Product: NDUFA1 Antibody
Catalog: DF9659
Description: Rabbit polyclonal antibody to NDUFA1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Chicken, Xenopus
Mol.Wt.: 8 kDa; 8kD(Calculated).
Uniprot: O15239
RRID: AB_2842855

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(90%), Horse(90%), Chicken(88%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
NDUFA1 Antibody detects endogenous levels of total NDUFA1.
RRID:
AB_2842855
Cite Format: Affinity Biosciences Cat# DF9659, RRID:AB_2842855.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CI MWFE; CI-MWFE; Complex I MWFE; complex I MWFE subunit; Complex I-MWFE; MWFE; MWFE, B. taurus, homolog of; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 1 7.5kDa; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 1; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1; NADH oxidoreductase subunit MWFE; NADH ubiquinone oxidoreductase (complex 1); NADH ubiquinone oxidoreductase MWFE subunit; NADH-ubiquinone oxidoreductase 1 alpha subcomplex, 1; NADH-ubiquinone oxidoreductase MWFE subunit; NDUA1_HUMAN; NDUFA 1; NDUFA1; Type I dehydrogenase; ZNF 183; ZNF183;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O15239 NDUA1_HUMAN:

Primarily expressed in heart and skeletal muscle.

Sequence:
MWFEILPGLSVMGVCLLIPGLATAYIHRFTNGGKEKRVAHFGYHWSLMERDRRISGVDRYYVSKGLENID

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Zebrafish
100
Pig
100
Horse
90
Bovine
90
Chicken
88
Xenopus
80
Sheep
0
Dog
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15239 As Substrate

Site PTM Type Enzyme
S55 Phosphorylation
R59 Methylation
Y60 Phosphorylation
Y61 Phosphorylation
S63 Phosphorylation
K64 Ubiquitination

Research Backgrounds

Function:

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Subcellular Location:

Mitochondrion inner membrane>Single-pass membrane protein>Matrix side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Primarily expressed in heart and skeletal muscle.

Subunit Structure:

Complex I is composed of 45 different subunits.

Family&Domains:

Belongs to the complex I NDUFA1 subunit family.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

References

1). Proteome characteristics of liver tissue from patients with parenteral nutrition-associated liver disease. Nutrition & Metabolism, 2020 (PubMed: 32518576) [IF=4.5]

Application: WB    Species: human    Sample: Liver

Fig. 5| Confirmation of differentially expressed proteins (DEPs) by western blotting; a, c: Immunoblotting analysis of DEPs (CYP2B6, DDAH1,NDUFA1, CAPG, and FABP5) in PNALD(H-PN) and control (H-EN).

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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