MARCH6 Antibody - #DF9482

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Product: MARCH6 Antibody
Catalog: DF9482
Description: Rabbit polyclonal antibody to MARCH6
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Rabbit, Chicken, Xenopus
Mol.Wt.: 103 kDa; 103kD(Calculated).
Uniprot: O60337
RRID: AB_2842678

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(90%), Rabbit(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
MARCH6 Antibody detects endogenous levels of total MARCH6.
RRID:
AB_2842678
Cite Format: Affinity Biosciences Cat# DF9482, RRID:AB_2842678.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DOA10; Doa10 homolog; E3 ubiquitin protein ligase MARCH6; KIAA0597; MARCH 6; MARCH VI; MARCHVI; Membrane associated RING CH protein VI; Membrane associated ring finger (C3HC4) 6; Membrane associated RING finger protein 6; Protein TEB 4; Protein TEB4; RING finger protein 176; RNF 176; RNF176; TEB 4; TEB4;

Immunogens

Immunogen:
Uniprot:

O60337(MARH6_HUMAN) >>Visit HPA database.

Gene(ID):
MARCH6(10299)
Expression:
O60337 MARH6_HUMAN:

Present in brain (at protein level).

Sequence:
MDTAEEDICRVCRSEGTPEKPLYHPCVCTGSIKFIHQECLVQWLKHSRKEYCELCKHRFAFTPIYSPDMPSRLPIQDIFAGLVTSIGTAIRYWFHYTLVAFAWLGVVPLTACRIYKCLFTGSVSSLLTLPLDMLSTENLLADCLQGCFVVTCTLCAFISLVWLREQIVHGGAPIWLEHAAPPFNAAGHHQNEAPAGGNGAENVAADQPANPPAENAVVGENPDAQDDQAEEEEEDNEEEDDAGVEDAADANNGAQDDMNWNALEWDRAAEELTWERMLGLDGSLVFLEHVFWVVSLNTLFILVFAFCPYHIGHFSLVGLGFEEHVQASHFEGLITTIVGYILLAITLIICHGLATLVKFHRSRRLLGVCYIVVKVSLLVVVEIGVFPLICGWWLDICSLEMFDATLKDRELSFQSAPGTTMFLHWLVGMVYVFYFASFILLLREVLRPGVLWFLRNLNDPDFNPVQEMIHLPIYRHLRRFILSVIVFGSIVLLMLWLPIRIIKSVLPNFLPYNVMLYSDAPVSELSLELLLLQVVLPALLEQGHTRQWLKGLVRAWTVTAGYLLDLHSYLLGDQEENENSANQQVNNNQHARNNNAIPVVGEGLHAAHQAILQQGGPVGFQPYRRPLNFPLRIFLLIVFMCITLLIASLICLTLPVFAGRWLMSFWTGTAKIHELYTAACGLYVCWLTIRAVTVMVAWMPQGRRVIFQKVKEWSLMIMKTLIVAVLLAGVVPLLLGLLFELVIVAPLRVPLDQTPLFYPWQDWALGVLHAKIIAAITLMGPQWWLKTVIEQVYANGIRNIDLHYIVRKLAAPVISVLLLSLCVPYVIASGVVPLLGVTAEMQNLVHRRIYPFLLMVVVLMAILSFQVRQFKRLYEHIKNDKYLVGQRLVNYERKSGKQGSSPPPPQSSQE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
90
Horse
0
Bovine
0
Sheep
0
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60337 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S14 Phosphorylation
T17 Phosphorylation
K20 Ubiquitination
T29 Phosphorylation
S31 Phosphorylation
K45 Ubiquitination
K49 Ubiquitination
K56 Ubiquitination
S437 Phosphorylation
Y474 Phosphorylation
K550 Ubiquitination
Y850 Phosphorylation
Y874 Phosphorylation
K878 Ubiquitination
K881 Ubiquitination
Y882 Phosphorylation
K897 Ubiquitination

Research Backgrounds

Function:

E3 ubiquitin-protein ligase that promotes 'Lys-48'-linked ubiquitination of target proteins, leading to their proteasomal degradation. Promotes ubiquitination of DIO2, leading to its degradation. Promotes ubiquitination of SQLE, leading to its degradation. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. May cooperate with UBE2G1.

PTMs:

Auto-ubiquitinated, which results in proteasomal degradation.

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Present in brain (at protein level).

Subunit Structure:

Interacts with DIO2. Interacts with SQLE.

Family&Domains:

The RING-CH-type zinc finger domain is required for E3 ligase activity.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

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