUFC1 Antibody - #DF9667

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Product: NDUFC1 Antibody
Catalog: DF9667
Description: Rabbit polyclonal antibody to NDUFC1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit
Mol.Wt.: 9kDa; 9kD(Calculated).
Uniprot: O43677
RRID: AB_2842863

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Bovine(91%), Horse(91%), Rabbit(91%)
Clonality:
Polyclonal
Specificity:
NDUFC1 Antibody detects endogenous levels of total NDUFC1.
RRID:
AB_2842863
Cite Format: Affinity Biosciences Cat# DF9667, RRID:AB_2842863.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CI-KFYI; Complex I KFYI subunit; Complex I-KFYI; KFYI; mitochondrial; NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 1, 6kDa; NADH dehydrogenase [ubiquinone] 1 subunit C1; NADH dehydrogenase [ubiquinone] 1 subunit C1, mitochondrial; NADH-ubiquinone oxidoreductase KFYI subunit; NDUC1_HUMAN; NDUFC1;

Immunogens

Immunogen:
Uniprot:

O43677(NDUC1_HUMAN) >>Visit HPA database.

Gene(ID):
NDUFC1(4717)
Sequence:
MAPSALLRPLSRLLAPARLPSGPSVRSKFYVREPPNAKPDWLKVGFTLGTTVFLWIYLIKQHNEDILEYKRRNGLE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Horse
91
Bovine
91
Rabbit
91
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43677 As Substrate

Site PTM Type Enzyme
K70 Ubiquitination

Research Backgrounds

Function:

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Subcellular Location:

Mitochondrion inner membrane>Single-pass membrane protein>Matrix side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Complex I is composed of 45 different subunits.

Family&Domains:

Belongs to the complex I NDUFC1 subunit family.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

References

1). Urolithin A protects severe acute pancreatitis-associated acute cardiac injury by regulating mitochondrial fatty acid oxidative metabolism in cardiomyocytes. MedComm, 2023 (PubMed: 38116065) [IF=9.9]

Application: WB    Species: Mouse    Sample: cardiac tissues

FIGURE 2 Effect of Urolithin A on mitochondria‐related genes in SACI mice. (A) Venn diagram of gene expression in sham group vs. SACI group and SACI group vs. UA group. (B) Heatmap analysis of 90 overlapping gene expressions in three groups (n = 4). (C) GO term analysis of 90 overlapping gene expressions in the SACI group vs. UA group. (D) Volcano plots of differential genes in the sham group vs. SACI group and UA group vs. SACI group. (E) Western blotting and quantitative analysis of COX I, COX IV, TOMM7, and NDUFC1 in cardiac tissues of the sham, SACI, and UA groups (n = 3). (F) Representative transmission electron microscopy (TEM) images of cardiac tissues in the sham, SACI, and UA groups (scale bar = 2 μm; 500 nm). (G) Mitochondrial membrane potential changes in isolated purified heart mitochondria in the sham, SACI, and UA groups. (JC‐1 fluorescence: red/green ratio; n = 4). (H) Changes in total ATP concentration in heart tissues in the sham, SACI, and UA groups (n = 6). The results are presented as the mean ± SD. **p < 0.01, ***p < 0.005, ****p < 0.0001 vs. sham group; ##p < 0.01, ###p < 0.005, ####p < 0.0001 vs. SACI group, by one‐way ANOVA tests followed by Tukey tests.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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