G-CSF Antibody | Affinity Biosciences

WB
IHC
Cites

1/4

Western blot analysis of CSF3 using Jurkat whole cell lysates.

DF9542 at 1/100 staining Human pancreatic cancer and adjacent nomal tissues by IHC-P.

DF9542 at 1/100 staining Rat kidney tissue by IHC-P.

$FIGURE 4 Transcriptome heterogeneity of different subsets of Kupffer cells. (A) t- SNE plots of the expression of representative marker genes for Kupffer cells. (B) UMAP plot of Kupffer cells by clusters (up) and group (down). CDL, control donor liver; FDL, fatty donor liver. (C) Violin plot showing the expression of marker genes in the indicated clusters of Kupffer cells. The colourful bars corresponding to specific clusters in (b). (D) Volcano plot shows differentially expressed genes between CDL (blue dots) and FDL Kupffer cells (red dots). The names of some significant genes are indicated in the plots. (E) The KEGG pathway enrichment analysis of differentially expressed genes (DEGs) among Kupffer cell subtypes. (F) Multiplexed immunofluorescence staining to validate the existence of CSF3 + Kupffer cells (KCs) in FDL sample, white arrows (CLEC4F + CSF3 + ). Scale bar, 20 μm. (G) Connection graph showing the intensity of interactions between one cell type to another in coloured circles. Interactions were evaluated among all cell types, including immune and non- immune fractions. The node size indicated the number of L- R interactions$

FIGURE 4 Transcriptome heterogeneity of different subsets of Kupffer cells.

Product:	G-CSF Antibody
Catalog:	DF9542
Description:	Rabbit polyclonal antibody to G-CSF
Application:	WB IHC
Reactivity:	Human, Rat
Prediction:	Bovine, Sheep, Rabbit
Mol.Wt.:	22 kDa; 22kD(Calculated).
Uniprot:	P09919
RRID:	AB_2842738

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Prediction:

Bovine(83%), Sheep(83%), Rabbit(82%)

Clonality:

Polyclonal

Specificity:

G-CSF Antibody detects endogenous levels of total G-CSF.

RRID:

AB_2842738
Cite Format: Affinity Biosciences Cat# DF9542, RRID:AB_2842738.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

C17orf33; Colony stimulating factor 3 (granulocyte); Colony stimulating factor 3; CSF 3; CSF beta; CSF3; CSF3_HUMAN; CSF3OS; Csfg; Filgrastim; G-CSF; GCSA; GCSF; Granulocyte colony stimulating factor; Granulocyte colony-stimulating factor; Lenograstim; Macrophage granulocyte inducer 2; MGC45931; MGI 2; Pluripoietin;

Immunogens

Immunogen:

Uniprot:

P09919(CSF3_HUMAN) >>Visit HPA database.

Gene(ID):

CSF3(1440)

Sequence:

P09919 CSF3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAGPATQSPMKLMALQLLLWHSALWTVQEATPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLVSECATYKLCHPEELVLLGHSLGIPWAPLSSCPSQALQLAGCLSQLHSGLFLYQGLLQALEGISPELGPTLDTLQLDVADFATTIWQQMEELGMAPALQPTQGAMPAFASAFQRRAGGVLVASHLQSFLEVSYRVLRHLAQP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

Sheep

Rabbit

Dog

Pig

Horse

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P09919 As Substrate

P09919

Site	PTM Type	Enzyme	Source
T6	Phosphorylation		Uniprot

Research Backgrounds

P09919_CSF3_HUMAN

Function:

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

PTMs:

O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).

Subcellular Location:

Secreted.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the IL-6 superfamily.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Malaria.

· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)

References

1). Single‐cell profiling reveals distinct immune phenotypes that contribute to ischaemia‐reperfusion injury after steatotic liver transplantation. Cell Proliferation, 2021 (PubMed: 34469018) [IF=8.5]

Application: IF/ICC Species: Rat Sample: Kupffer cells

FIGURE 4 Transcriptome heterogeneity of different subsets of Kupffer cells. (A) t- SNE plots of the expression of representative marker genes for Kupffer cells. (B) UMAP plot of Kupffer cells by clusters (up) and group (down). CDL, control donor liver; FDL, fatty donor liver. (C) Violin plot showing the expression of marker genes in the indicated clusters of Kupffer cells. The colourful bars corresponding to specific clusters in (b). (D) Volcano plot shows differentially expressed genes between CDL (blue dots) and FDL Kupffer cells (red dots). The names of some significant genes are indicated in the plots. (E) The KEGG pathway enrichment analysis of differentially expressed genes (DEGs) among Kupffer cell subtypes. (F) Multiplexed immunofluorescence staining to validate the existence of CSF3 + Kupffer cells (KCs) in FDL sample, white arrows (CLEC4F + CSF3 + ). Scale bar, 20 μm. (G) Connection graph showing the intensity of interactions between one cell type to another in coloured circles. Interactions were evaluated among all cell types, including immune and non- immune fractions. The node size indicated the number of L- R interactions

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G-CSF Antibody - #DF9542