EDEM1 Antibody | Affinity Biosciences

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Western blot analysis of EDEM1 using NIH-3T3 whole cell lysates.

Product:	EDEM1 Antibody
Catalog:	DF9502
Description:	Rabbit polyclonal antibody to EDEM1
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	74 kDa; 74kD(Calculated).
Uniprot:	Q92611
RRID:	AB_2842698

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(89%), Horse(100%), Sheep(89%), Rabbit(89%), Dog(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

EDEM1 Antibody detects endogenous levels of total EDEM1.

RRID:

AB_2842698
Cite Format: Affinity Biosciences Cat# DF9502, RRID:AB_2842698.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

EDEM 1; EDEM; Endoplasmic reticulum degradation enhancing alpha mannosidase like protein; ER degradation enhancer, mannosidase alpha like 1; ER degradation enhancing alpha mannosidase like 1; ER degradation enhancing alpha-mannosidase like protein 1; KIAA0212;

Immunogens

Immunogen:

Uniprot:

Q92611(EDEM1_HUMAN) >>Visit HPA database.

Gene(ID):

EDEM1(9695)

Sequence:

Q92611 EDEM1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MQWRALVLGLVLLRLGLHGVLWLVFGLGPSMGFYQRFPLSFGFQRLRSPDGPASPTSGPVGRPGGVSGPSWLQPPGTGAAQSPRKAPRRPGPGMCGPANWGYVLGGRGRGPDEYEKRYSGAFPPQLRAQMRDLARGMFVFGYDNYMAHAFPQDELNPIHCRGRGPDRGDPSNLNINDVLGNYSLTLVDALDTLAIMGNSSEFQKAVKLVINTVSFDKDSTVQVFEATIRVLGSLLSAHRIITDSKQPFGDMTIKDYDNELLYMAHDLAVRLLPAFENTKTGIPYPRVNLKTGVPPDTNNETCTAGAGSLLVEFGILSRLLGDSTFEWVARRAVKALWNLRSNDTGLLGNVVNIQTGHWVGKQSGLGAGLDSFYEYLLKSYILFGEKEDLEMFNAAYQSIQNYLRRGREACNEGEGDPPLYVNVNMFSGQLMNTWIDSLQAFFPGLQVLIGDVEDAICLHAFYYAIWKRYGALPERYNWQLQAPDVLFYPLRPELVESTYLLYQATKNPFYLHVGMDILQSLEKYTKVKCGYATLHHVIDKSTEDRMESFFLSETCKYLYLLFDEDNPVHKSGTRYMFTTEGHIVSVDEHLRELPWKEFFSEEGGQDQGGKSVHRPKPHELKVINSSSNCNRVPDERRYSLPLKSIYMRQIDQMVGLI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Dog

100

Chicken

100

Bovine

Sheep

Rabbit

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92611 As Substrate

Q92611

Site	PTM Type	Source
K116	Ubiquitination	Uniprot
K279	Ubiquitination	Uniprot
K334	Ubiquitination	Uniprot
S520	Phosphorylation	Uniprot
K528	Ubiquitination	Uniprot
K570	Ubiquitination	Uniprot
Y575	Phosphorylation	Uniprot
K596	Ubiquitination	Uniprot
K621	Ubiquitination	Uniprot
K643	Ubiquitination	Uniprot

Research Backgrounds

Q92611_EDEM1_HUMAN

Function:

Extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation (ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-independent manner, probably by forming a complex with SEL1L. It has low mannosidase activity, catalyzing mannose trimming from Man8GlcNAc2 to Man7GlcNAc2.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass type II membrane protein.

Subunit Structure:

Interacts with DNAJC10 (By similarity). Interacts with DERL2 and DERL3. Binds to SEL1L.

Family&Domains:

Belongs to the glycosyl hydrolase 47 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)

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EDEM1 Antibody - #DF9502