F13B Antibody | Affinity Biosciences

WB
IHC
IF/ICC
Cites

1/4

Western blot analysis F13B using COS7 whole cell lysates.

DF9401 at 1/100 staining mouse liver tissue by IHC-P.

DF9401 staining A549 cells by IF/ICC.

FIGURE 5 Factor XII deficiency inhibits thrombus formation in vivo.

Product:	F13B Antibody
Catalog:	DF9401
Description:	Rabbit polyclonal antibody to F13B
Application:	WB IHC IF/ICC
Reactivity:	Human, Monkey
Prediction:	Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog
Mol.Wt.:	76kDa; 76kD(Calculated).
Uniprot:	P05160
RRID:	AB_2842597

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Monkey

Prediction:

Pig(100%), Zebrafish(83%), Bovine(86%), Sheep(100%), Rabbit(86%), Dog(100%)

Clonality:

Polyclonal

Specificity:

F13B Antibody detects endogenous levels of total F13B.

RRID:

AB_2842597
Cite Format: Affinity Biosciences Cat# DF9401, RRID:AB_2842597.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Coagulation factor XIII, A2 polypeptide; Coagulation factor XIII, B polypeptide; F13A2; F13B; FXIIIB;

Immunogens

Immunogen:

Uniprot:

P05160(F13B_HUMAN) >>Visit HPA database.

Gene(ID):

F13B(2165)

Sequence:

P05160 F13B_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRLKNLTFIIILIISGELYAEEKPCGFPHVENGRIAQYYYTFKSFYFPMSIDKKLSFFCLAGYTTESGRQEEQTTCTTEGWSPEPRCFKKCTKPDLSNGYISDVKLLYKIQENMRYGCASGYKTTGGKDEEVVQCLSDGWSSQPTCRKEHETCLAPELYNGNYSTTQKTFKVKDKVQYECATGYYTAGGKKTEEVECLTYGWSLTPKCTKLKCSSLRLIENGYFHPVKQTYEEGDVVQFFCHENYYLSGSDLIQCYNFGWYPESPVCEGRRNRCPPPPLPINSKIQTHSTTYRHGEIVHIECELNFEIHGSAEIRCEDGKWTEPPKCIEGQEKVACEEPPFIENGAANLHSKIYYNGDKVTYACKSGYLLHGSNEITCNRGKWTLPPECVENNENCKHPPVVMNGAVADGILASYATGSSVEYRCNEYYLLRGSKISRCEQGKWSSPPVCLEPCTVNVDYMNRNNIEMKWKYEGKVLHGDLIDFVCKQGYDLSPLTPLSELSVQCNRGEVKYPLCTRKESKGMCTSPPLIKHGVIISSTVDTYENGSSVEYRCFDHHFLEGSREAYCLDGMWTTPPLCLEPCTLSFTEMEKNNLLLKWDFDNRPHILHGEYIEFICRGDTYPAELYITGSILRMQCDRGQLKYPRCIPRQSTLSYQEPLRT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Sheep

100

Dog

100

Bovine

Rabbit

Zebrafish

Horse

Chicken

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05160 As Substrate

P05160

Site	PTM Type	Source
N162	N-Glycosylation	Uniprot
T182	Phosphorylation	Uniprot
S373	Phosphorylation	Uniprot
Y512	Phosphorylation	Uniprot
N545	N-Glycosylation	Uniprot
Y643	Phosphorylation	Uniprot
T661	Phosphorylation	Uniprot

Research Backgrounds

P05160_F13B_HUMAN

Function:

The B chain of factor XIII is not catalytically active, but is thought to stabilize the A subunits and regulate the rate of transglutaminase formation by thrombin.

Subcellular Location:

Secreted.

Subunit Structure:

Tetramer of two A chains (F13A1) and two B (F13B) chains.

Research Fields

· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)

References

1). Cationic Zinc is Required for Factor XII Recruitment and Activation by Stimulated Platelets and for Thrombus Formation in vivo. JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2020 (PubMed: 32542960) [IF=10.4]

Application: IF/ICC Species: Mouse Sample:

FIGURE 5 Factor XII deficiency inhibits thrombus formation in vivo. A, Wild-type (WT) or FXII knockout mice (FXII KO) mice underwent quantitative assessment of thrombus formation in the cremaster arteriole laser injury model (platelet accumulation in red, fibrin formation in green). Representative intravital images are shown, together with median curves and area under the curve (AUC) distributions for platelet accumulation (B-C) and fibrin formation (D-E).

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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F13B Antibody - #DF9401