GNG12 Antibody | Affinity Biosciences

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Western blot analysis GNG12 using COS7 whole cell lysates.

Product:	GNG12 Antibody
Catalog:	DF9556
Description:	Rabbit polyclonal antibody to GNG12
Application:	WB IHC
Reactivity:	Human, Mouse, Monkey
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	8 kDa; 8kD(Calculated).
Uniprot:	Q9UBI6
RRID:	AB_2842752

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Monkey

Prediction:

Pig(88%), Bovine(88%), Horse(100%), Sheep(88%), Rabbit(100%), Dog(100%), Chicken(86%)

Clonality:

Polyclonal

Specificity:

GNG12 Antibody detects endogenous levels of total GNG12.

RRID:

AB_2842752
Cite Format: Affinity Biosciences Cat# DF9556, RRID:AB_2842752.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

G-protein gamma-12 subunit; GNG12; Guanine nucleotide binding protein (G protein) gamma 12; guanine nucleotide-binding protein G(I)/G(S)/G(O); Hypothetical protein DKFZp762E193;

Immunogens

Immunogen:

Uniprot:

Q9UBI6(GBG12_HUMAN) >>Visit HPA database.

Gene(ID):

GNG12(55970)

Sequence:

Q9UBI6 GBG12_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSSKTASTNNIAQARRTVQQLRLEASIERIKVSKASADLMSYCEEHARSDPLLIGIPTSENPFKDKKTCIIL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Dog

100

Rabbit

100

Pig

Bovine

Sheep

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UBI6 As Substrate

Q9UBI6

Site	PTM Type	Source
S2	Acetylation	Uniprot
S2	Phosphorylation	Uniprot
K4	Ubiquitination	Uniprot
T5	Phosphorylation	Uniprot
S7	Phosphorylation	Uniprot
T8	Phosphorylation	Uniprot
S26	Phosphorylation	Uniprot
K34	Ubiquitination	Uniprot
S41	Phosphorylation	Uniprot
Y42	Phosphorylation	Uniprot
C43	S-Nitrosylation	Uniprot
S49	Phosphorylation	Uniprot
K64	Ubiquitination	Uniprot

Research Backgrounds

Q9UBI6_GBG12_HUMAN

Function:

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

Subcellular Location:

Cell membrane>Lipid-anchor>Cytoplasmic side.

Subunit Structure:

G proteins are composed of 3 units, alpha, beta and gamma.

Family&Domains:

Belongs to the G protein gamma family.

Research Fields

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)

· Human Diseases > Substance dependence > Morphine addiction.

· Human Diseases > Substance dependence > Alcoholism.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling. (View pathway)

· Organismal Systems > Nervous system > Glutamatergic synapse.

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Nervous system > GABAergic synapse.

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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GNG12 Antibody - #DF9556