RPA49 Antibody - #DF9457
| Product: | RPA49 Antibody |
| Catalog: | DF9457 |
| Description: | Rabbit polyclonal antibody to RPA49 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 54 kDa; 47kD(Calculated). |
| Uniprot: | Q9GZS1 |
| RRID: | AB_2842653 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF9457, RRID:AB_2842653.
Fold/Unfold
A49; DNA directed RNA polymerase I subunit RPA49; DNA-directed RNA polymerase I subunit E; DNA-directed RNA polymerase I subunit RPA49; FLJ 13390; FLJ13970; PAF53; POLR 1 E; POLR1E; Polymerase (RNA) I associated factor 1; Polymerase (RNA) I polypeptide E; Polymerase (RNA) I polypeptide E, 53kDa; PRAF1; RNA polymerase I associated factor 1; RNA polymerase I associated factor 53; RNA polymerase I polypeptide E; RNA polymerase I subunit A49; RNA polymerase I-associated factor 1; RNA polymerase I-associated factor 53; RP11405L18.3; RPA49_HUMAN;
Immunogens
- Q9GZS1 RPA49_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAEVLPSARWQYCGAPDGSQRAVLVQFSNGKLQSPGNMRFTLYENKDSTNPRKRNQRILAAETDRLSYVGNNFGTGALKCNTLCRHFVGILNKTSGQMEVYDAELFNMQPLFSDVSVESELALESQTKTYREKMDSCIEAFGTTKQKRALNTRRMNRVGNESLNRAVAKAAETIIDTKGVTALVSDAIHNDLQDDSLYLPPCYDDAAKPEDVYKFEDLLSPAEYEALQSPSEAFRNVTSEEILKMIEENSHCTFVIEALKSLPSDVESRDRQARCIWFLDTLIKFRAHRVVKRKSALGPGVPHIINTKLLKHFTCLTYNNGRLRNLISDSMKAKITAYVIILALHIHDFQIDLTVLQRDLKLSEKRMMEIAKAMRLKISKRRVSVAAGSEEDHKLGTLSLPLPPAQTSDRLAKRRKIT
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9GZS1 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S8 | Phosphorylation | Uniprot | |
| S35 | Phosphorylation | Uniprot | |
| R40 | Methylation | Uniprot | |
| K47 | Ubiquitination | Uniprot | |
| S49 | Phosphorylation | Uniprot | |
| T64 | Phosphorylation | Uniprot | |
| R66 | Methylation | Uniprot | |
| K80 | Methylation | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| Y131 | Phosphorylation | Uniprot | |
| K134 | Ubiquitination | Uniprot | |
| T144 | Phosphorylation | Uniprot | |
| T145 | Phosphorylation | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| S163 | Phosphorylation | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| K179 | Sumoylation | Uniprot | |
| K179 | Ubiquitination | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| S265 | Phosphorylation | Uniprot | |
| K295 | Methylation | Uniprot | |
| S296 | Phosphorylation | Uniprot | |
| K309 | Ubiquitination | Uniprot | |
| K366 | Acetylation | Uniprot | |
| K366 | Ubiquitination | Uniprot | |
| K373 | Acetylation | Uniprot | |
| S385 | Phosphorylation | Uniprot | |
| S390 | Phosphorylation | Uniprot |
Research Backgrounds
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF.
Acetylated at Lys-373 by CREBBP/CBP, leading to decreased RNA polymerase I transcription. In normal conditions, deacetylated by SIRT7, promoting the association of RNA polymerase I with the rDNA promoter region and coding region. In response to stress, SIRT7 is released from nucleoli leading to hyperacetylation of POLR1E/PAF53 and decreased association of RNA polymerase I with the rDNA promoter region.
Nucleus>Nucleolus.
Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits. Interacts with PAF49/CD3EAP (By similarity). Also binds UBTF/UBF (By similarity). Interacts with PWP1.
Belongs to the eukaryotic RPA49/POLR1E RNA polymerase subunit family.
Research Fields
· Genetic Information Processing > Transcription > RNA polymerase.
· Metabolism > Nucleotide metabolism > Purine metabolism.
· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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