CNGB3 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

Western blot analysis of extracts from Mouse spleen, using CNGB3 Antibody.

DF9420 at 1/100 staining rat brain tissue by IHC-P.

Product:	CNGB3 Antibody
Catalog:	DF9420
Description:	Rabbit polyclonal antibody to CNGB3
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Mol.Wt.:	92 kDa; 92kD(Calculated).
Uniprot:	Q9NQW8
RRID:	AB_2842616

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

CNGB3 Antibody detects endogenous levels of total CNGB3.

RRID:

AB_2842616
Cite Format: Affinity Biosciences Cat# DF9420, RRID:AB_2842616.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ACHM1; ACHM3; Achromatopsia (rod monochromacy) 3; CNG channel beta-3; CNGB3; CNGB3_HUMAN; Cone photoreceptor cGMP-gated cation channel beta-subunit; Cone photoreceptor cGMP-gated channel subunit beta; Cyclic nucleotide gated channel beta 3; Cyclic nucleotide-gated cation channel beta-3; Cyclic nucleotide-gated cation channel modulatory subunit; Cyclic nucleotide-gated channel beta-3; RMCH; RMCH1;

Immunogens

Immunogen:

Uniprot:

Q9NQW8(CNGB3_HUMAN) >>Visit HPA database.

Gene(ID):

CNGB3(54714)

Expression:

Q9NQW8 CNGB3_HUMAN:

Expressed specifically in the retina.

Sequence:

Q9NQW8 CNGB3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MFKSLTKVNKVKPIGENNENEQSSRRNEEGSHPSNQSQQTTAQEENKGEEKSLKTKSTPVTSEEPHTNIQDKLSKKNSSGDLTTNPDPQNAAEPTGTVPEQKEMDPGKEGPNSPQNKPPAAPVINEYADAQLHNLVKRMRQRTALYKKKLVEGDLSSPEASPQTAKPTAVPPVKESDDKPTEHYYRLLWFKVKKMPLTEYLKRIKLPNSIDSYTDRLYLLWLLLVTLAYNWNCCFIPLRLVFPYQTADNIHYWLIADIICDIIYLYDMLFIQPRLQFVRGGDIIVDSNELRKHYRTSTKFQLDVASIIPFDICYLFFGFNPMFRANRMLKYTSFFEFNHHLESIMDKAYIYRVIRTTGYLLFILHINACVYYWASNYEGIGTTRWVYDGEGNEYLRCYYWAVRTLITIGGLPEPQTLFEIVFQLLNFFSGVFVFSSLIGQMRDVIGAATANQNYFRACMDDTIAYMNNYSIPKLVQKRVRTWYEYTWDSQRMLDESDLLKTLPTTVQLALAIDVNFSIISKVDLFKGCDTQMIYDMLLRLKSVLYLPGDFVCKKGEIGKEMYIIKHGEVQVLGGPDGTKVLVTLKAGSVFGEISLLAAGGGNRRTANVVAHGFANLLTLDKKTLQEILVHYPDSERILMKKARVLLKQKAKTAEATPPRKDLALLFPPKEETPKLFKTLLGGTGKASLARLLKLKREQAAQKKENSEGGEEEGKENEDKQKENEDKQKENEDKGKENEDKDKGREPEEKPLDRPECTASPIAVEEEPHSVRRTVLPRGTSRQSLIISMAPSAEGGEEVLTIEVKEKAKQ

PTMs - Q9NQW8 As Substrate

Q9NQW8

Site	PTM Type	Source
S31	Phosphorylation	Uniprot
T40	Phosphorylation	Uniprot
T41	Phosphorylation	Uniprot
K51	Ubiquitination	Uniprot
S74	Phosphorylation	Uniprot
T95	Phosphorylation	Uniprot
T97	Phosphorylation	Uniprot
K166	Acetylation	Uniprot
Y200	Phosphorylation	Uniprot
Y545	Phosphorylation	Uniprot
T652	Phosphorylation	Uniprot
T656	Phosphorylation	Uniprot
T678	Phosphorylation	Uniprot
S687	Phosphorylation	Uniprot
K714	Acetylation	Uniprot

Research Backgrounds

Q9NQW8_CNGB3_HUMAN

Function:

Visual signal transduction is mediated by a G-protein coupled cascade using cGMP as second messenger. This protein can be activated by cGMP which leads to an opening of the cation channel and thereby causing a depolarization of rod photoreceptors. Induced a flickering channel gating, weakened the outward rectification in the presence of extracellular calcium, increased sensitivity for L-cis diltiazem and enhanced the cAMP efficiency of the channel when coexpressed with CNGA3 (By similarity). Essential for the generation of light-evoked electrical responses in the red-, green- and blue sensitive cones.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Tissue Specificity:

Expressed specifically in the retina.

Subunit Structure:

Tetramer formed of three CNGA3 and one CNGB3 modulatory subunits.

Family&Domains:

Belongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family. CNGB3 subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

Restrictive clause

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For Research Use Only.
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CNGB3 Antibody - #DF9420