EEF1A2 Antibody | Affinity Biosciences

IF/ICC
Cites

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DF9493 staining Hela cells by IF/ICC.

Fig.

Supplementary Figure 3.

Fig.

Product:	EEF1A2 Antibody
Catalog:	DF9493
Description:	Rabbit polyclonal antibody to EEF1A2
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.:	50 kDa; 50kD(Calculated).
Uniprot:	Q05639
RRID:	AB_2842689

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(91%)

Clonality:

Polyclonal

Specificity:

EEF1A2 Antibody detects endogenous levels of total EEF1A2.

RRID:

AB_2842689
Cite Format: Affinity Biosciences Cat# DF9493, RRID:AB_2842689.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CCS3; eEF1A-1; eEF1A-2; EEF1A1; Eef1a2; EEF1AL; EF 1 alpha 2; EF-1-alpha-1; EF-1-alpha-2; EF-Tu; EF1A; EF1A1_HUMAN; EF1A2_HUMAN; Elongation factor 1 A 2; Elongation factor 1 alpha; Elongation factor 1 alpha 2; Elongation factor 1-alpha 1; Elongation factor 1-alpha 2; Elongation factor Tu; Eukaryotic elongation factor 1 A-1; Eukaryotic elongation factor 1 A-2; Eukaryotic translation elongation factor 1 alpha 2; GRAF 1EF; HS1; Leukocyte receptor cluster member 7; PTI1; Statin-S1;

Immunogens

Immunogen:

Uniprot:

Q05639(EF1A2_HUMAN) >>Visit HPA database.

Gene(ID):

EEF1A2(1917)

Expression:

Q05639 EF1A2_HUMAN:

Brain, heart, and skeletal muscle.

Sequence:

Q05639 EF1A2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETTKYYITIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPAYSEKRYDEIVKEVSAYIKKIGYNPATVPFVPISGWHGDNMLEPSPNMPWFKGWKVERKEGNASGVSLLEALDTILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGILRPGMVVTFAPVNITTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDIRRGNVCGDSKSDPPQEAAQFTSQVIILNHPGQISAGYSPVIDCHTAHIACKFAELKEKIDRRSGKKLEDNPKSLKSGDAAIVEMVPGKPMCVESFSQYPPLGRFAVRDMRQTVAVGVIKNVEKKSGGAGKVTKSAQKAQKAGK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Chicken

100

Rabbit

100

Xenopus

Zebrafish

Dog

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q05639 As Substrate

Q05639

Site	PTM Type	Enzyme	Source
S21	Phosphorylation	P15056 (BRAF) , P04049 (RAF1)	Uniprot
T22	Phosphorylation		Uniprot
T23	Phosphorylation		Uniprot
T24	Phosphorylation		Uniprot
Y29	Phosphorylation		Uniprot
K30	Acetylation		Uniprot
K30	Methylation		Uniprot
K30	Ubiquitination		Uniprot
K36	Acetylation		Uniprot
K36	Methylation		Uniprot
K36	Ubiquitination		Uniprot
R37	Methylation		Uniprot
K41	Acetylation		Uniprot
K41	Sumoylation		Uniprot
K41	Ubiquitination		Uniprot
K44	Acetylation		Uniprot
K44	Ubiquitination		Uniprot
K51	Methylation		Uniprot
K51	Ubiquitination		Uniprot
S53	Phosphorylation	P05771 (PRKCB)	Uniprot
K55	Acetylation		Uniprot
K55	Methylation		Uniprot
K55	Sumoylation		Uniprot
K55	Ubiquitination		Uniprot
Y56	Phosphorylation		Uniprot
K62	Methylation		Uniprot
K62	Ubiquitination		Uniprot
R69	Methylation		Uniprot
K79	Acetylation		Uniprot
K79	Methylation		Uniprot
K84	Methylation		Uniprot
Y85	Phosphorylation		Uniprot
Y86	Phosphorylation		Uniprot
T88	Phosphorylation		Uniprot
T104	Phosphorylation		Uniprot
T106	Phosphorylation		Uniprot
S128	Phosphorylation		Uniprot
K129	Ubiquitination		Uniprot
T133	Phosphorylation		Uniprot
R134	Methylation		Uniprot
Y141	Phosphorylation		Uniprot
T142	Phosphorylation		Uniprot
K146	Acetylation		Uniprot
K146	Ubiquitination		Uniprot
K154	Ubiquitination		Uniprot
S163	Phosphorylation		Uniprot
K165	Acetylation		Uniprot
K165	Methylation		Uniprot
K165	Ubiquitination		Uniprot
R166	Methylation		Uniprot
K172	Ubiquitination		Uniprot
S175	Phosphorylation		Uniprot
Y177	Phosphorylation		Uniprot
K179	Acetylation		Uniprot
S205	Phosphorylation	P45983 (MAPK8)	Uniprot
K219	Ubiquitination		Uniprot
T234	Phosphorylation		Uniprot
T239	Phosphorylation		Uniprot
K244	Ubiquitination		Uniprot
Y254	Phosphorylation		Uniprot
K255	Acetylation		Uniprot
K255	Methylation		Uniprot
K255	Ubiquitination		Uniprot
T261	Phosphorylation		Uniprot
R266	Methylation		Uniprot
T269	Phosphorylation		Uniprot
T287	Phosphorylation		Uniprot
S291	Phosphorylation		Uniprot
S300	Phosphorylation		Uniprot
K313	Sumoylation		Uniprot
K313	Ubiquitination		Uniprot
K318	Acetylation		Uniprot
K318	Methylation		Uniprot
K318	Ubiquitination		Uniprot
S358	Phosphorylation	P45983 (MAPK8)	Uniprot
S383	Phosphorylation		Uniprot
K392	Ubiquitination		Uniprot
S393	Phosphorylation		Uniprot
R430	Methylation		Uniprot
T432	Phosphorylation		Uniprot
K439	Acetylation		Uniprot
S445	Phosphorylation		Uniprot
T452	Phosphorylation		Uniprot
K453	Ubiquitination		Uniprot

Research Backgrounds

Q05639_EF1A2_HUMAN

Function:

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

PTMs:

Trimethylated at Lys-165 by EEF1AKMT3. Mono-, di-, and trimethylated at Lys-36 by EEF1AKMT4; trimethylated form is predominant. Methylation by EEF1AKMT4 contributes to the fine-tuning of translation rates for a subset of tRNAs. Trimethylated at the N-terminus by EEF1AKNMT. Mono- and dimethylated at Lys-55 by EEF1AKNMT; dimethylated form is predominant.

Subcellular Location:

Nucleus.

Tissue Specificity:

Brain, heart, and skeletal muscle.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

References

1). EEF1A2 interacts with HSP90AB1 to promote lung adenocarcinoma metastasis via enhancing TGF-β/SMAD signalling. BRITISH JOURNAL OF CANCER, 2021 (PubMed: 33473168) [IF=8.8]

Application: WB Species: human Sample: A549 and PC9 cells

Fig. 6| EEF1A2 regulates TGF-β/SMAD signalling based on HSP90AB1. a HSP90AB1 silencing inhibited the invasion potential of A549 cells induced by EEF1A2 overexpression. Data are shown as mean ± SEM; n = 3 independent experiments, two-tailed Student’s t-test. b A549 and PC9 cells were co-transfected with PCDH-EEF1A2, and si-HSP90AB1. The protein expression levels of HSP90AB1, EEF1A1, TβRI, TβRII, SMAD3,pSMAD3, N-cadherin and SLUG were detected using western blot analysis.

Application: IF/ICC Species: human Sample: A549 cells

Supplementary Figure 3.| EEF1A2 interacts with HSP90AB1. (D) Confocal images shown position of co-location EEF1A2 (green) and HSP90AB1 (red) after transfection of si-EEF1A2 or si-HSP90AB1 in A549 cells. Images were acquired at 400×. Scale bar = 50 µm.

2). Biliverdin modulates the Nrf2/A20/eEF1A2 axis to alleviate cerebral ischemia-reperfusion injury by inhibiting pyroptosis. Biomedicine & Pharmacotherapy, 2023 (PubMed: 37399716) [IF=7.5]

Application: WB Species: Mouse Sample:

Fig. 8. The interaction of A20 with eEF1A2. A. Mass spectrum data of eEF1A2 among A20-interacting proteins identified by mass spectrometry. B. Co-IP assay confirmed the interaction between A20 and eEF1A2, with or without MG-132; C. eEF1A2 mRNA expression; D. eEF1A2 protein expression; E. Western blot images of eEF1A2 and GAPDH.

3). Cyclin-dependent kinase subunit2 (CKS2) promotes malignant phenotypes and epithelial-mesenchymal transition-like process in glioma by activating TGFβ/SMAD signaling. Cancer Medicine, 2023 (PubMed: 36284444) [IF=4.0]

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EEF1A2 Antibody - #DF9493