DGAT2 Antibody | Affinity Biosciences

WB
IF/ICC
Cites

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Western blot analysis of extracts from Mouse testis, using DGAT2 Antibody.

Figure 6 Down-regulation of BMP5 attenuates mitochondrial fat oxidation and triglyceride synthesis.

Product:	DGAT2 Antibody
Catalog:	DF9442
Description:	Rabbit polyclonal antibody to DGAT2
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	44 kDa, 55 kDa; 44kD(Calculated).
Uniprot:	Q96PD7
RRID:	AB_2842638

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%), Xenopus(92%)

Clonality:

Polyclonal

Specificity:

DGAT2 Antibody detects endogenous levels of total DGAT2.

RRID:

AB_2842638
Cite Format: Affinity Biosciences Cat# DF9442, RRID:AB_2842638.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DGAT2; DGAT2_HUMAN; Diacylglycerol O acyltransferase like protein 2; Diacylglycerol O-acyltransferase 2; Diacylglycerol O-acyltransferase homolog 2 (mouse); Diacylglycerol O-acyltransferase homolog 2; Diacylglycerol O-acyltransferase-like protein 2 isoform 1; Diglyceride acyltransferase 2; DKFZp686A15125; GS1999full; HMFN1045;

Immunogens

Immunogen:

Uniprot:

Q96PD7(DGAT2_HUMAN) >>Visit HPA database.

Gene(ID):

DGAT2(84649)

Expression:

Q96PD7 DGAT2_HUMAN:

Predominantly expressed in liver and white adipose tissue. Expressed at lower level in mammary gland, testis and peripheral blood leukocytes. Expressed in sebaceous glands of normal skin but decreased psoriatic skin.

Sequence:

Q96PD7 DGAT2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKTLIAAYSGVLRGERQAEADRSQRSHGGPALSREGSGRWGTGSSILSALQDLFSVTWLNRSKVEKQLQVISVLQWVLSFLVLGVACSAILMYIFCTDCWLIAVLYFTWLVFDWNTPKKGGRRSQWVRNWAVWRYFRDYFPIQLVKTHNLLTTRNYIFGYHPHGIMGLGAFCNFSTEATEVSKKFPGIRPYLATLAGNFRMPVLREYLMSGGICPVSRDTIDYLLSKNGSGNAIIIVVGGAAESLSSMPGKNAVTLRNRKGFVKLALRHGADLVPIYSFGENEVYKQVIFEEGSWGRWVQKKFQKYIGFAPCIFHGRGLFSSDTWGLVPYSKPITTVVGEPITIPKLEHPTQQDIDLYHTMYMEALVKLFDKHKTKFGLPETEVLEVN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96PD7 As Substrate

Q96PD7

Site	PTM Type	Source
S23	Phosphorylation	Uniprot
S26	Phosphorylation	Uniprot
S33	Phosphorylation	Uniprot
S37	Phosphorylation	Uniprot
K146	Ubiquitination	Uniprot
K260	Methylation	Uniprot
Y306	Phosphorylation	Uniprot
S331	Phosphorylation	Uniprot
K376	Ubiquitination	Uniprot

Research Backgrounds

Q96PD7_DGAT2_HUMAN

Function:

Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation. In liver, is primarily responsible for incorporating endogenously synthesized fatty acids into triglycerides (By similarity). Functions also as an acyl-CoA retinol acyltransferase (ARAT).

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein. Lipid droplet. Cytoplasm>Perinuclear region.

Tissue Specificity:

Subunit Structure:

Forms multimeric complexes consisting of several DGAT2 subunits.

Family&Domains:

Belongs to the diacylglycerol acyltransferase family.

Research Fields

· Metabolism > Lipid metabolism > Glycerolipid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Digestive system > Fat digestion and absorption.

References

1). Mitochondrial dysfunction resulting from the down-regulation of bone morphogenetic protein 5 may cause microtia. Annals of Translational Medicine, 2021 (PubMed: 33842639)

Application: WB Species: Human Sample: 293T cells

Figure 6 Down-regulation of BMP5 attenuates mitochondrial fat oxidation and triglyceride synthesis. (A) The expression of CPT2 mRNA in 293T cells treated with BMP5-siRNA shows a larger decrease than that in the siNC group. (B) The expression of CPT2 mRNA in ADSCs treated with BMP5-siRNA decreased. (C) CPT2 protein expression in 293T cells treated with BMP5-siRNA shows a larger decrease than that in the siNC group. (D) The expression of DGAT2 mRNA in 293T cells treated with BMP5-siRNA decreased. (E) The expression of DGAT2 mRNA in ADSCs treated with BMP5-siRNA decreased. (F) DGAT2 protein expression in 293T cells treated with BMP5-siRNA decreased (G) Representative western blots of CPT2 and DGAT2 enzymes in 3 groups of 293T cells. *, P<0.05; **, P<0.01. BMP5, bone morphogenetic protein 5; ADSC, adipose-derived stem cells; NC, normal control; CPT2, carnitine O-palmitoyltransferase 2; DGAT2, diacylglycerol acyltransferase 2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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DGAT2 Antibody - #DF9442