GABRR1 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis GABRR1 using COLO205 whole cell lysates.

Product:	GABRR1 Antibody
Catalog:	DF9525
Description:	Rabbit polyclonal antibody to GABRR1
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	56 kDa; 56kD(Calculated).
Uniprot:	P24046
RRID:	AB_2842721

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%)

Clonality:

Polyclonal

Specificity:

GABRR1 Antibody detects endogenous levels of total GABRR1.

RRID:

AB_2842721
Cite Format: Affinity Biosciences Cat# DF9525, RRID:AB_2842721.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

bA135P14.1 (gamma-aminobutyric acid (GABA) receptor rho 1); GABA(A) receptor subunit rho-1; GABA(C) receptor; GABRR1; gamma aminobutyric acid receptor rho 1; gamma-aminobutyric acid (GABA) A receptor rho-1; gamma-aminobutyric acid (GABA) receptor rho 1; Gamma-aminobutyric acid receptor subunit rho-1; GBRR1_HUMAN; MGC163216;

Immunogens

Immunogen:

Uniprot:

P24046(GBRR1_HUMAN) >>Visit HPA database.

Gene(ID):

GABRR1(2569)

Expression:

P24046 GBRR1_HUMAN:

Highly expressed in the retina and in a lesser extent in brain, lung and thymus.

Sequence:

P24046 GBRR1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLAVPNMRFGIFLLWWGWVLATESRMHWPGREVHEMSKKGRPQRQRREVHEDAHKQVSPILRRSPDITKSPLTKSEQLLRIDDHDFSMRPGFGGPAIPVGVDVQVESLDSISEVDMDFTMTLYLRHYWKDERLSFPSTNNLSMTFDGRLVKKIWVPDMFFVHSKRSFIHDTTTDNVMLRVQPDGKVLYSLRVTVTAMCNMDFSRFPLDTQTCSLEIESYAYTEDDLMLYWKKGNDSLKTDERISLSQFLIQEFHTTTKLAFYSSTGWYNRLYINFTLRRHIFFFLLQTYFPATLMVMLSWVSFWIDRRAVPARVPLGITTVLTMSTIITGVNASMPRVSYIKAVDIYLWVSFVFVFLSVLEYAAVNYLTTVQERKEQKLREKLPCTSGLPPPRTAMLDGNYSDGEVNDLDNYMPENGEKPDRMMVQLTLASERSSPQRKSQRSSYVSMRIDTHAIDKYSRIIFPAAYILFNLIYWSIFS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Rabbit

100

Xenopus

Zebrafish

Pig

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P24046 As Substrate

P24046

Site	PTM Type	Source
S134	Phosphorylation	Uniprot
S166	Phosphorylation	Uniprot
T171	Phosphorylation	Uniprot
T172	Phosphorylation	Uniprot
Y219	Phosphorylation	Uniprot
Y221	Phosphorylation	Uniprot
Y229	Phosphorylation	Uniprot
S431	Phosphorylation	Uniprot

Research Backgrounds

P24046_GBRR1_HUMAN

Function:

GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. Rho-1 GABA receptor could play a role in retinal neurotransmission.

Subcellular Location:

Cell junction>Synapse>Postsynaptic cell membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Highly expressed in the retina and in a lesser extent in brain, lung and thymus.

Subunit Structure:

Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho. Interacts with SQSTM1 (By similarity).

Family&Domains:

Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRR1 sub-subfamily.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Substance dependence > Morphine addiction.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling. (View pathway)

· Organismal Systems > Nervous system > GABAergic synapse.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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GABRR1 Antibody - #DF9525