NDUFA1 Antibody | Affinity Biosciences

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Product:	NDUFA1 Antibody
Catalog:	DF9659
Description:	Rabbit polyclonal antibody to NDUFA1
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Chicken, Xenopus
Mol.Wt.:	8 kDa; 8kD(Calculated).
Uniprot:	O15239
RRID:	AB_2842855

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Bovine(90%), Horse(90%), Chicken(88%), Xenopus(80%)

Clonality:

Polyclonal

Specificity:

NDUFA1 Antibody detects endogenous levels of total NDUFA1.

RRID:

AB_2842855
Cite Format: Affinity Biosciences Cat# DF9659, RRID:AB_2842855.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CI MWFE; CI-MWFE; Complex I MWFE; complex I MWFE subunit; Complex I-MWFE; MWFE; MWFE, B. taurus, homolog of; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 1 7.5kDa; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 1; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1; NADH oxidoreductase subunit MWFE; NADH ubiquinone oxidoreductase (complex 1); NADH ubiquinone oxidoreductase MWFE subunit; NADH-ubiquinone oxidoreductase 1 alpha subcomplex, 1; NADH-ubiquinone oxidoreductase MWFE subunit; NDUA1_HUMAN; NDUFA 1; NDUFA1; Type I dehydrogenase; ZNF 183; ZNF183;

Immunogens

Immunogen:

Uniprot:

O15239(NDUA1_HUMAN) >>Visit HPA database.

Gene(ID):

NDUFA1(4694)

Expression:

O15239 NDUA1_HUMAN:

Primarily expressed in heart and skeletal muscle.

Sequence:

O15239 NDUA1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MWFEILPGLSVMGVCLLIPGLATAYIHRFTNGGKEKRVAHFGYHWSLMERDRRISGVDRYYVSKGLENID

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Zebrafish

100

Pig

100

Horse

Bovine

Chicken

Xenopus

Sheep

Dog

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15239 As Substrate

O15239

Site	PTM Type	Source
S55	Phosphorylation	Uniprot
R59	Methylation	Uniprot
Y60	Phosphorylation	Uniprot
Y61	Phosphorylation	Uniprot
S63	Phosphorylation	Uniprot
K64	Ubiquitination	Uniprot

Research Backgrounds

O15239_NDUA1_HUMAN

Function:

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Subcellular Location:

Mitochondrion inner membrane>Single-pass membrane protein>Matrix side.

Tissue Specificity:

Primarily expressed in heart and skeletal muscle.

Subunit Structure:

Complex I is composed of 45 different subunits.

Family&Domains:

Belongs to the complex I NDUFA1 subunit family.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling. (View pathway)

References

1). Proteome characteristics of liver tissue from patients with parenteral nutrition-associated liver disease. Nutrition & Metabolism, 2020 (PubMed: 32518576) [IF=4.5]

Application: WB Species: human Sample: Liver

Fig. 5| Confirmation of differentially expressed proteins (DEPs) by western blotting; a, c: Immunoblotting analysis of DEPs (CYP2B6, DDAH1,NDUFA1, CAPG, and FABP5) in PNALD(H-PN) and control (H-EN).

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NDUFA1 Antibody - #DF9659