FGF9 Antibody - #DF9532

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Product: FGF9 Antibody
Catalog: DF9532
Description: Rabbit polyclonal antibody to FGF9
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 23 kDa; 23kD(Calculated).
Uniprot: P31371
RRID: AB_2842728

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(95%), Horse(100%), Sheep(95%), Rabbit(100%), Dog(100%), Chicken(90%), Xenopus(86%)
Clonality:
Polyclonal
Specificity:
FGF9 Antibody detects endogenous levels of total FGF9.
RRID:
AB_2842728
Cite Format: Affinity Biosciences Cat# DF9532, RRID:AB_2842728.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FGF 9; FGF-9; FGF9; FGF9_HUMAN; Fibroblast growth factor 9; GAF (Glia-activafibroblast growth factor 9 (glia-activating factor); GAF; Glia Activating Factor; Glia-activating factor; HBFG 9; HBFG9; HBGF-9; Heparin-binding growth factor 9; MGC119914; MGC119915; SYNS3;

Immunogens

Immunogen:
Uniprot:

P31371(FGF9_HUMAN) >>Visit HPA database.

Gene(ID):
FGF9(2254)
Expression:
P31371 FGF9_HUMAN:

Glial cells.

Sequence:
MAPLGEVGNYFGVQDAVPFGNVPVLPVDSPVLLSDHLGQSEAGGLPRGPAVTDLDHLKGILRRRQLYCRTGFHLEIFPNGTIQGTRKDHSRFGILEFISIAVGLVSIRGVDSGLYLGMNEKGELYGSEKLTQECVFREQFEENWYNTYSSNLYKHVDTGRRYYVALNKDGTPREGTRTKRHQKFTHFLPRPVDPDKVPELYKDILSQS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Rabbit
100
Bovine
95
Sheep
95
Chicken
90
Xenopus
86
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P31371 As Substrate

Site PTM Type Enzyme
Y163 Phosphorylation
T176 Phosphorylation
T178 Phosphorylation

Research Backgrounds

Function:

Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.

PTMs:

Three molecular species were found (30 kDa, 29 kDa and 25 kDa), cleaved at Leu-4, Val-13 and Ser-34 respectively. The smaller ones might be products of proteolytic digestion. Furthermore, there may be a functional signal sequence in the 30 kDa species which is uncleavable in the secretion step.

N-glycosylated.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Glial cells.

Subunit Structure:

Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors.

Family&Domains:

Belongs to the heparin-binding growth factors family.

Research Fields

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

References

1). Role of FGF9 in sheep testis steroidogenesis during sexual maturation. ANIMAL REPRODUCTION SCIENCE, 2018 (PubMed: 30154034) [IF=2.2]

Application: IHC    Species: sheep    Sample: testis

Fig. 2. |Concentrations of serum testosterone and expressions of FGF9 in testis of male sheep at different development stages. A, concentrations of serum testosterone by ELISA. B, relative abundances of FGF9 mRNA. C and D, abundances of FGF9 proteins. E, FGF9-immunopositive cells in the testis

Application: WB    Species: sheep    Sample: testis

Fig. 2. |Concentrations of serum testosterone and expressions of FGF9 in testis of male sheep at different development stages. A, concentrations of serum testosterone by ELISA. B, relative abundances of FGF9 mRNA. C and D, abundances of FGF9 proteins. E, FGF9-immunopositive cells in the testis

Application: IF/ICC    Species: sheep    Sample: Leydig cells

Fig. 3. | Abundances of FGF9 in Leydig cells after siRNA interference. A, analysis of FGF9 immunoreactivity in Leydig cells. B, analysis of relative abundances of FGF9 mRNA by qRT-PCR. C, analysis of the abundances of FGF9 protein by western blotting; All data are presented as means ± SD from at least three separate experiments; Different letters indicate differences between means (P < 0.05); Bar = 50 μm

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