MLL2 Antibody - #DF9646

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Product: MLL2 Antibody
Catalog: DF9646
Description: Rabbit polyclonal antibody to MLL2
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 593kDa; 593kD(Calculated).
Uniprot: O14686
RRID: AB_2842842

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(83%), Dog(100%)
Clonality:
Polyclonal
Specificity:
MLL2 Antibody detects endogenous levels of total MLL2.
RRID:
AB_2842842
Cite Format: Affinity Biosciences Cat# DF9646, RRID:AB_2842842.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AAD10; ALL1 related gene; ALL1-related protein; ALR; CAGL114; Histone-lysine N-methyltransferase MLL2; KABUK1; Kabuki make up syndrome; Kabuki mental retardation syndrome; KMS; KMT2B; KMT2D; Lysine N methyltransferase 2D; Lysine N-methyltransferase 2B; MLL2; MLL2_HUMAN; MLL4; Myeloid/lymphoid or mixed lineage leukemia 2; Myeloid/lymphoid or mixed-lineage leukemia protein 2; TNRC21; Trinucleotide repeat containing 21;

Immunogens

Immunogen:
Uniprot:

O14686(KMT2D_HUMAN) >>Visit HPA database.

Gene(ID):
KMT2D(8085)
Expression:
O14686 KMT2D_HUMAN:

Expressed in most adult tissues, including a variety of hematoipoietic cells, with the exception of the liver.

Sequence:
MDSQKLAGEDKDSEPAADGPAASEDPSATESDLPNPHVGEVSVLSSGSPRLQETPQDCSGGPVRRCALCNCGEPSLHGQRELRRFELPFDWPRCPVVSPGGSPGPNEAVLPSEDLSQIGFPEGLTPAHLGEPGGSCWAHHWCAAWSAGVWGQEGPELCGVDKAIFSGISQRCSHCTRLGASIPCRSPGCPRLYHFPCATASGSFLSMKTLQLLCPEHSEGAAYLEEARCAVCEGPGELCDLFFCTSCGHHYHGACLDTALTARKRAGWQCPECKVCQACRKPGNDSKMLVCETCDKGYHTFCLKPPMEELPAHSWKCKACRVCRACGAGSAELNPNSEWFENYSLCHRCHKAQGGQTIRSVAEQHTPVCSRFSPPEPGDTPTDEPDALYVACQGQPKGGHVTSMQPKEPGPLQCEAKPLGKAGVQLEPQLEAPLNEEMPLLPPPEESPLSPPPEESPTSPPPEASRLSPPPEELPASPLPEALHLSRPLEESPLSPPPEESPLSPPPESSPFSPLEESPLSPPEESPPSPALETPLSPPPEASPLSPPFEESPLSPPPEELPTSPPPEASRLSPPPEESPMSPPPEESPMSPPPEASRLFPPFEESPLSPPPEESPLSPPPEASRLSPPPEDSPMSPPPEESPMSPPPEVSRLSPLPVVSRLSPPPEESPLSPPPEESPTSPPPEASRLSPPPEDSPTSPPPEDSPASPPPEDSLMSLPLEESPLLPLPEEPQLCPRSEGPHLSPRPEEPHLSPRPEEPHLSPQAEEPHLSPQPEEPCLCAVPEEPHLSPQAEGPHLSPQPEELHLSPQTEEPHLSPVPEEPCLSPQPEESHLSPQSEEPCLSPRPEESHLSPELEKPPLSPRPEKPPEEPGQCPAPEELPLFPPPGEPSLSPLLGEPALSEPGEPPLSPLPEELPLSPSGEPSLSPQLMPPDPLPPPLSPIITAAAPPALSPLGELEYPFGAKGDSDPESPLAAPILETPISPPPEANCTDPEPVPPMILPPSPGSPVGPASPILMEPLPPQCSPLLQHSLVPQNSPPSQCSPPALPLSVPSPLSPIGKVVGVSDEAELHEMETEKVSEPECPALEPSATSPLPSPMGDLSCPAPSPAPALDDFSGLGEDTAPLDGIDAPGSQPEPGQTPGSLASELKGSPVLLDPEELAPVTPMEVYPECKQTAGQGSPCEEQEEPRAPVAPTPPTLIKSDIVNEISNLSQGDASASFPGSEPLLGSPDPEGGGSLSMELGVSTDVSPARDEGSLRLCTDSLPETDDSLLCDAGTAISGGKAEGEKGRRRSSPARSRIKQGRSSSFPGRRRPRGGAHGGRGRGRARLKSTASSIETLVVADIDSSPSKEEEEEDDDTMQNTVVLFSNTDKFVLMQDMCVVCGSFGRGAEGHLLACSQCSQCYHPYCVNSKITKVMLLKGWRCVECIVCEVCGQASDPSRLLLCDDCDISYHTYCLDPPLLTVPKGGWKCKWCVSCMQCGAASPGFHCEWQNSYTHCGPCASLVTCPICHAPYVEEDLLIQCRHCERWMHAGCESLFTEDDVEQAADEGFDCVSCQPYVVKPVAPVAPPELVPMKVKEPEPQYFRFEGVWLTETGMALLRNLTMSPLHKRRQRRGRLGLPGEAGLEGSEPSDALGPDDKKDGDLDTDELLKGEGGVEHMECEIKLEGPVSPDVEPGKEETEESKKRKRKPYRPGIGGFMVRQRKSHTRTKKGPAAQAEVLSGDGQPDEVIPADLPAEGAVEQSLAEGDEKKKQQRRGRKKSKLEDMFPAYLQEAFFGKELLDLSRKALFAVGVGRPSFGLGTPKAKGDGGSERKELPTSQKGDDGPDIADEESRGLEGKADTPGPEDGGVKASPVPSDPEKPGTPGEGMLSSDLDRISTEELPKMESKDLQQLFKDVLGSEREQHLGCGTPGLEGSRTPLQRPFLQGGLPLGNLPSSSPMDSYPGLCQSPFLDSRERGGFFSPEPGEPDSPWTGSGGTTPSTPTTPTTEGEGDGLSYNQRSLQRWEKDEELGQLSTISPVLYANINFPNLKQDYPDWSSRCKQIMKLWRKVPAADKAPYLQKAKDNRAAHRINKVQKQAESQINKQTKVGDIARKTDRPALHLRIPPQPGALGSPPPAAAPTIFIGSPTTPAGLSTSADGFLKPPAGSVPGPDSPGELFLKLPPQVPAQVPSQDPFGLAPAYPLEPRFPTAPPTYPPYPSPTGAPAQPPMLGASSRPGAGQPGEFHTTPPGTPRHQPSTPDPFLKPRCPSLDNLAVPESPGVGGGKASEPLLSPPPFGESRKALEVKKEELGASSPSYGPPNLGFVDSPSSGTHLGGLELKTPDVFKAPLTPRASQVEPQSPGLGLRPQEPPPAQALAPSPPSHPDIFRPGSYTDPYAQPPLTPRPQPPPPESCCALPPRSLPSDPFSRVPASPQSQSSSQSPLTPRPLSAEAFCPSPVTPRFQSPDPYSRPPSRPQSRDPFAPLHKPPRPQPPEVAFKAGSLAHTSLGAGGFPAALPAGPAGELHAKVPSGQPPNFVRSPGTGAFVGTPSPMRFTFPQAVGEPSLKPPVPQPGLPPPHGINSHFGPGPTLGKPQSTNYTVATGNFHPSGSPLGPSSGSTGESYGLSPLRPPSVLPPPAPDGSLPYLSHGASQRSGITSPVEKREDPGTGMGSSLATAELPGTQDPGMSGLSQTELEKQRQRQRLRELLIRQQIQRNTLRQEKETAAAAAGAVGPPGSWGAEPSSPAFEQLSRGQTPFAGTQDKSSLVGLPPSKLSGPILGPGSFPSDDRLSRPPPPATPSSMDVNSRQLVGGSQAFYQRAPYPGSLPLQQQQQQLWQQQQATAATSMRFAMSARFPSTPGPELGRQALGSPLAGISTRLPGPGEPVPGPAGPAQFIELRHNVQKGLGPGGTPFPGQGPPQRPRFYPVSEDPHRLAPEGLRGLAVSGLPPQKPSAPPAPELNNSLHPTPHTKGPTLPTGLELVNRPPSSTELGRPNPLALEAGKLPCEDPELDDDFDAHKALEDDEELAHLGLGVDVAKGDDELGTLENLETNDPHLDDLLNGDEFDLLAYTDPELDTGDKKDIFNEHLRLVESANEKAEREALLRGVEPGPLGPEERPPPAADASEPRLASVLPEVKPKVEEGGRHPSPCQFTIATPKVEPAPAANSLGLGLKPGQSMMGSRDTRMGTGPFSSSGHTAEKASFGATGGPPAHLLTPSPLSGPGGSSLLEKFELESGALTLPGGPAASGDELDKMESSLVASELPLLIEDLLEHEKKELQKKQQLSAQLQPAQQQQQQQQQHSLLSAPGPAQAMSLPHEGSSPSLAGSQQQLSLGLAGARQPGLPQPLMPTQPPAHALQQRLAPSMAMVSNQGHMLSGQHGGQAGLVPQQSSQPVLSQKPMGTMPPSMCMKPQQLAMQQQLANSFFPDTDLDKFAAEDIIDPIAKAKMVALKGIKKVMAQGSIGVAPGMNRQQVSLLAQRLSGGPSSDLQNHVAAGSGQERSAGDPSQPRPNPPTFAQGVINEADQRQYEEWLFHTQQLLQMQLKVLEEQIGVHRKSRKALCAKQRTAKKAGREFPEADAEKLKLVTEQQSKIQKQLDQVRKQQKEHTNLMAEYRNKQQQQQQQQQQQQQQHSAVLALSPSQSPRLLTKLPGQLLPGHGLQPPQGPPGGQAGGLRLTPGGMALPGQPGGPFLNTALAQQQQQQHSGGAGSLAGPSGGFFPGNLALRSLGPDSRLLQERQLQLQQQRMQLAQKLQQQQQQQQQQQHLLGQVAIQQQQQQGPGVQTNQALGPKPQGLMPPSSHQGLLVQQLSPQPPQGPQGMLGPAQVAVLQQQHPGALGPQGPHRQVLMTQSRVLSSPQLAQQGQGLMGHRLVTAQQQQQQQQHQQQGSMAGLSHLQQSLMSHSGQPKLSAQPMGSLQQLQQQQQLQQQQQLQQQQQQQLQQQQQLQQQQLQQQQQQQQLQQQQQQQLQQQQQQLQQQQQQQQQQFQQQQQQQQMGLLNQSRTLLSPQQQQQQQVALGPGMPAKPLQHFSSPGALGPTLLLTGKEQNTVDPAVSSEATEGPSTHQGGPLAIGTTPESMATEPGEVKPSLSGDSQLLLVQPQPQPQPSSLQLQPPLRLPGQQQQQVSLLHTAGGGSHGQLGSGSSSEASSVPHLLAQPSVSLGDQPGSMTQNLLGPQQPMLERPMQNNTGPQPPKPGPVLQSGQGLPGVGIMPTVGQLRAQLQGVLAKNPQLRHLSPQQQQQLQALLMQRQLQQSQAVRQTPPYQEPGTQTSPLQGLLGCQPQLGGFPGPQTGPLQELGAGPRPQGPPRLPAPPGALSTGPVLGPVHPTPPPSSPQEPKRPSQLPSPSSQLPTEAQLPPTHPGTPKPQGPTLEPPPGRVSPAAAQLADTLFSKGLGPWDPPDNLAETQKPEQSSLVPGHLDQVNGQVVPEASQLSIKQEPREEPCALGAQSVKREANGEPIGAPGTSNHLLLAGPRSEAGHLLLQKLLRAKNVQLSTGRGSEGLRAEINGHIDSKLAGLEQKLQGTPSNKEDAAARKPLTPKPKRVQKASDRLVSSRKKLRKEDGVRASEALLKQLKQELSLLPLTEPAITANFSLFAPFGSGCPVNGQSQLRGAFGSGALPTGPDYYSQLLTKNNLSNPPTPPSSLPPTPPPSVQQKMVNGVTPSEELGEHPKDAASARDSERALRDTSEVKSLDLLAALPTPPHNQTEDVRMESDEDSDSPDSIVPASSPESILGEEAPRFPHLGSGRWEQEDRALSPVIPLIPRASIPVFPDTKPYGALGLEVPGKLPVTTWEKGKGSEVSVMLTVSAAAAKNLNGVMVAVAELLSMKIPNSYEVLFPESPARAGTEPKKGEAEGPGGKEKGLEGKSPDTGPDWLKQFDAVLPGYTLKSQLDILSLLKQESPAPEPPTQHSYTYNVSNLDVRQLSAPPPEEPSPPPSPLAPSPASPPTEPLVELPTEPLAEPPVPSPLPLASSPESARPKPRARPPEEGEDSRPPRLKKWKGVRWKRLRLLLTIQKGSGRQEDEREVAEFMEQLGTALRPDKVPRDMRRCCFCHEEGDGATDGPARLLNLDLDLWVHLNCALWSTEVYETQGGALMNVEVALHRGLLTKCSLCQRTGATSSCNRMRCPNVYHFACAIRAKCMFFKDKTMLCPMHKIKGPCEQELSSFAVFRRVYIERDEVKQIASIIQRGERLHMFRVGGLVFHAIGQLLPHQMADFHSATALYPVGYEATRIYWSLRTNNRRCCYRCSIGENNGRPEFVIKVIEQGLEDLVFTDASPQAVWNRIIEPVAAMRKEADMLRLFPEYLKGEELFGLTVHAVLRIAESLPGVESCQNYLFRYGRHPLMELPLMINPTGCARSEPKILTHYKRPHTLNSTSMSKAYQSTFTGETNTPYSKQFVHSKSSQYRRLRTEWKNNVYLARSRIQGLGLYAAKDLEKHTMVIEYIGTIIRNEVANRREKIYEEQNRGIYMFRINNEHVIDATLTGGPARYINHSCAPNCVAEVVTFDKEDKIIIISSRRIPKGEELTYDYQFDFEDDQHKIPCHCGAWNCRKWMN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
83
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O14686 As Substrate

Site PTM Type Enzyme
S27 Phosphorylation
T29 Phosphorylation
S31 Phosphorylation
S48 Phosphorylation
T54 Phosphorylation
S102 Phosphorylation
Y223 Phosphorylation
Y298 Phosphorylation
S314 Phosphorylation
T366 Phosphorylation
S373 Phosphorylation
T380 Phosphorylation
Y389 Phosphorylation
S468 Phosphorylation
S477 Phosphorylation
S609 Phosphorylation
S618 Phosphorylation
S654 Phosphorylation
S952 Phosphorylation
S1037 Phosphorylation
S1065 Phosphorylation
S1151 Phosphorylation
T1164 Phosphorylation
S1180 Phosphorylation
T1195 Phosphorylation
T1198 Phosphorylation
S1249 Phosphorylation
S1263 Phosphorylation
T1267 Phosphorylation
S1270 Phosphorylation
S1293 Phosphorylation
S1294 Phosphorylation
S1298 Phosphorylation
S1307 Phosphorylation
S1331 Phosphorylation
T1332 Phosphorylation
S1334 Phosphorylation
S1335 Phosphorylation
S1606 Phosphorylation
K1610 Ubiquitination
T1647 Phosphorylation
S1671 Phosphorylation
K1690 Acetylation
Y1692 Phosphorylation
S1722 Phosphorylation
S1762 Phosphorylation
S1798 Phosphorylation
T1803 Phosphorylation
K1815 Acetylation
T1819 Phosphorylation
S1820 Phosphorylation
K1822 Acetylation
S1834 Phosphorylation
T1843 Phosphorylation
S1854 Phosphorylation
S1858 Phosphorylation
T1865 Phosphorylation
S1872 Phosphorylation
K1896 Ubiquitination
T1911 Phosphorylation
S1950 Phosphorylation
T2097 Phosphorylation
S2128 Phosphorylation
T2131 Phosphorylation
S2155 Phosphorylation
S2201 Phosphorylation
T2228 Phosphorylation
T2229 Phosphorylation
T2233 Phosphorylation
S2239 Phosphorylation
T2240 Phosphorylation
K2246 Acetylation
S2251 Phosphorylation
S2260 Phosphorylation
S2274 Phosphorylation
S2295 Phosphorylation
S2296 Phosphorylation
S2298 Phosphorylation
Y2299 Phosphorylation
S2309 Phosphorylation
S2311 Phosphorylation
T2314 Phosphorylation
T2323 Phosphorylation
T2332 Phosphorylation
S2342 Phosphorylation
T2426 Phosphorylation
S2451 Phosphorylation
K2468 Acetylation
R2471 Methylation
S2512 Phosphorylation
S2636 Phosphorylation
S2640 Phosphorylation
S2725 Phosphorylation
S2726 Phosphorylation
S2733 Phosphorylation
T2742 Phosphorylation
K2755 Acetylation
R2774 Methylation
R2860 Methylation
R2881 Methylation
K2886 Methylation
R2903 Methylation
R2905 Methylation
R2915 Methylation
R2922 Methylation
T2949 Phosphorylation
S2969 Phosphorylation
K3079 Acetylation
K3119 Acetylation
K3121 Acetylation
S3130 Phosphorylation
T3138 Phosphorylation
K3155 Acetylation
T3179 Phosphorylation
K3182 Acetylation
T3197 Phosphorylation
S3199 Phosphorylation
S3202 Phosphorylation
S3229 Phosphorylation
K3433 Acetylation
S3443 Phosphorylation
S3463 Phosphorylation
S3620 Phosphorylation
S3624 Phosphorylation
R3626 Methylation
R3719 Methylation
R3727 Methylation
S3832 Phosphorylation
S3836 Phosphorylation
S3837 Phosphorylation
R3851 Methylation
S3986 Phosphorylation
K4004 Acetylation
S4011 Phosphorylation
R4212 Methylation
S4215 Phosphorylation
S4359 Phosphorylation
S4430 Phosphorylation
S4456 Phosphorylation
K4465 Acetylation
K4465 Ubiquitination
K4470 Ubiquitination
S4475 Phosphorylation
K4501 Ubiquitination
T4505 Phosphorylation
K4509 Acetylation
K4516 Acetylation
T4519 Phosphorylation
S4534 Phosphorylation
K4553 Ubiquitination
S4617 Phosphorylation
T4621 Phosphorylation
S4624 Phosphorylation
S4625 Phosphorylation
T4629 Phosphorylation
S4633 Phosphorylation
T4643 Phosphorylation
S4661 Phosphorylation
T4682 Phosphorylation
T4688 Phosphorylation
S4727 Phosphorylation
S4738 Phosphorylation
K4756 Acetylation
K4756 Sumoylation
K4776 Acetylation
S4780 Phosphorylation
S4783 Phosphorylation
T4787 Phosphorylation
S4789 Phosphorylation
K4794 Acetylation
S4822 Phosphorylation
S4849 Phosphorylation
T4852 Phosphorylation
K4870 Ubiquitination
S4871 Phosphorylation
S4883 Phosphorylation
S4955 Phosphorylation
R4966 Methylation
S4974 Phosphorylation
R4975 Methylation
R4978 Methylation
K5024 Methylation
K5276 Acetylation
T5297 Phosphorylation
S5341 Phosphorylation
T5347 Phosphorylation
Y5349 Phosphorylation
S5361 Phosphorylation
T5374 Phosphorylation P24941 (CDK2)
K5378 Ubiquitination
Y5412 Phosphorylation
S5498 Phosphorylation
S5499 Phosphorylation

Research Backgrounds

Function:

Histone methyltransferase. Methylates 'Lys-4' of histone H3 (H3K4me). H3K4me represents a specific tag for epigenetic transcriptional activation. Acts as a coactivator for estrogen receptor by being recruited by ESR1, thereby activating transcription.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in most adult tissues, including a variety of hematoipoietic cells, with the exception of the liver.

Subunit Structure:

Component of the MLL2/3 complex (also named ASCOM complex), at least composed of KMT2D/MLL2 or KMT2C/MLL3, ASH2L, RBBP5, WDR5, NCOA6, DPY30, KDM6A, PAXIP1/PTIP, PAGR1 and alpha- and beta-tubulin. Interacts with ESR1; interaction is direct. Interacts (via WIN motif) with WDR5.

Family&Domains:

LXXLL motifs 5 and 6 are essential for the association with ESR1 nuclear receptor.

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. TRX/MLL subfamily.

Research Fields

· Metabolism > Amino acid metabolism > Lysine degradation.

References

1). KMT2D Regulates the NCOA6/THRB Signal Axis through Epigenetic Modification to Promote the Migration and Invasion of Papillary Thyroid Cancer. Frontiers in Bioscience - Elite, 2023 (PubMed: 36722273)

Application: WB    Species: Human    Sample: Nthy-ori3-1, TPC, IHH-4 and BCPAP cells

Fig. 1. The mRNA and protein expressions of KMT2D in Nthy-ori3-1, TPC, IHH-4 and BCPAP cells. (a) qRT-PCR analysis. (b) Western blotting analysis. A: Nthy-ori3-1 cell; B: TPC cell; C: IHH-4 cell; D: BCPAP cell.

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