GBP5 Antibody | Affinity Biosciences

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Western blot analysis GBP5 using COLO205 whole cell lysates.

Product:	GBP5 Antibody
Catalog:	DF9568
Description:	Rabbit polyclonal antibody to GBP5
Application:	WB
Reactivity:	Human
Mol.Wt.:	67 kDa; 67kD(Calculated).
Uniprot:	Q96PP8
RRID:	AB_2842764

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

GBP5 Antibody detects endogenous levels of total GBP5.

RRID:

AB_2842764
Cite Format: Affinity Biosciences Cat# DF9568, RRID:AB_2842764.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

GBP 5; GBP TA antigen; GBP-5; GBP-TA antigen; GBP5; GBP5_HUMAN; GTP binding protein 5; GTP-binding protein 5; Guanine nucleotide binding protein 5; Guanine nucleotide-binding protein 5; Guanylate binding protein 5; Guanylate-binding protein 5; UNQ2427/PRO4987;

Immunogens

Immunogen:

Uniprot:

Q96PP8(GBP5_HUMAN) >>Visit HPA database.

Gene(ID):

GBP5(115362)

Expression:

Q96PP8 GBP5_HUMAN:

Expressed in peripheral blood monocytes (at protein level).

Sequence:

Q96PP8 GBP5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MALEIHMSDPMCLIENFNEQLKVNQEALEILSAITQPVVVVAIVGLYRTGKSYLMNKLAGKNKGFSVASTVQSHTKGIWIWCVPHPNWPNHTLVLLDTEGLGDVEKADNKNDIQIFALALLLSSTFVYNTVNKIDQGAIDLLHNVTELTDLLKARNSPDLDRVEDPADSASFFPDLVWTLRDFCLGLEIDGQLVTPDEYLENSLRPKQGSDQRVQNFNLPRLCIQKFFPKKKCFIFDLPAHQKKLAQLETLPDDELEPEFVQQVTEFCSYIFSHSMTKTLPGGIMVNGSRLKNLVLTYVNAISSGDLPCIENAVLALAQRENSAAVQKAIAHYDQQMGQKVQLPMETLQELLDLHRTSEREAIEVFMKNSFKDVDQSFQKELETLLDAKQNDICKRNLEASSDYCSALLKDIFGPLEEAVKQGIYSKPGGHNLFIQKTEELKAKYYREPRKGIQAEEVLQKYLKSKESVSHAILQTDQALTETEKKKKEAQVKAEAEKAEAQRLAAIQRQNEQMMQERERLHQEQVRQMEIAKQNWLAEQQKMQEQQMQEQAAQLSTTFQAQNRSLLSELQHAQRTVNNDDPCVLL

PTMs - Q96PP8 As Substrate

Q96PP8

Site	PTM Type	Source
S52	Phosphorylation	Uniprot
S157	Phosphorylation	Uniprot
K226	Acetylation	Uniprot
K380	Ubiquitination	Uniprot
K389	Ubiquitination	Uniprot
K533	Ubiquitination	Uniprot
S565	Phosphorylation	Uniprot

Research Backgrounds

Q96PP8_GBP5_HUMAN

Function:

As an activator of NLRP3 inflammasome assembly, plays a role in innate immunity and inflammation. Promotes selective NLRP3 inflammasome assembly in response to microbial and soluble, but not crystalline, agents. Hydrolyzes GTP, but in contrast to other family members, does not produce GMP.

PTMs:

Isoprenylation is required for proper subcellular location.

Subcellular Location:

Cytoplasm. Golgi apparatus membrane>Lipid-anchor.

Tissue Specificity:

Expressed in peripheral blood monocytes (at protein level).

Subunit Structure:

Homodimer. Heterodimer with other family members, including GBP1, GBP2, GBP3 and GBP4. Dimerization is nucleotide-dependent; isoform 1 and isoform 2 form dimers when GTP-bound. GDP-bound isoform 2 is monomeric, while isoform 1 remains dimeric. Dimerization regulates subcellular location. May also form tetramers (dimer of dimers) in the presence of GTP. Interacts with NLRP3, possibly in its tetrameric form, and promotes PYCARD/ASC polymerization.

Family&Domains:

Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3-type GTPase family. GB1 subfamily.

Research Fields

· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)

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For Research Use Only.
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GBP5 Antibody - #DF9568