GAPDHS Antibody | Affinity Biosciences

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Western blot analysis of extracts from COS-7, using GAPDHS Antibody.

Product:	GAPDHS Antibody
Catalog:	DF9535
Description:	Rabbit polyclonal antibody to GAPDHS
Application:	WB
Reactivity:	Human, Monkey
Prediction:	Zebrafish, Horse, Dog
Mol.Wt.:	46 kDa,55kDa; 45kD(Calculated).
Uniprot:	O14556
RRID:	AB_2842731

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Monkey

Prediction:

Zebrafish(89%), Horse(83%), Dog(92%)

Clonality:

Polyclonal

Specificity:

GAPDHS Antibody detects endogenous levels of total GAPDHS.

RRID:

AB_2842731
Cite Format: Affinity Biosciences Cat# DF9535, RRID:AB_2842731.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

G3PT_HUMAN; GAPD2; GAPDH 2; GAPDH-2; GAPDH2; GAPDHS; GAPDS; Glyceraldehyde 3 phosphate dehydrogenase spermatogenic; Glyceraldehyde 3 phosphate dehydrogenase, testis specific; Glyceraldehyde-3-phosphate dehydrogenase; HSD 35; HSD35; Spermatogenic cell specific glyceraldehyde 3 phosphate dehydrogenase 2; Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde 3 phosphate dehydrogenase; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase; testis-specific;

Immunogens

Immunogen:

Uniprot:

O14556(G3PT_HUMAN) >>Visit HPA database.

Gene(ID):

GAPDHS(26330)

Expression:

O14556 G3PT_HUMAN:

Testis specific.

Sequence:

O14556 G3PT_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSKRDIVLTNVTVVQLLRQPCPVTRAPPPPEPKAEVEPQPQPEPTPVREEIKPPPPPLPPHPATPPPKMVSVARELTVGINGFGRIGRLVLRACMEKGVKVVAVNDPFIDPEYMVYMFKYDSTHGRYKGSVEFRNGQLVVDNHEISVYQCKEPKQIPWRAVGSPYVVESTGVYLSIQAASDHISAGAQRVVISAPSPDAPMFVMGVNENDYNPGSMNIVSNASCTTNCLAPLAKVIHERFGIVEGLMTTVHSYTATQKTVDGPSRKAWRDGRGAHQNIIPASTGAAKAVTKVIPELKGKLTGMAFRVPTPDVSVVDLTCRLAQPAPYSAIKEAVKAAAKGPMAGILAYTEDEVVSTDFLGDTHSSIFDAKAGIALNDNFVKLISWYDNEYGYSHRVVDLLRYMFSRDK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

Zebrafish

Horse

Pig

Bovine

Rabbit

Sheep

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O14556 As Substrate

O14556

Site	PTM Type	Enzyme	Source
T77	Phosphorylation		Uniprot
T301	Phosphorylation		Uniprot

Research Backgrounds

O14556_G3PT_HUMAN

Function:

May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility (By similarity).

Subcellular Location:

Cytoplasm.

Tissue Specificity:

Testis specific.

Subunit Structure:

Homotetramer. Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation.

Family&Domains:

The testis-specific N-terminal extension mediates tight association with the cytoskeletal fibrous sheath of the spermatozoa flagellum, possibly via interchain disulfide-bonding of Cys-21 with sheath components.

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Research Fields

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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GAPDHS Antibody - #DF9535