Product: NDUFB7 Antibody
Catalog: DF9665
Description: Rabbit polyclonal antibody to NDUFB7
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Xenopus
Mol.Wt.: 16 kDa; 16kD(Calculated).
Uniprot: P17568
RRID: AB_2842861

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(88%), Horse(88%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
NDUFB7 Antibody detects endogenous levels of total NDUFB7.
RRID:
AB_2842861
Cite Format: Affinity Biosciences Cat# DF9665, RRID:AB_2842861.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1110002H15Rik; B18; Cell adhesion protein SQM1; CI B18; CI-B18; Complex I B18; Complex I B18 subunit; Complex I-B18; MGC2480; NADH dehydrogenase (ubiquinone) 1 beta subcomplex 7 18kDa; NADH dehydrogenase (ubiquinone) 1 beta subcomplex 7; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7; NADH ubiquinone oxidoreductase B18 subunit; NADH-ubiquinone oxidoreductase 1 beta subcomplex, 7; NADH-ubiquinone oxidoreductase B18 subunit; NDUB7_HUMAN; Ndufb7;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MGAHLVRRYLGDASVEPDPLQMPTFPPDYGFPERKEREMVATQQEMMDAQLRLQLRDYCAHHLIRLLKCKRDSFPNFLACKQERHDWDYCEHRDYVMRMKEFERERRLLQRKKRREKKAAELAKGQGPGEVDPKVAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
88
Bovine
88
Xenopus
88
Pig
75
Sheep
75
Dog
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P17568 As Substrate

Site PTM Type Enzyme
T24 Phosphorylation
Y29 Phosphorylation
K68 Methylation
S73 Phosphorylation
K81 Ubiquitination
Y89 Phosphorylation
K100 Ubiquitination
K117 Acetylation
K124 Ubiquitination
K134 Ubiquitination

Research Backgrounds

Function:

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Subcellular Location:

Mitochondrion inner membrane>Peripheral membrane protein. Mitochondrion intermembrane space.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Complex I is composed of 45 different subunits.

Family&Domains:

Contains two C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds.

Belongs to the complex I NDUFB7 subunit family.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

Restrictive clause

 

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For Research Use Only.
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