HSD17B12 Antibody - #DF9505
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF9505, RRID:AB_2842701.
Fold/Unfold
12,3-ketoacyl-CoA reductase,short chain; 17 beta HSD 12; 17 beta hydroxysteroid dehydrogenase 12; 17-@beta-HSD XII; 17-@beta-hydroxysteroid dehydrogenase XII; 17-beta-HSD 12; 17-beta-hydroxysteroid dehydrogenase 12; 17beta HSD type 12; 3 ketoacyl CoA reductase; 3-ketoacyl-CoA reductase; DHB12_HUMAN; Estradiol 17 beta dehydrogenase 12; Estradiol 17-beta-dehydrogenase 12; HSD17B12; Hydroxysteroid (17-beta) dehydrogenase 12; KAR; KIK-I; Kik1; SDR12C1; Short chain dehydrogenase/reductase family 12C member 1; Steroid dehydrogenase homolog;
Immunogens
Expressed in most tissues tested. Highly expressed in the ovary and mammary. Expressed in platelets.
- Q53GQ0 DHB12_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MESALPAAGFLYWVGAGTVAYLALRISYSLFTALRVWGVGNEAGVGPGLGEWAVVTGSTDGIGKSYAEELAKHGMKVVLISRSKDKLDQVSSEIKEKFKVETRTIAVDFASEDIYDKIKTGLAGLEIGILVNNVGMSYEYPEYFLDVPDLDNVIKKMININILSVCKMTQLVLPGMVERSKGAILNISSGSGMLPVPLLTIYSATKTFVDFFSQCLHEEYRSKGVFVQSVLPYFVATKLAKIRKPTLDKPSPETFVKSAIKTVGLQSRTNGYLIHALMGSIISNLPSWIYLKIVMNMNKSTRAHYLKKTKKN
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q53GQ0 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y12 | Phosphorylation | Uniprot | |
| Y66 | Phosphorylation | Uniprot | |
| K72 | Acetylation | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| K86 | Ubiquitination | Uniprot | |
| S91 | Phosphorylation | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| S111 | Phosphorylation | Uniprot | |
| Y115 | Phosphorylation | Uniprot | |
| K117 | Acetylation | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| T169 | Phosphorylation | Uniprot | |
| K181 | Ubiquitination | Uniprot | |
| C215 | S-Nitrosylation | Uniprot | |
| K223 | Ubiquitination | Uniprot | |
| Y233 | Phosphorylation | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| K241 | Ubiquitination | Uniprot | |
| K244 | Ubiquitination | Uniprot | |
| S251 | Phosphorylation | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| K261 | Ubiquitination | Uniprot | |
| K299 | Ubiquitination | Uniprot |
Research Backgrounds
Catalyzes the second of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme has a 3-ketoacyl-CoA reductase activity, reducing 3-ketoacyl-CoA to 3-hydroxyacyl-CoA, within each cycle of fatty acid elongation. Thereby, it may participate in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. May also catalyze the transformation of estrone (E1) into estradiol (E2) and play a role in estrogen formation.
Endoplasmic reticulum membrane>Multi-pass membrane protein.
Expressed in most tissues tested. Highly expressed in the ovary and mammary. Expressed in platelets.
Interacts with ELOVL1 and LASS2.
The di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins.
Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.
Research Fields
· Metabolism > Lipid metabolism > Fatty acid elongation.
· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.
· Metabolism > Lipid metabolism > Biosynthesis of unsaturated fatty acids.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Fatty acid metabolism.
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