NTHL1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from COS-7, using NTHL1 Antibody.

Product:	NTHL1 Antibody
Catalog:	DF9498
Description:	Rabbit polyclonal antibody to NTHL1
Application:	WB
Reactivity:	Human, Mouse, Monkey
Prediction:	Pig, Zebrafish, Bovine, Horse, Chicken, Xenopus
Mol.Wt.:	34 kDa; 34kD(Calculated).
Uniprot:	P78549
RRID:	AB_2842694

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Monkey

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

NTHL1 Antibody detects endogenous levels of total NTHL1.

RRID:

AB_2842694
Cite Format: Affinity Biosciences Cat# DF9498, RRID:AB_2842694.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Bifunctional DNA N glycoslyase/DNA (apurinic or apyrimidinic site) lyase; DNA glycoslyase/AP lyase; Endonuclease III like protein 1; Endonuclease III-like protein 1; hNTH1; NTH 1; nth endonuclease III like 1 (E. coli); NTH endonuclease III Like 1; NTH1; NTHL 1; Nthl1; NTHL1_HUMAN; OCTS 3; OCTS3;

Immunogens

Immunogen:

Uniprot:

P78549(NTH_HUMAN) >>Visit HPA database.

Gene(ID):

NTHL1(4913)

Expression:

P78549 NTH_HUMAN:

Widely expressed with highest levels in heart and lowest levels in lung and liver.

Sequence:

P78549 NTH_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MCSPQESGMTALSARMLTRSRSLGPGAGPRGCREEPGPLRRREAAAEARKSHSPVKRPRKAQRLRVAYEGSDSEKGEGAEPLKVPVWEPQDWQQQLVNIRAMRNKKDAPVDHLGTEHCYDSSAPPKVRRYQVLLSLMLSSQTKDQVTAGAMQRLRARGLTVDSILQTDDATLGKLIYPVGFWRSKVKYIKQTSAILQQHYGGDIPASVAELVALPGVGPKMAHLAMAVAWGTVSGIAVDTHVHRIANRLRWTKKATKSPEETRAALEEWLPRELWHEINGLLVGFGQQTCLPVHPRCHACLNQALCPAAQGL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Xenopus

100

Zebrafish

100

Chicken

100

Sheep

Dog

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P78549 As Substrate

P78549

Site	PTM Type	Source
S3	Phosphorylation	Uniprot
S20	Phosphorylation	Uniprot
R21	Methylation	Uniprot
S22	Phosphorylation	Uniprot
S53	Phosphorylation	Uniprot
Y68	Phosphorylation	Uniprot
S71	Phosphorylation	Uniprot
S73	Phosphorylation	Uniprot
K83	Ubiquitination	Uniprot
K106	Ubiquitination	Uniprot
S135	Phosphorylation	Uniprot
S140	Phosphorylation	Uniprot
T147	Phosphorylation	Uniprot
T160	Phosphorylation	Uniprot
S163	Phosphorylation	Uniprot
T171	Phosphorylation	Uniprot
K174	Ubiquitination	Uniprot
Y177	Phosphorylation	Uniprot

Research Backgrounds

P78549_NTH_HUMAN

Function:

Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines. Has also 8-oxo-7,8-dihydroguanine (8-oxoG) DNA glycosylase activity. Acts preferentially on DNA damage opposite guanine residues in DNA. Is able to process lesions in nucleosomes without requiring or inducing nucleosome disruption.

Subcellular Location:

Nucleus. Mitochondrion.

Tissue Specificity:

Widely expressed with highest levels in heart and lowest levels in lung and liver.

Subunit Structure:

Interacts with YBX1.

Family&Domains:

Belongs to the Nth/MutY family.

Research Fields

· Genetic Information Processing > Replication and repair > Base excision repair.

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NTHL1 Antibody - #DF9498