Product: NDUFA5 Antibody
Catalog: DF9660
Description: Rabbit polyclonal antibody to NDUFA5
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Dog
Mol.Wt.: 13 kDa; 13kD(Calculated).
Uniprot: Q16718
RRID: AB_2842856

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(92%), Zebrafish(80%), Bovine(83%), Dog(100%)
Clonality:
Polyclonal
Specificity:
NDUFA5 Antibody detects endogenous levels of total NDUFA5.
RRID:
AB_2842856
Cite Format: Affinity Biosciences Cat# DF9660, RRID:AB_2842856.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

B13; CI 13kB; CI-13kD-B; Complex I subunit B13; Complex I-13kD-B; DKFZp781K1356; FLJ12147; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5 (13kD, B13); NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5; NADH-ubiquinone oxidoreductase 13 kDa-B subunit; NDUA5_HUMAN; NDUFA5; NUFM; Type I dehydrogenase; Ubiquinone reductase; UQOR13;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q16718 NDUA5_HUMAN:

Expressed in all tissues examined with highest levels in heart, skeletal muscle and brain.

Sequence:
MAGVLKKTTGLVGLAVCNTPHERLRILYTKILDVLEEIPKNAAYRKYTEQITNEKLAMVKAEPDVKKLEDQLQGGQLEEVILQAEHELNLARKMREWKLWEPLVEEPPADQWKWPI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Pig
92
Bovine
83
Zebrafish
80
Chicken
55
Horse
0
Sheep
0
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16718 As Substrate

Site PTM Type Enzyme
C17 S-Nitrosylation
Y28 Phosphorylation
K30 Acetylation
K60 Acetylation
K60 Sumoylation

Research Backgrounds

Function:

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Subcellular Location:

Mitochondrion inner membrane>Peripheral membrane protein>Matrix side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all tissues examined with highest levels in heart, skeletal muscle and brain.

Subunit Structure:

Complex I is composed of 45 different subunits.

Family&Domains:

Belongs to the complex I NDUFA5 subunit family.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.