Product: F13B Antibody
Catalog: DF9401
Description: Rabbit polyclonal antibody to F13B
Application: WB IHC IF/ICC
Reactivity: Human, Monkey
Prediction: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 76kDa; 76kD(Calculated).
Uniprot: P05160
RRID: AB_2842597

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Monkey
Prediction:
Pig(100%), Zebrafish(83%), Bovine(86%), Sheep(100%), Rabbit(86%), Dog(100%)
Clonality:
Polyclonal
Specificity:
F13B Antibody detects endogenous levels of total F13B.
RRID:
AB_2842597
Cite Format: Affinity Biosciences Cat# DF9401, RRID:AB_2842597.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Coagulation factor XIII, A2 polypeptide; Coagulation factor XIII, B polypeptide; F13A2; F13B; FXIIIB;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MRLKNLTFIIILIISGELYAEEKPCGFPHVENGRIAQYYYTFKSFYFPMSIDKKLSFFCLAGYTTESGRQEEQTTCTTEGWSPEPRCFKKCTKPDLSNGYISDVKLLYKIQENMRYGCASGYKTTGGKDEEVVQCLSDGWSSQPTCRKEHETCLAPELYNGNYSTTQKTFKVKDKVQYECATGYYTAGGKKTEEVECLTYGWSLTPKCTKLKCSSLRLIENGYFHPVKQTYEEGDVVQFFCHENYYLSGSDLIQCYNFGWYPESPVCEGRRNRCPPPPLPINSKIQTHSTTYRHGEIVHIECELNFEIHGSAEIRCEDGKWTEPPKCIEGQEKVACEEPPFIENGAANLHSKIYYNGDKVTYACKSGYLLHGSNEITCNRGKWTLPPECVENNENCKHPPVVMNGAVADGILASYATGSSVEYRCNEYYLLRGSKISRCEQGKWSSPPVCLEPCTVNVDYMNRNNIEMKWKYEGKVLHGDLIDFVCKQGYDLSPLTPLSELSVQCNRGEVKYPLCTRKESKGMCTSPPLIKHGVIISSTVDTYENGSSVEYRCFDHHFLEGSREAYCLDGMWTTPPLCLEPCTLSFTEMEKNNLLLKWDFDNRPHILHGEYIEFICRGDTYPAELYITGSILRMQCDRGQLKYPRCIPRQSTLSYQEPLRT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Sheep
100
Dog
100
Bovine
86
Rabbit
86
Zebrafish
83
Horse
71
Chicken
50
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05160 As Substrate

Site PTM Type Enzyme
N162 N-Glycosylation
T182 Phosphorylation
S373 Phosphorylation
Y512 Phosphorylation
N545 N-Glycosylation
Y643 Phosphorylation
T661 Phosphorylation

Research Backgrounds

Function:

The B chain of factor XIII is not catalytically active, but is thought to stabilize the A subunits and regulate the rate of transglutaminase formation by thrombin.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Tetramer of two A chains (F13A1) and two B (F13B) chains.

Research Fields

· Organismal Systems > Immune system > Complement and coagulation cascades.   (View pathway)

References

1). Cationic Zinc is Required for Factor XII Recruitment and Activation by Stimulated Platelets and for Thrombus Formation in vivo. JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2020 (PubMed: 32542960) [IF=10.4]

Application: IF/ICC    Species: Mouse    Sample:

FIGURE 5 Factor XII deficiency inhibits thrombus formation in vivo. A, Wild-type (WT) or FXII knockout mice (FXII KO) mice underwent quantitative assessment of thrombus formation in the cremaster arteriole laser injury model (platelet accumulation in red, fibrin formation in green). Representative intravital images are shown, together with median curves and area under the curve (AUC) distributions for platelet accumulation (B-C) and fibrin formation (D-E).

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