Product: NDUFA7 Antibody
Catalog: DF9662
Description: Rabbit polyclonal antibody to NDUFA7
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 13 kDa; 13kD(Calculated).
Uniprot: O95182
RRID: AB_2842858

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(82%), Bovine(100%), Sheep(91%), Dog(100%), Chicken(82%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
NDUFA7 Antibody detects endogenous levels of total NDUFA7.
RRID:
AB_2842858
Cite Format: Affinity Biosciences Cat# DF9662, RRID:AB_2842858.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

B14.5a; CI-B14.5a; Complex I B14.5a; Complex I-B14.5a; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 7 14.5kDa; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7; NADH ubiquinone oxidoreductase subunit B14.5a; NADH-ubiquinone oxidoreductase subunit B14.5a; NDUA7_HUMAN; NDUFA7;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MASATRLIQRLRNWASGHDLQGKLQLRYQEISKRTQPPPKLPVGPSHKLSNNYYCTRDGRRESVPPSIIMSSQKALVSGKPAESSAVAATEKKAVTPAPPIKRWELSSDQPYL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Sheep
91
Xenopus
90
Zebrafish
82
Chicken
82
Horse
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95182 As Substrate

Site PTM Type Enzyme
R12 Methylation
K23 Ubiquitination
S32 Phosphorylation
Y53 Phosphorylation
S63 Phosphorylation
S67 Phosphorylation
S85 Phosphorylation
T90 Phosphorylation
T96 Phosphorylation

Research Backgrounds

Function:

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Subcellular Location:

Mitochondrion inner membrane>Peripheral membrane protein>Matrix side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Complex I is composed of 45 different subunits.

Family&Domains:

Belongs to the complex I NDUFA7 subunit family.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.