EDC4 Antibody - #DF9501

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Product: EDC4 Antibody
Catalog: DF9501
Description: Rabbit polyclonal antibody to EDC4
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 152 kDa; 152kD(Calculated).
Uniprot: Q6P2E9
RRID: AB_2842697

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
EDC4 Antibody detects endogenous levels of total EDC4.
RRID:
AB_2842697
Cite Format: Affinity Biosciences Cat# DF9501, RRID:AB_2842697.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

autoantigen; Autoantigen Ge 1; Autoantigen Ge-1; Autoantigen Ge1; Autoantigen RCD 8; Autoantigen RCD-8; Autoantigen RCD8; edc4; EDC4_HUMAN; Enhancer of mRNA decapping 4; Enhancer of mRNA-decapping protein 4; Ge 1; Hedls; Human enhancer of decapping large subunit; RCD 8;

Immunogens

Immunogen:
Uniprot:

Q6P2E9(EDC4_HUMAN) >>Visit HPA database.

Gene(ID):
EDC4(23644)
Sequence:
MASCASIDIEDATQHLRDILKLDRPAGGPSAESPRPSSAYNGDLNGLLVPDPLCSGDSTSANKTGLRTMPPINLQEKQVICLSGDDSSTCIGILAKEVEIVASSDSSISSKARGSNKVKIQPVAKYDWEQKYYYGNLIAVSNSFLAYAIRAANNGSAMVRVISVSTSERTLLKGFTGSVADLAFAHLNSPQLACLDEAGNLFVWRLALVNGKIQEEILVHIRQPEGTPLNHFRRIIWCPFIPEESEDCCEESSPTVALLHEDRAEVWDLDMLRSSHSTWPVDVSQIKQGFIVVKGHSTCLSEGALSPDGTVLATASHDGYVKFWQIYIEGQDEPRCLHEWKPHDGRPLSCLLFCDNHKKQDPDVPFWRFLITGADQNRELKMWCTVSWTCLQTIRFSPDIFSSVSVPPSLKVCLDLSAEYLILSDVQRKVLYVMELLQNQEEGHACFSSISEFLLTHPVLSFGIQVVSRCRLRHTEVLPAEEENDSLGADGTHGAGAMESAAGVLIKLFCVHTKALQDVQIRFQPQLNPDVVAPLPTHTAHEDFTFGESRPELGSEGLGSAAHGSQPDLRRIVELPAPADFLSLSSETKPKLMTPDAFMTPSASLQQITASPSSSSSGSSSSSSSSSSSLTAVSAMSSTSAVDPSLTRPPEELTLSPKLQLDGSLTMSSSGSLQASPRGLLPGLLPAPADKLTPKGPGQVPTATSALSLELQEVEPLGLPQASPSRTRSPDVISSASTALSQDIPEIASEALSRGFGSSAPEGLEPDSMASAASALHLLSPRPRPGPELGPQLGLDGGPGDGDRHNTPSLLEAALTQEASTPDSQVWPTAPDITRETCSTLAESPRNGLQEKHKSLAFHRPPYHLLQQRDSQDASAEQSDHDDEVASLASASGGFGTKVPAPRLPAKDWKTKGSPRTSPKLKRKSKKDDGDAAMGSRLTEHQVAEPPEDWPALIWQQQRELAELRHSQEELLQRLCTQLEGLQSTVTGHVERALETRHEQEQRRLERALAEGQQRGGQLQEQLTQQLSQALSSAVAGRLERSIRDEIKKTVPPCVSRSLEPMAGQLSNSVATKLTAVEGSMKENISKLLKSKNLTDAIARAAADTLQGPMQAAYREAFQSVVLPAFEKSCQAMFQQINDSFRLGTQEYLQQLESHMKSRKAREQEAREPVLAQLRGLVSTLQSATEQMAATVAGSVRAEVQHQLHVAVGSLQESILAQVQRIVKGEVSVALKEQQAAVTSSIMQAMRSAAGTPVPSAHLDCQAQQAHILQLLQQGHLNQAFQQALTAADLNLVLYVCETVDPAQVFGQPPCPLSQPVLLSLIQQLASDLGTRTDLKLSYLEEAVMHLDHSDPITRDHMGSVMAQVRQKLFQFLQAEPHNSLGKAARRLSLMLHGLVTPSLP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
82
Chicken
73
Xenopus
55
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q6P2E9 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S3 Phosphorylation
S6 Phosphorylation
K21 Ubiquitination
S30 Phosphorylation
S33 Phosphorylation
S37 Phosphorylation
K63 Ubiquitination
S104 Phosphorylation
S106 Phosphorylation
S107 Phosphorylation
S110 Phosphorylation
K111 Ubiquitination
K119 Ubiquitination
K125 Acetylation
K125 Ubiquitination
K287 Ubiquitination
K359 Ubiquitination
S486 Phosphorylation
S555 Phosphorylation
S560 Phosphorylation
S565 Phosphorylation
S583 Phosphorylation
S585 Phosphorylation
S586 Phosphorylation
T588 Phosphorylation
S613 Phosphorylation
S621 Phosphorylation
S622 Phosphorylation
S623 Phosphorylation
S624 Phosphorylation
S664 Phosphorylation
S676 Phosphorylation
T693 Phosphorylation
S708 Phosphorylation
S723 Phosphorylation
S725 Phosphorylation
T727 Phosphorylation
S729 Phosphorylation
S734 Phosphorylation
S735 Phosphorylation
S737 Phosphorylation
T738 Phosphorylation
S741 Phosphorylation
S780 Phosphorylation
T807 Phosphorylation
S809 Phosphorylation
S820 Phosphorylation
T821 Phosphorylation
S824 Phosphorylation
T837 Phosphorylation
S844 Phosphorylation
S855 Phosphorylation
Y863 Phosphorylation
S871 Phosphorylation
S875 Phosphorylation
S879 Phosphorylation
S887 Phosphorylation
S892 Phosphorylation
T911 Phosphorylation
S914 Phosphorylation
S918 Phosphorylation
R965 Methylation
S967 Phosphorylation
R1015 Methylation
T1050 Phosphorylation
C1054 S-Nitrosylation
S1058 Phosphorylation
S1067 Phosphorylation
T1072 Phosphorylation
T1075 Phosphorylation
S1080 Phosphorylation
K1082 Ubiquitination
K1087 Ubiquitination
K1092 Ubiquitination
Y1148 Phosphorylation
S1154 Phosphorylation
K1157 Ubiquitination
K1368 Ubiquitination
S1380 Phosphorylation
K1383 Ubiquitination
S1389 Phosphorylation
S1399 Phosphorylation

Research Backgrounds

Function:

In the process of mRNA degradation, seems to play a role in mRNA decapping. Component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. Promotes complex formation between DCP1A and DCP2. Enhances the catalytic activity of DCP2 (in vitro).

Subcellular Location:

Cytoplasm>P-body. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Part of a decapping complex consisting of DCP1A, DCP2, EDC3, EDC4 and probably DDX6. Part of a complex consisting of DCP1A, EDC3, EDC4 and DDX6. Part of a complex consisting of DCP1B, EDC3, EDC4 and DDX6. Interacts with DCP2. Interacts with RC3H1 (By similarity). Interacts with NBDY. Interacts with TEX19 (By similarity).

Family&Domains:

Belongs to the WD repeat EDC4 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.

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