DYNC1I2 Antibody - #DF9427
| Product: | DYNC1I2 Antibody |
| Catalog: | DF9427 |
| Description: | Rabbit polyclonal antibody to DYNC1I2 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Horse, Sheep, Dog, Xenopus |
| Mol.Wt.: | 71 kDa; 71kD(Calculated). |
| Uniprot: | Q13409 |
| RRID: | AB_2842623 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF9427, RRID:AB_2842623.
Fold/Unfold
Cytoplasmic dynein 1 intermediate chain 2; Cytoplasmic dynein intermediate chain 2; cytosolic; DC1I2_HUMAN; DH IC 2; DH IC-2; DNCI2; DNCIC2; Dync1i2; Dynein intermediate chain 2; Dynein intermediate chain 2, cytosolic; Dynein, cytoplasmic, intermediate polypeptide 2; IC2;
Immunogens
- Q13409 DC1I2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSDKSELKAELERKKQRLAQIREEKKRKEEERKKKETDQKKEAVAPVQEESDLEKKRREAEALLQSMGLTPESPIVFSEYWVPPPMSPSSKSVSTPSEAGSQDSGDGAVGSRTLHWDTDPSVLQLHSDSDLGRGPIKLGMAKITQVDFPPREIVTYTKETQTPVMAQPKEDEEEDDDVVAPKPPIEPEEEKTLKKDEENDSKAPPHELTEEEKQQILHSEEFLSFFDHSTRIVERALSEQINIFFDYSGRDLEDKEGEIQAGAKLSLNRQFFDERWSKHRVVSCLDWSSQYPELLVASYNNNEDAPHEPDGVALVWNMKYKKTTPEYVFHCQSAVMSATFAKFHPNLVVGGTYSGQIVLWDNRSNKRTPVQRTPLSAAAHTHPVYCVNVVGTQNAHNLISISTDGKICSWSLDMLSHPQDSMELVHKQSKAVAVTSMSFPVGDVNNFVVGSEEGSVYTACRHGSKAGISEMFEGHQGPITGIHCHAAVGAVDFSHLFVTSSFDWTVKLWTTKNNKPLYSFEDNADYVYDVMWSPTHPALFACVDGMGRLDLWNLNNDTEVPTASISVEGNPALNRVRWTHSGREIAVGDSEGQIVIYDVGEQIAVPRNDEWARFGRTLAEINANRADAEEEAATRIPA
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13409 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| K8 | Acetylation | Uniprot | |
| K8 | Ubiquitination | Uniprot | |
| S51 | Phosphorylation | Uniprot | |
| S73 | Phosphorylation | Uniprot | |
| S87 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S89 | Phosphorylation | Uniprot | |
| S90 | Phosphorylation | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| T95 | Phosphorylation | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| S104 | Phosphorylation | Uniprot | |
| K137 | Ubiquitination | Uniprot | |
| T162 | Phosphorylation | Uniprot | |
| K182 | Ubiquitination | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| T209 | Phosphorylation | Uniprot | |
| S219 | Phosphorylation | Uniprot | |
| S238 | Phosphorylation | Uniprot | |
| K255 | Ubiquitination | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| R269 | Methylation | Uniprot | |
| K427 | Ubiquitination | Uniprot |
Research Backgrounds
Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. Involved in membrane-transport, such as Golgi apparatus, late endosomes and lysosomes. Has a critical role in neurodevelopment.
The phosphorylation status of Ser-90 appears to be involved in dynactin-dependent target binding.
Cytoplasm>Cytoskeleton. Cytoplasm.
Note: Detected in the cytoplasm of pachytene spermatocytes. Localizes to the manchette in elongating spermatids.
Homodimer (By similarity). The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. Interacts with DYNLT1 and DYNLT3. Interacts with DCNT1 (By similarity). Interacts with BICD2 (By similarity). Interacts with SPEF2 (By similarity).
(Microbial infection) Interacts with human adenovirus 5 hexon protein; this interaction probably allows virus intracellular transport.
Belongs to the dynein intermediate chain family.
Research Fields
· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.
· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.
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