Product: MRPL27 Antibody
Catalog: DF9627
Description: Rabbit polyclonal antibody to MRPL27
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Sheep
Mol.Wt.: 16 kDa; 16kD(Calculated).
Uniprot: Q9P0M9
RRID: AB_2842823

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(82%), Horse(82%), Sheep(82%)
Clonality:
Polyclonal
Specificity:
MRPL27 Antibody detects endogenous levels of total MRPL27.
RRID:
AB_2842823
Cite Format: Affinity Biosciences Cat# DF9627, RRID:AB_2842823.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

39S ribosomal protein L27; HSPC250; L27mt; mitochondrial; MRP-L27; mrpl27; RM27_HUMAN;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MASVVLALRTRTAVTSLLSPTPATALAVRYASKKSGGSSKNLGGKSSGRRQGIKKMEGHYVHAGNIIATQRHFRWHPGAHVGVGKNKCLYALEEGIVRYTKEVYVPHPRNTEAVDLITRLPKGAVLYKTFVHVVPAKPEGTFKLVAML

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
82
Horse
82
Sheep
82
Bovine
73
Rabbit
73
Dog
64
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9P0M9 As Substrate

Site PTM Type Enzyme
T15 Phosphorylation
S16 Phosphorylation
S32 Phosphorylation
K45 Ubiquitination
S46 Phosphorylation
S47 Phosphorylation
K85 Ubiquitination
K87 Ubiquitination
K101 Ubiquitination
K122 Ubiquitination
K137 Ubiquitination

Research Backgrounds

Subcellular Location:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the mitochondrial large ribosomal subunit (mt-LSU). Mature mammalian 55S mitochondrial ribosomes consist of a small (28S) and a large (39S) subunit. The 28S small subunit contains a 12S ribosomal RNA (12S mt-rRNA) and 30 different proteins. The 39S large subunit contains a 16S rRNA (16S mt-rRNA), a copy of mitochondrial valine transfer RNA (mt-tRNA(Val)), which plays an integral structural role, and 52 different proteins.

Family&Domains:

Belongs to the bacterial ribosomal protein bL27 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

Restrictive clause

 

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For Research Use Only.
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