FOLR3 Antibody | Affinity Biosciences

IHC
Cites

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DF9519 at 1/100 staining human ovarian cancer by IHC-P.

Product:	FOLR3 Antibody
Catalog:	DF9519
Description:	Rabbit polyclonal antibody to FOLR3
Application:	WB IHC
Reactivity:	Human, Mouse
Mol.Wt.:	25,37 kDa; 28kD(Calculated).
Uniprot:	P41439
RRID:	AB_2842715

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

FOLR3 Antibody detects endogenous levels of total FOLR3.

RRID:

AB_2842715
Cite Format: Affinity Biosciences Cat# DF9519, RRID:AB_2842715.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Folate receptor 3 (gamma); Folate receptor 3; Folate receptor gamma; FOLR3; FOLR3_HUMAN; FR G; FR-gamma; Gamma hFR;

Immunogens

Immunogen:

Uniprot:

P41439(FOLR3_HUMAN) >>Visit HPA database.

Gene(ID):

FOLR3(2352)

Expression:

P41439 FOLR3_HUMAN:

Spleen, thymus, bone marrow, ovarian carcinoma, and uterine carcinoma.

Sequence:

P41439 FOLR3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDMAWQMMQLLLLALVTAAGSAQPRSARARTDLLNVCMNAKHHKTQPSPEDELYGQCSPWKKNACCTASTSQELHKDTSRLYNFNWDHCGKMEPTCKRHFIQDSCLYECSPNLGPWIRQVNQSWRKERILNVPLCKEDCERWWEDCRTSYTCKSNWHKGWNWTSGINECPAGALCSTFESYFPTPAALCEGLWSHSFKVSNYSRGSGRCIQMWFDSAQGNPNEEVAKFYAAAMNAGAPSRGIIDS

PTMs - P41439 As Substrate

P41439

Site	PTM Type	Enzyme	Source
S194	Phosphorylation		Uniprot
S196	Phosphorylation		Uniprot

Research Backgrounds

P41439_FOLR3_HUMAN

Function:

Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. Isoform Short does not bind folate.

Subcellular Location:

Secreted.

Tissue Specificity:

Spleen, thymus, bone marrow, ovarian carcinoma, and uterine carcinoma.

Family&Domains:

Belongs to the folate receptor family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.

References

1). Reactive oxygen species-responsive dexamethasone-loaded nanoparticles for targeted treatment of rheumatoid arthritis via suppressing the iRhom2/TNF-α/BAFF signaling pathway. Biomaterials, 2020 (PubMed: 31918224) [IF=14.0]

Application: WB Species: Mouse Sample: Raw264.7 and MH7A cells

Fig. 3. In vitro cellular uptake of Cy5-labeled NPs. (A) Raw264.7 macrophages induced with LPS (1 μg/mL) for 6 h. Then cellular uptake of Cy5-labeled Dex/Oxi- αCD NPs or Dex/FA-Oxi-αCD NPs was assessed using confocal laser scanning microscopy at various times. Nuclei were counterstained with DAPI (blue). Scale bars, 20 μm. (B) Quantitative analysis of the corresponding fluorescence intensity of intracellular NPs (red) in Raw264.7 cells. (C) The mRNA expression of FRα, FRβ, and FRγ in MH7A cells were analyzed by quantitative real-time PCR. Data were presented by the relative amount of mRNA normalized by GAPDH. (D) Western blot analysis of FRα, FRβ and FRγ expression in Raw264.7 and MH7A cells. α-Tubulin was used as a loading control. (E–F) Cellular uptake (E) and corresponding fluorescence intensity (F) of Cy5-labeled Dex/Oxi-NPs or Dex/FA-Oxi-NPs in MH7A cells at various time points. *p < 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

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FOLR3 Antibody - #DF9519