GPSM1 Antibody - #DF9539

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Product: GPSM1 Antibody
Catalog: DF9539
Description: Rabbit polyclonal antibody to GPSM1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Sheep
Mol.Wt.: 75 kDa; 75kD(Calculated).
Uniprot: Q86YR5
RRID: AB_2842735

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(90%), Sheep(90%)
Clonality:
Polyclonal
Specificity:
GPSM1 Antibody detects endogenous levels of total GPSM1.
RRID:
AB_2842735
Cite Format: Affinity Biosciences Cat# DF9539, RRID:AB_2842735.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Activator of G-protein signaling 3; AGS3; AGS3 homolog; C10a; G protein signaling modulator 1 (AGS3 like, C. elegans); G-protein-signaling modulator 1; GPSM1; GPSM1_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q86YR5(GPSM1_HUMAN) >>Visit HPA database.

Gene(ID):
GPSM1(26086)
Expression:
Q86YR5 GPSM1_HUMAN:

Expressed in intestinal cells.

Sequence:
MAGPAPPVADELPGPAARRLYSRMEASCLELALEGERLCKAGDFKTGVAFFEAAVQVGTEDLKTLSAIYSQLGNAYFYLKEHGRALEYHKHDLLLARTIGDRMGEAKASGNLGNTLKVLGRFDEAAVCCQRHLSIAQEQGDKVGEARALYNIGNVYHAKGKQLSWNAANATQDPGHLPPDVRETLCKASEFYERNLSLVKELGDRAAQGRAYGNLGNTHYLLGNFTEATTFHKERLAIAKEFGDKAAERRAYSNLGNAHVFLGRFDVAAEYYKKTLQLSRQLRDQAVEAQACYSLGNTYTLLQDYERAAEYHLRHLLIAQELADRVGEGRACWSLGNAYVSMGRPAQALTFAKKHLQISQEIGDRHGELTARMNVAQLQLVLGRLTSPAASEKPDLAGYEAQGARPKRTQRLSAETWDLLRLPLEREQNGDSHHSGDWRGPSRDSLPLPVRSRKYQEGPDAERRPREGSHSPLDSADVRVHVPRTSIPRAPSSDEECFFDLLTKFQSSRMDDQRCPLDDGQAGAAEATAAPTLEDRIAQPSMTASPQTEEFFDLIASSQSRRLDDQRASVGSLPGLRITHSNAGHLRGHGEPQEPGDDFFNMLIKYQSSRIDDQRCPPPDVLPRGPTMPDEDFFSLIQRVQAKRMDEQRVDLAGGPEQGAGGPPEPQQQCQPGAS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
90
Sheep
90
Dog
70
Horse
67
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q86YR5 As Substrate

Site PTM Type Enzyme
K40 Ubiquitination
K45 Ubiquitination
K90 Ubiquitination
K107 Ubiquitination
K117 Ubiquitination
S134 Phosphorylation
K142 Ubiquitination
Y150 Phosphorylation
Y156 Phosphorylation
K161 Ubiquitination
K187 Ubiquitination
K200 Ubiquitination
K233 Ubiquitination
K240 Ubiquitination
K245 Ubiquitination
Y252 Phosphorylation
K274 Ubiquitination
Y311 Phosphorylation
R325 Methylation
K354 Ubiquitination
S387 Phosphorylation
K393 Ubiquitination
S413 Phosphorylation
R421 Methylation
S445 Phosphorylation
S469 Phosphorylation
S471 Phosphorylation
R479 Methylation
S486 Phosphorylation
S492 Phosphorylation
S493 Phosphorylation
K504 Ubiquitination
C515 S-Nitrosylation
S541 Phosphorylation
S545 Phosphorylation
T548 Phosphorylation
S569 Phosphorylation
S572 Phosphorylation
C670 S-Nitrosylation

Research Backgrounds

Function:

Guanine nucleotide dissociation inhibitor (GDI) which functions as a receptor-independent activator of heterotrimeric G-protein signaling. Keeps G(i/o) alpha subunit in its GDP-bound form thus uncoupling heterotrimeric G-proteins signaling from G protein-coupled receptors. Controls spindle orientation and asymmetric cell fate of cerebral cortical progenitors. May also be involved in macroautophagy in intestinal cells. May play a role in drug addiction.

PTMs:

Phosphorylation regulates interaction with G(i/o) alpha.

Subcellular Location:

Cytoplasm>Cytosol. Endoplasmic reticulum membrane>Peripheral membrane protein>Cytoplasmic side. Golgi apparatus membrane>Peripheral membrane protein>Cytoplasmic side. Cell membrane>Peripheral membrane protein>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in intestinal cells.

Subunit Structure:

Interacts with GNAI1, GNAI2 and GNAI3 preferentially in their GDP-bound state. May also interact with GNAO1. Interacts with STK11/LKB1 and MACF1 (By similarity). Interacts with INSC/inscuteable and FRMPD1.

Family&Domains:

The GoLoco domains mediate interaction with G(i/o) alpha (By similarity). The GoLoco domains are essential for the GDI activity toward G(i/o) alpha.

Belongs to the GPSM family.

Research Fields

· Human Diseases > Substance dependence > Cocaine addiction.

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