LRP4 Antibody - #DF9610

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Product: LRP4 Antibody
Catalog: DF9610
Description: Rabbit polyclonal antibody to LRP4
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 212kDa; 212kD(Calculated).
Uniprot: O75096
RRID: AB_2842806

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(89%), Dog(100%), Chicken(89%), Xenopus(89%)
Clonality:
Polyclonal
Specificity:
LRP4 Antibody detects endogenous levels of total LRP4.
RRID:
AB_2842806
Cite Format: Affinity Biosciences Cat# DF9610, RRID:AB_2842806.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

KIAA0816; LDLR dan; Low density lipoprotein receptor related protein 4; Low-density lipoprotein receptor-related protein 4; LRP-4; LRP10; Lrp4; LRP4_HUMAN; MEGF7; Multiple epidermal growth factor like domains 7; Multiple epidermal growth factor-like domains 7;

Immunogens

Immunogen:
Uniprot:

O75096(LRP4_HUMAN) >>Visit HPA database.

Gene(ID):
LRP4(4038)
Expression:
O75096 LRP4_HUMAN:

Expressed in bone; present in osteoblasts and osteocytes. No expression is observed in osteoclast. Expressed in several regions of the brain.

Sequence:
MRRQWGALLLGALLCAHGLASSPECACGRSHFTCAVSALGECTCIPAQWQCDGDNDCGDHSDEDGCILPTCSPLDFHCDNGKCIRRSWVCDGDNDCEDDSDEQDCPPRECEEDEFPCQNGYCIRSLWHCDGDNDCGDNSDEQCDMRKCSDKEFRCSDGSCIAEHWYCDGDTDCKDGSDEENCPSAVPAPPCNLEEFQCAYGRCILDIYHCDGDDDCGDWSDESDCSSHQPCRSGEFMCDSGLCINAGWRCDGDADCDDQSDERNCTTSMCTAEQFRCHSGRCVRLSWRCDGEDDCADNSDEENCENTGSPQCALDQFLCWNGRCIGQRKLCNGVNDCGDNSDESPQQNCRPRTGEENCNVNNGGCAQKCQMVRGAVQCTCHTGYRLTEDGHTCQDVNECAEEGYCSQGCTNSEGAFQCWCETGYELRPDRRSCKALGPEPVLLFANRIDIRQVLPHRSEYTLLLNNLENAIALDFHHRRELVFWSDVTLDRILRANLNGSNVEEVVSTGLESPGGLAVDWVHDKLYWTDSGTSRIEVANLDGAHRKVLLWQNLEKPRAIALHPMEGTIYWTDWGNTPRIEASSMDGSGRRIIADTHLFWPNGLTIDYAGRRMYWVDAKHHVIERANLDGSHRKAVISQGLPHPFAITVFEDSLYWTDWHTKSINSANKFTGKNQEIIRNKLHFPMDIHTLHPQRQPAGKNRCGDNNGGCTHLCLPSGQNYTCACPTGFRKISSHACAQSLDKFLLFARRMDIRRISFDTEDLSDDVIPLADVRSAVALDWDSRDDHVYWTDVSTDTISRAKWDGTGQEVVVDTSLESPAGLAIDWVTNKLYWTDAGTDRIEVANTDGSMRTVLIWENLDRPRDIVVEPMGGYMYWTDWGASPKIERAGMDASGRQVIISSNLTWPNGLAIDYGSQRLYWADAGMKTIEFAGLDGSKRKVLIGSQLPHPFGLTLYGERIYWTDWQTKSIQSADRLTGLDRETLQENLENLMDIHVFHRRRPPVSTPCAMENGGCSHLCLRSPNPSGFSCTCPTGINLLSDGKTCSPGMNSFLIFARRIDIRMVSLDIPYFADVVVPINITMKNTIAIGVDPQEGKVYWSDSTLHRISRANLDGSQHEDIITTGLQTTDGLAVDAIGRKVYWTDTGTNRIEVGNLDGSMRKVLVWQNLDSPRAIVLYHEMGFMYWTDWGENAKLERSGMDGSDRAVLINNNLGWPNGLTVDKASSQLLWADAHTERIEAADLNGANRHTLVSPVQHPYGLTLLDSYIYWTDWQTRSIHRADKGTGSNVILVRSNLPGLMDMQAVDRAQPLGFNKCGSRNGGCSHLCLPRPSGFSCACPTGIQLKGDGKTCDPSPETYLLFSSRGSIRRISLDTSDHTDVHVPVPELNNVISLDYDSVDGKVYYTDVFLDVIRRADLNGSNMETVIGRGLKTTDGLAVDWVARNLYWTDTGRNTIEASRLDGSCRKVLINNSLDEPRAIAVFPRKGYLFWTDWGHIAKIERANLDGSERKVLINTDLGWPNGLTLDYDTRRIYWVDAHLDRIESADLNGKLRQVLVSHVSHPFALTQQDRWIYWTDWQTKSIQRVDKYSGRNKETVLANVEGLMDIIVVSPQRQTGTNACGVNNGGCTHLCFARASDFVCACPDEPDSRPCSLVPGLVPPAPRATGMSEKSPVLPNTPPTTLYSSTTRTRTSLEEVEGRCSERDARLGLCARSNDAVPAAPGEGLHISYAIGGLLSILLILVVIAALMLYRHKKSKFTDPGMGNLTYSNPSYRTSTQEVKIEAIPKPAMYNQLCYKKEGGPDHNYTKEKIKIVEGICLLSGDDAEWDDLKQLRSSRGGLLRDHVCMKTDTVSIQASSGSLDDTETEQLLQEEQSECSSVHTAATPERRGSLPDTGWKHERKLSSESQV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
89
Chicken
89
Rabbit
89
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75096 As Substrate

Site PTM Type Enzyme
T689 Phosphorylation
S733 Phosphorylation
Y918 Phosphorylation
S935 Phosphorylation
T975 Phosphorylation
S1113 Phosphorylation
T1126 Phosphorylation
Y1484 Phosphorylation
T1512 Phosphorylation
T1688 O-Glycosylation
S1689 O-Glycosylation
K1753 Ubiquitination
Y1764 Phosphorylation
K1777 Ubiquitination
Y1787 Phosphorylation
Y1792 Phosphorylation
R1833 Methylation
S1887 Phosphorylation

Research Backgrounds

Function:

Mediates SOST-dependent inhibition of bone formation. Functions as a specific facilitator of SOST-mediated inhibition of Wnt signaling. Plays a key role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between motor neuron and skeletal muscle. Directly binds AGRIN and recruits it to the MUSK signaling complex. Mediates the AGRIN-induced phosphorylation of MUSK, the kinase of the complex. The activation of MUSK in myotubes induces the formation of NMJ by regulating different processes including the transcription of specific genes and the clustering of AChR in the postsynaptic membrane. Alternatively, may be involved in the negative regulation of the canonical Wnt signaling pathway, being able to antagonize the LRP6-mediated activation of this pathway. More generally, has been proposed to function as a cell surface endocytic receptor binding and internalizing extracellular ligands for degradation by lysosomes. May play an essential role in the process of digit differentiation (By similarity).

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in bone; present in osteoblasts and osteocytes. No expression is observed in osteoclast. Expressed in several regions of the brain.

Subunit Structure:

Homooligomer. Interacts with MUSK; the heterodimer forms an AGRIN receptor complex that binds AGRIN resulting in activation of MUSK (By similarity). Interacts (via the extracellular domain) with SOST; the interaction facilitates the inhibition of Wnt signaling. Interacts with MESD; the interaction promotes glycosylation of LRP4 and its cell-surface expression (By similarity).

Family&Domains:

Belongs to the LDLR family.

References

1). microRNA-136-5p from bone marrow mesenchymal stem cell-derived exosomes facilitates fracture healing by targeting LRP4 to activate the Wnt/β-catenin pathway. Bone & Joint Research, 2021 (PubMed: 34847690) [IF=4.6]

Application: WB    Species: Mouse    Sample: MC3T3-E1 cells

Fig. 4 MicroRNA (miR)-136-5p activates the Wnt/β-catenin pathway by targeting low-density lipoprotein receptor related protein 4 (LRP4). a) miR-136-5p binding sites in the LRP4 3’-untranslated region (3’-UTR) predicted by the TargetScan website, and binding of miR-136-5p to LRP4 confirmed by dual luciferase reporter gene assay in HEK293 cells; b) reverse transcription quantitative polymerase chain reaction (RT-qPCR) detection of the transfection efficiency of miR-136-5p mimic in MC3T3-E1 cells; c) RT-qPCR detection of the transfection efficiency of miR-136-5p inhibitor in MC3T3-E1 cells; d) Western blot detection of the protein expression of LRP4 in MC3T3-E1 cells with miR-136-5p overexpression or silencing; e) RT-qPCR detection of the expression of miR-136-5p in MC3T3-E1 cells transfected with miR-136-5p mimic, oe-LRP4, or both; f) Western blot detection of the expression of LRP4 and Wnt/β-catenin pathway related proteins in MC3T3-E1 cells transfected with miR-136-5p mimic, oe-LRP4, or both; g) RT-qPCR detected the expression of miR-136-5p in MC3T3-E1 cells treated with BMSC-Exos or in combination with miR-136-5p inhibitor; h) Western blot detection of the expression of LRP4 and Wnt/β-catenin pathway related proteins in MC3T3-E1 cells treated with BMSC-Exos or in combination with miR-136-5p inhibitor. # indicates p < 0.05, and non-significant (NS) represents p > 0.05. Measurement data were presented as mean and standard deviation. Unpaired t-test was used for data comparison between two groups while data comparisons among multiple groups were performed using one-way analysis of variance (ANOVA) with Tukey’s post hoc test. Data comparison among groups at different timepoints was performed by repeated measurement ANOVA with Bonferroni’s post hoc test. The experiment was repeated three times. MUT, mutant; PBS, phosphate-buffered saline; WT, wild type.
2). miR‐485's anti‐drug resistant epilepsy effects by regulating SV2A/PSD‐95 and targeting ABCC1 and neuronal signaling‐transduction proteins in hippocampus of rats. Brain and Behavior, 2021 (PubMed: 34291586) [IF=3.1]

Application: WB    Species: Rat    Sample: hippocampus tissue

FIGURE 6 Theexpressionofneuronalsignaling-transduction proteinsinhippocampusofDREratsat9weeksafterSE.(a)Western blottingbands;(b)barographsofexpressionlevels.*p<.05versusthe

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