GNG13 Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using GNG13 Antibody.

DF9557 at 1/100 staining Rat muscle tissue by IHC-P.

DF9557 at 1/100 staining Rat skin tissue by IHC-P.

Product:	GNG13 Antibody
Catalog:	DF9557
Description:	Rabbit polyclonal antibody to GNG13
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Dog, Xenopus
Mol.Wt.:	8 kDa; 8kD(Calculated).
Uniprot:	Q9P2W3
RRID:	AB_2842753

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Xenopus(89%)

Clonality:

Polyclonal

Specificity:

GNG13 Antibody detects endogenous levels of total GNG13.

RRID:

AB_2842753
Cite Format: Affinity Biosciences Cat# DF9557, RRID:AB_2842753.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

G gamma subunit, clone:h2 35; G(gamma)13; GBG13_HUMAN; Gng13; Guanine nucleotide binding protein 13; Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-13; h2 35;

Immunogens

Immunogen:

Uniprot:

Q9P2W3(GBG13_HUMAN) >>Visit HPA database.

Gene(ID):

GNG13(51764)

Sequence:

Q9P2W3 GBG13_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEEWDVPQMKKEVESLKYQLAFQREMASKTIPELLKWIEDGIPKDPFLNPDLMKNNPWVEKGKCTIL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9P2W3 As Substrate

Q9P2W3

Site	PTM Type	Enzyme	Source
S28	Phosphorylation		Uniprot

Research Backgrounds

Q9P2W3_GBG13_HUMAN

Function:

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

Subcellular Location:

Cell membrane>Lipid-anchor>Cytoplasmic side.

Subunit Structure:

G proteins are composed of 3 units, alpha, beta and gamma.

Family&Domains:

Belongs to the G protein gamma family.

Research Fields

· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)

· Human Diseases > Substance dependence > Morphine addiction.

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling. (View pathway)

· Organismal Systems > Nervous system > Glutamatergic synapse.

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Nervous system > GABAergic synapse.

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Sensory system > Olfactory transduction.

· Organismal Systems > Sensory system > Taste transduction.

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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GNG13 Antibody - #DF9557