NT5C2 Antibody - #DF9431

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Product: NT5C2 Antibody
Catalog: DF9431
Description: Rabbit polyclonal antibody to NT5C2
Application: WB IHC
Reactivity: Human, Mouse, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 65 kDa; 65kD(Calculated).
Uniprot: P49902
RRID: AB_2842627

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Monkey
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
NT5C2 Antibody detects endogenous levels of total NT5C2.
RRID:
AB_2842627
Cite Format: Affinity Biosciences Cat# DF9431, RRID:AB_2842627.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

5' nucleotidase (purine) cytosolic type B; 5NTC_HUMAN; cN II; Cytosolic 5''-nucleotidase II; Cytosolic IMP/GMP-specific 5'-nucleotidase; Cytosolic purine 5' nucleotidase; Cytosolic purine 5''-nucleotidase; GMP; NT5B; nt5c2; NT5CP; NUCLEOTIDASE, 5-PRIME, CYTOSOLIC II; NUCLEOTIDASE, 5-PRIME, TYPE B; PNT5; PURINE 5-PRIME-NUCLEOTIDASE;

Immunogens

Immunogen:
Uniprot:

P49902(5NTC_HUMAN) >>Visit HPA database.

Gene(ID):
NT5C2(22978)
Sequence:
MSTSWSDRLQNAADMPANMDKHALKKYRREAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSIGYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFIRGPETREQYPNKFIQRDDTERFYILNTLFNLPETYLLACLVDFFTNCPRYTSCETGFKDGDLFMSYRSMFQDVRDAVDWVHYKGSLKEKTVENLEKYVVKDGKLPLLLSRMKEVGKVFLATNSDYKYTDKIMTYLFDFPHGPKPGSSHRPWQSYFDLILVDARKPLFFGEGTVLRQVDTKTGKLKIGTYTGPLQHGIVYSGGSSDTICDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDINEMESPLATRNRTSVDFKDTDYKRHQLTRSISEIKPPNLFPLAPQEITHCHDEDDDEEEEEEEE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49902 As Substrate

Site PTM Type Enzyme
T3 Phosphorylation
S4 Phosphorylation
K21 Ubiquitination
K26 Acetylation
Y32 Phosphorylation
K140 Ubiquitination
Y178 Phosphorylation
K186 Ubiquitination
S193 Phosphorylation
K211 Ubiquitination
K217 Ubiquitination
K224 Ubiquitination
K228 Ubiquitination
K231 Ubiquitination
S237 Phosphorylation
K244 Ubiquitination
K254 Ubiquitination
K292 Ubiquitination
K313 Ubiquitination
K342 Ubiquitination
K344 Ubiquitination
K359 Ubiquitination
S410 Phosphorylation
S418 Phosphorylation
T427 Phosphorylation
S439 Phosphorylation
S502 Phosphorylation
T510 Phosphorylation
S511 Phosphorylation
K515 Ubiquitination
Y519 Phosphorylation
K520 Ubiquitination
S527 Phosphorylation
S529 Phosphorylation
K532 Ubiquitination

Research Backgrounds

Function:

May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homotetramer.

Family&Domains:

Belongs to the 5'(3')-deoxyribonucleotidase family.

Research Fields

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Metabolism of cofactors and vitamins > Nicotinate and nicotinamide metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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