CLDN18 Antibody - #DF9392

(3)  
Product: CLDN18 Antibody
Catalog: DF9392
Description: Rabbit polyclonal antibody to CLDN18
Application: WB IHC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit
Mol.Wt.: 28 kDa; 28kD(Calculated).
Uniprot: P56856
RRID: AB_2842588

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(82%), Bovine(83%), Horse(100%), Sheep(92%), Rabbit(92%)
Clonality:
Polyclonal
Specificity:
CLDN18 Antibody detects endogenous levels of total CLDN18.
RRID:
AB_2842588
Cite Format: Affinity Biosciences Cat# DF9392, RRID:AB_2842588.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Claudin 18; Claudin-18; CLD18_HUMAN; CLDN18; SFTA5; SFTPJ;

Immunogens

Immunogen:
Uniprot:

P56856(CLD18_HUMAN) >>Visit HPA database.

Gene(ID):
CLDN18(51208)
Expression:
P56856 CLD18_HUMAN:

Isoform A1: Expression is restricted to the lung (PubMed:19047087). Isoform A2: Expression is restricted to the stomach mucosa where it is predominantly observed in the epithelial cells of the pit region and the base of the gastric glands including exocrine and endocrine cells (at protein level) (PubMed:19047087).

Sequence:
MSTTTCQVVAFLLSILGLAGCIAATGMDMWSTQDLYDNPVTSVFQYEGLWRSCVRQSSGFTECRPYFTILGLPAMLQAVRALMIVGIVLGAIGLLVSIFALKCIRIGSMEDSAKANMTLTSGIMFIVSGLCAIAGVSVFANMLVTNFWMSTANMYTGMGGMVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLAPEETNYKAVSYHASGHSVAYKPGGFKASTGFGSNTKNKKIYDGGARTEDEVQSYPSKHDYV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Sheep
92
Rabbit
92
Bovine
83
Pig
82
Dog
75
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P56856 As Substrate

Site PTM Type Enzyme
Y206 Phosphorylation
S210 Phosphorylation
Y211 Phosphorylation
S214 Phosphorylation
S217 Phosphorylation
Y220 Phosphorylation
T229 Phosphorylation
Y241 Phosphorylation
T247 Phosphorylation
S253 Phosphorylation
Y254 Phosphorylation
S256 Phosphorylation
Y260 Phosphorylation

Research Backgrounds

Function:

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.

Subcellular Location:

Cell junction>Tight junction. Cell membrane>Multi-pass membrane protein.
Note: Localizes to tight junctions in epithelial cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform A1: Expression is restricted to the lung. Isoform A2: Expression is restricted to the stomach mucosa where it is predominantly observed in the epithelial cells of the pit region and the base of the gastric glands including exocrine and endocrine cells (at protein level).

Family&Domains:

Belongs to the claudin family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

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