EIF5 Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using EIF5 Antibody.

Product:	EIF5 Antibody
Catalog:	DF9511
Description:	Rabbit polyclonal antibody to EIF5
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Dog, Xenopus
Mol.Wt.:	49 kDa; 49kD(Calculated).
Uniprot:	P55010
RRID:	AB_2842707

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

EIF5 Antibody detects endogenous levels of total EIF5.

RRID:

AB_2842707
Cite Format: Affinity Biosciences Cat# DF9511, RRID:AB_2842707.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

2810011H21Rik; D12Ertd549e; EIF 5; EIF 5A; eIF-5; Eif5; Eukaryotic initiation factor 5; Eukaryotic translation initiation factor 5; IF5_HUMAN; MGC36374; MGC36509;

Immunogens

Immunogen:

Uniprot:

P55010(IF5_HUMAN) >>Visit HPA database.

Gene(ID):

EIF5(1983)

Sequence:

P55010 IF5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSVNVNRSVSDQFYRYKMPRLIAKVEGKGNGIKTVIVNMVDVAKALNRPPTYPTKYFGCELGAQTQFDVKNDRYIVNGSHEANKLQDMLDGFIKKFVLCPECENPETDLHVNPKKQTIGNSCKACGYRGMLDTHHKLCTFILKNPPENSDSGTGKKEKEKKNRKGKDKENGSVSSSETPPPPPPPNEINPPPHTMEEEEDDDWGEDTTEEAQRRRMDEISDHAKVLTLSDDLERTIEERVNILFDFVKKKKEEGVIDSSDKEIVAEAERLDVKAMGPLVLTEVLFNEKIREQIKKYRRHFLRFCHNNKKAQRYLLHGLECVVAMHQAQLISKIPHILKEMYDADLLEEEVIISWSEKASKKYVSKELAKEIRVKAEPFIKWLKEAEEESSGGEEEDEDENIEVVYSKAASVPKVETVKSDNKDDDIDIDAI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P55010 As Substrate

P55010

Site	PTM Type	Enzyme	Source
S2	Phosphorylation		Uniprot
S8	Phosphorylation		Uniprot
S10	Phosphorylation		Uniprot
Y14	Phosphorylation		Uniprot
Y16	Phosphorylation		Uniprot
K24	Acetylation		Uniprot
K24	Ubiquitination		Uniprot
K28	Acetylation		Uniprot
K33	Acetylation		Uniprot
K55	Acetylation		Uniprot
K55	Ubiquitination		Uniprot
K70	Ubiquitination		Uniprot
K95	Ubiquitination		Uniprot
K114	Ubiquitination		Uniprot
K115	Ubiquitination		Uniprot
S121	Phosphorylation		Uniprot
T139	Phosphorylation		Uniprot
S149	Phosphorylation		Uniprot
S151	Phosphorylation		Uniprot
S174	Phosphorylation	P67870 (CSNK2B) , P68400 (CSNK2A1)	Uniprot
S175	Phosphorylation		Uniprot
S176	Phosphorylation		Uniprot
T178	Phosphorylation		Uniprot
T207	Phosphorylation	P68400 (CSNK2A1)	Uniprot
T208	Phosphorylation	P68400 (CSNK2A1)	Uniprot
S220	Phosphorylation		Uniprot
T227	Phosphorylation		Uniprot
S229	Phosphorylation		Uniprot
K261	Ubiquitination		Uniprot
K288	Ubiquitination		Uniprot
Y362	Phosphorylation		Uniprot
K369	Acetylation		Uniprot
S389	Phosphorylation	P67870 (CSNK2B) , P68400 (CSNK2A1)	Uniprot
S390	Phosphorylation	P68400 (CSNK2A1) , P67870 (CSNK2B)	Uniprot
Y405	Phosphorylation		Uniprot
S406	Phosphorylation		Uniprot
S410	Phosphorylation		Uniprot
T416	Phosphorylation		Uniprot
S419	Phosphorylation		Uniprot

Research Backgrounds

P55010_IF5_HUMAN

Function:

Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).

Subunit Structure:

Interacts with FMR1 isoform 6; this interaction occurs in a RNA-dependent manner.

Family&Domains:

Belongs to the eIF-2-beta/eIF-5 family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

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EIF5 Antibody - #DF9511