MYH3 Antibody - #DF9647

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Product: MYH3 Antibody
Catalog: DF9647
Description: Rabbit polyclonal antibody to MYH3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit
Mol.Wt.: 224kDa; 224kD(Calculated).
Uniprot: P11055
RRID: AB_2842843

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(91%)
Clonality:
Polyclonal
Specificity:
MYH3 Antibody detects endogenous levels of total MYH3.
RRID:
AB_2842843
Cite Format: Affinity Biosciences Cat# DF9647, RRID:AB_2842843.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

adult 1; MYH1; MYH1_HUMAN; MYH2; MYH3; MYH4; MYH6; MyHC-2x; MyHC-IIx/d; MYL1; MYL2; MYL3; MYL5; MYL6; Myosin light chain 2 regulatory cardiac slow; Myosin 1; Myosin heavy chain 1; Myosin heavy chain 1 skeletal muscle adult; Myosin heavy chain 2 skeletal muscle adult; Myosin heavy chain 2x; Myosin heavy chain 3 skeletal muscle adult; Myosin heavy chain 4 skeletal muscle adult; Myosin heavy chain 6 skeletal muscle adult; Myosin heavy chain; Myosin heavy chain IIx/d; Myosin heavy chain skeletal muscle adult 1; Myosin II; Myosin light chain 1 alkali skeletal fast; Myosin light chain 3 alkali ventricular skeletal slow; Myosin light chain 4 alkali atrial embryonic; Myosin light chain 5 regulatory; Myosin light chain 6 alkali smooth muscle and non muscle; Myosin-1; Myosin1; skeletal muscle;

Immunogens

Immunogen:
Uniprot:

P11055(MYH3_HUMAN) >>Visit HPA database.

Gene(ID):
MYH3(4621)
Expression:
P11055 MYH3_HUMAN:

Expressed in fetal bone, thymus, placenta, heart, brain, and liver.

Sequence:
MSSDTEMEVFGIAAPFLRKSEKERIEAQNQPFDAKTYCFVVDSKEEYAKGKIKSSQDGKVTVETEDNRTLVVKPEDVYAMNPPKFDRIEDMAMLTHLNEPAVLYNLKDRYTSWMIYTYSGLFCVTVNPYKWLPVYNPEVVEGYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAATGDLAKKKDSKMKGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQILSNKKPELIELLLITTNPYDYPFISQGEILVASIDDAEELLATDSAIDILGFTPEEKSGLYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKTAYLMGLNSSDLLKALCFPRVKVGNEYVTKGQTVDQVHHAVNALSKSVYEKLFLWMVTRINQQLDTKLPRQHFIGVLDIAGFEIFEYNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSNNFQKPKVVKGRAEAHFSLIHYAGTVDYSVSGWLEKNKDPLNETVVGLYQKSSNRLLAHLYATFATADADSGKKKVAKKKGSSFQTVSALFRENLNKLMSNLRTTHPHFVRCIIPNETKTPGAMEHSLVLHQLRCNGVLEGIRICRKGFPNRILYGDFKQRYRVLNASAIPEGQFIDSKKACEKLLASIDIDHTQYKFGHTKVFFKAGLLGTLEEMRDDRLAKLITRTQAVCRGFLMRVEFQKMVQRRESIFCIQYNIRSFMNVKHWPWMKLFFKIKPLLKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKLVTLVQEKNDLQLQVQAESENLLDAEERCDQLIKAKFQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEELSGLDETIAKLTREKKALQEAHQQALDDLQAEEDKVNSLNKTKSKLEQQVEDLESSLEQEKKLRVDLERNKRKLEGDLKLAQESILDLENDKQQLDERLKKKDFEYCQLQSKVEDEQTLGLQFQKKIKELQARIEELEEEIEAERATRAKTEKQRSDYARELEELSERLEEAGGVTSTQIELNKKREAEFLKLRRDLEEATLQHEAMVAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLEIDDLSSSMESVSKSKANLEKICRTLEDQLSEARGKNEEIQRSLSELTTQKSRLQTEAGELSRQLEEKESIVSQLSRSKQAFTQQTEELKRQLEEENKAKNALAHALQSSRHDCDLLREQYEEEQEGKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDSEEQVEAVNAKCASLEKTKQRLQGEVEDLMVDVERANSLAAALDKKQRNFDKVLAEWKTKCEESQAELEASLKESRSLSTELFKLKNAYEEALDQLETVKRENKNLEQEIADLTEQIAENGKTIHELEKSRKQIELEKADIQLALEEAEAALEHEEAKILRIQLELTQVKSEIDRKIAEKDEEIEQLKRNYQRTVETMQSALDAEVRSRNEAIRLKKKMEGDLNEIEIQLSHANRQAAETLKHLRSVQGQLKDTQLHLDDALRGQEDLKEQLAIVERRANLLQAEVEELRATLEQTERARKLAEQELLDSNERVQLLHTQNTSLIHTKKKLETDLMQLQSEVEDASRDARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLETRIRELEFELEGEQKKNTESVKGLRKYERRVKELTYQSEEDRKNVLRLQDLVDKLQVKVKSYKRQAEEADEQANAHLTKFRKAQHELEEAEERADIAESQVNKLRAKTRDFTSSRMVVHESEE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
91
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P11055 As Substrate

Site PTM Type Enzyme
K44 Acetylation
Y78 Phosphorylation
Y110 Phosphorylation
Y163 Phosphorylation
T168 Phosphorylation
K247 Ubiquitination
T256 Phosphorylation
S261 Phosphorylation
Y267 Phosphorylation
K271 Ubiquitination
T354 Phosphorylation
Y360 Phosphorylation
T379 Phosphorylation
Y411 Phosphorylation
T413 Phosphorylation
K414 Acetylation
K503 Acetylation
Y554 Phosphorylation
Y610 Phosphorylation
Y716 Phosphorylation
Y723 Phosphorylation
S729 Phosphorylation
S739 Phosphorylation
Y817 Phosphorylation
K832 Acetylation
K842 Acetylation
T852 Phosphorylation
T860 Phosphorylation
K872 Acetylation
S949 Phosphorylation
K952 Acetylation
K953 Acetylation
T961 Phosphorylation
S984 Phosphorylation
S1066 Phosphorylation
T1129 Phosphorylation
T1133 Phosphorylation
S1138 Phosphorylation
Y1140 Phosphorylation
S1200 Phosphorylation
S1336 Phosphorylation
S1367 Phosphorylation
T1374 Phosphorylation
Y1376 Phosphorylation
T1378 Phosphorylation
T1384 Phosphorylation
K1391 Acetylation
K1392 Acetylation
K1393 Acetylation
T1418 Phosphorylation
S1477 Phosphorylation
S1479 Phosphorylation
T1480 Phosphorylation
Y1489 Phosphorylation
T1733 Phosphorylation
S1740 Phosphorylation
T1761 Phosphorylation
K1771 Acetylation
K1832 Acetylation
K1877 Acetylation
K1880 Acetylation
S1916 Phosphorylation

Research Backgrounds

Function:

Muscle contraction.

Subcellular Location:

Cytoplasm>Myofibril.
Note: Thick filaments of the myofibrils.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in fetal bone, thymus, placenta, heart, brain, and liver.

Subunit Structure:

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

Family&Domains:

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.

Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

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