CNGA3 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of CNGA3 using COLO205 whole cell lysates.

Product:	CNGA3 Antibody
Catalog:	DF9419
Description:	Rabbit polyclonal antibody to CNGA3
Application:	WB IF/ICC
Reactivity:	Human
Mol.Wt.:	79 kDa; 79kD(Calculated).
Uniprot:	Q16281
RRID:	AB_2842615

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

CNGA3 Antibody detects endogenous levels of total CNGA3.

RRID:

AB_2842615
Cite Format: Affinity Biosciences Cat# DF9419, RRID:AB_2842615.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ACHM2; CCNC1; CCNCa; CCNCalpha; CNCG3; CNG 3; CNG channel alpha 3; CNG channel alpha-3; CNG-3; CNG3; CNGA3; Cnga3 cyclic nucleotide gated channel alpha 3; CNGA3_HUMAN; Cone photoreceptor cGMP gated channel; Cone photoreceptor cGMP gated channel alpha subunit; Cone photoreceptor cGMP gated channel subunit alpha; Cone photoreceptor cGMP-gated channel subunit alpha; Cyclic nucleotide gated cation channel alpha 3; Cyclic nucleotide gated channel alpha 3; Cyclic nucleotide gated channel olfactory 3; Cyclic nucleotide gated channel rod photoreceptor subunit alpha; Cyclic nucleotide-gated cation channel alpha-3; Cyclic nucleotide-gated channel alpha-3; OTTHUMP00000161120;

Immunogens

Immunogen:

Uniprot:

Q16281(CNGA3_HUMAN) >>Visit HPA database.

Gene(ID):

CNGA3(1261)

Expression:

Q16281 CNGA3_HUMAN:

Prominently expressed in retina.

Sequence:

Q16281 CNGA3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAKINTQYSHPSRTHLKVKTSDRDLNRAENGLSRAHSSSEETSSVLQPGIAMETRGLADSGQGSFTGQGIARLSRLIFLLRRWAARHVHHQDQGPDSFPDRFRGAELKEVSSQESNAQANVGSQEPADRGRSAWPLAKCNTNTSNNTEEEKKTKKKDAIVVDPSSNLYYRWLTAIALPVFYNWYLLICRACFDELQSEYLMLWLVLDYSADVLYVLDVLVRARTGFLEQGLMVSDTNRLWQHYKTTTQFKLDVLSLVPTDLAYLKVGTNYPEVRFNRLLKFSRLFEFFDRTETRTNYPNMFRIGNLVLYILIIIHWNACIYFAISKFIGFGTDSWVYPNISIPEHGRLSRKYIYSLYWSTLTLTTIGETPPPVKDEEYLFVVVDFLVGVLIFATIVGNVGSMISNMNASRAEFQAKIDSIKQYMQFRKVTKDLETRVIRWFDYLWANKKTVDEKEVLKSLPDKLKAEIAINVHLDTLKKVRIFQDCEAGLLVELVLKLRPTVFSPGDYICKKGDIGKEMYIINEGKLAVVADDGVTQFVVLSDGSYFGEISILNIKGSKSGNRRTANIRSIGYSDLFCLSKDDLMEALTEYPEAKKALEEKGRQILMKDNLIDEELARAGADPKDLEEKVEQLGSSLDTLQTRFARLLAEYNATQMKMKQRLSQLESQVKGGGDKPLADGEVPGDATKTEDKQQ

PTMs - Q16281 As Substrate

Q16281

Site	PTM Type	Source
K138	Acetylation	Uniprot
K154	Acetylation	Uniprot
K155	Acetylation	Uniprot
Y168	Phosphorylation	Uniprot
Y169	Phosphorylation	Uniprot
S282	Phosphorylation	Uniprot

Research Backgrounds

Q16281_CNGA3_HUMAN

Function:

Visual signal transduction is mediated by a G-protein coupled cascade using cGMP as second messenger. This protein can be activated by cyclic GMP which leads to an opening of the cation channel and thereby causing a depolarization of cone photoreceptors. Induced a flickering channel gating, weakened the outward rectification in the presence of extracellular calcium, increased sensitivity for L-cis diltiazem and enhanced the cAMP efficacy of the channel when coexpressed with CNGB3 (By similarity). Essential for the generation of light-evoked electrical responses in the red-, green- and blue sensitive cones.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Tissue Specificity:

Prominently expressed in retina.

Subunit Structure:

Tetramer formed of three CNGA3 and one CNGB3 modulatory subunits.

Family&Domains:

The C-terminal coiled-coil domain mediates homotrimerization of CNGA subunits.

Belongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family. CNGA3 subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

· Organismal Systems > Sensory system > Olfactory transduction.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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CNGA3 Antibody - #DF9419